Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,956,875	G→A	*515* (TAG→TAA)	ilvA →	l‑threonine dehydratase, biosynthetic, also known as threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,956,875	0	G	A	100.0%	47.0 / NA	16	*515* (TAG→TAA)	ilvA	l‑threonine dehydratase, biosynthetic, also known as threonine deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/10);  total (6/10)

CGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCG  >  NC_000913/3956763‑3956994                                                                                                                 |                                                                                                                        cgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGcgcttcgct                                                                                               >  2:794249/1‑139 (MQ=255)                 ccATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGaa                                                                                                                     >  1:278250/1‑101 (MQ=255)                 ccATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGaa                                                                                                                     <  2:278250/101‑1 (MQ=255)                  cATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGcgcttcg                                                                                                 >  1:221751/1‑120 (MQ=255)                  cATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGcgcttcg                                                                                                 <  2:221751/120‑1 (MQ=255)                      aaCCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGaa                                                                                                                     >  1:422301/1‑96 (MQ=255)                      aaCCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGaa                                                                                                                     <  2:422301/96‑1 (MQ=255)                      aaCCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                          <  1:152295/139‑1 (MQ=255)                                         gCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCgg                                                       <  1:794249/139‑1 (MQ=255)                                           tGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCa                                                     <  2:185622/139‑1 (MQ=255)                                                       gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTa                                                                                             >  1:891498/1‑87 (MQ=255)                                                       gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTa                                                                                             <  2:891498/87‑1 (MQ=255)                                                                   acgacgAAACCAATACCCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACACCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTa                             <  2:815693/139‑1 (MQ=255)                                                                                              ggTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCTATTCGCATATCAGGCTTACCCGCGCGACAACGGTATTTGTGGTTCGGCAAAATCTTCCAGAATGTCTCGATTAGCGGTTCATGTAGCCGCTTTTTCTacg  <  2:281521/139‑1 (MQ=255)                                                                                                         gCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATg                                      <  1:965357/92‑1 (MQ=255)                                                                                                         gCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATg                                      >  2:965357/1‑92 (MQ=255)                                                                                                                 |                                                                                                                        CGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCG  >  NC_000913/3956763‑3956994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑