Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,892,455	G→A	A281T (GCG→ACG)	mdtL →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,892,455	0	G	A	100.0%	122.1 / NA	38	A281T (GCG→ACG)	mdtL	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/17);  total (21/17)

CGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCG  >  NC_000913/3892323‑3892584                                                                                                                                     |                                                                                                                                  cgcgGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGa                                                                                                                             <  1:851490/139‑1 (MQ=255)                       tGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTc                                                                                                       >  2:784318/1‑139 (MQ=255)                                         tCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTCAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                     <  1:677606/139‑1 (MQ=255)                                         tCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCAcc                                                                                                          >  2:282302/1‑118 (MQ=255)                                         tCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCAcc                                                                                                          <  1:282302/118‑1 (MQ=255)                                                               cACGCCATTTGCGCTGGTAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGcc                                                               <  2:976793/139‑1 (MQ=255)                                                                aCGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCg                                                              >  2:37031/1‑139 (MQ=255)                                                                    cATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTa                                                                                >  1:37171/1‑117 (MQ=255)                                                                    cATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTa                                                                                <  2:37171/117‑1 (MQ=255)                                                                       ttGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                     >  2:153734/1‑109 (MQ=255)                                                                       ttGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                     <  1:153734/109‑1 (MQ=255)                                                                              gggAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGt                                                >  1:671680/1‑139 (MQ=255)                                                                                gAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGttt                                              <  1:1103183/139‑1 (MQ=255)                                                                                           ccACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGa                                   >  1:1121934/1‑139 (MQ=255)                                                                                              cgtacgtTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGa                                >  1:486371/1‑139 (MQ=255)                                                                                                         gatCACCTCGCTGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTc                             <  2:867937/131‑1 (MQ=255)                                                                                                         gatCACCTCGCTGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTc                             >  1:867937/1‑131 (MQ=255)                                                                                                         gatCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTa                     >  2:243513/1‑139 (MQ=255)                                                                                                         gatCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTa                     >  2:216056/1‑139 (MQ=255)                                                                                                            cACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAggg                  >  2:1039494/1‑139 (MQ=255)                                                                                                             aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTgcgc                                                                <  2:853985/92‑1 (MQ=255)                                                                                                             aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTgcgc                                                                >  1:853985/1‑92 (MQ=255)                                                                                                             aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGc                 >  1:944517/1‑139 (MQ=255)                                                                                                             aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGc                 >  2:1108273/1‑139 (MQ=255)                                                                                                             aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGc                 >  1:939474/1‑139 (MQ=255)                                                                                                             aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGc                 <  1:784318/139‑1 (MQ=255)                                                                                                               cTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                          <  1:849202/64‑1 (MQ=255)                                                                                                               cTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                          >  2:849202/1‑64 (MQ=255)                                                                                                               cTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGgt                                          <  2:1035716/112‑1 (MQ=255)                                                                                                               cTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGgt                                          >  1:1035716/1‑112 (MQ=255)                                                                                                               cTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCg               >  2:372297/1‑139 (MQ=255)                                                                                                                 cGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGtt             >  1:857438/1‑139 (MQ=255)                                                                                                                 cGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGtt             >  2:246464/1‑139 (MQ=255)                                                                                                                            ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg  <  1:1108273/139‑1 (MQ=255)                                                                                                                            ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg  <  1:37031/139‑1 (MQ=255)                                                                                                                            ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg  <  1:216056/139‑1 (MQ=255)                                                                                                                            ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg  <  1:243513/139‑1 (MQ=255)                                                                                                                            ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg  <  2:857438/139‑1 (MQ=255)                                                                                                                                 ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                          <  2:988942/46‑1 (MQ=255)                                                                                                                                 ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                          >  1:988942/1‑46 (MQ=255)                                                                                                                                 ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtc                                                                                           >  2:848399/1‑45 (MQ=255)                                                                                                                                 ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtc                                                                                           <  1:848399/45‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  CGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCG  >  NC_000913/3892323‑3892584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑