Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,976,605 T→C F110L (TTT→CTT)  wzxE → O‑antigen translocase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,976,6050TC94.1% 111.3 / ‑1.9 34F110L (TTT→CTT) wzxEO‑antigen translocase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (16/16);  minor base G (1/1);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTAT  >  NC_000913/3976470‑3976728
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tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTg                                                                                                                          >  2:474250/1‑139 (MQ=255)
    ttGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTaa                                                                                                                      >  2:971992/1‑139 (MQ=255)
     tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGGTTGGTAAt                                                                                                                     >  2:797038/1‑139 (MQ=255)
                      aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAgggg                                                                                                      <  2:881619/137‑1 (MQ=255)
                      aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAgggg                                                                                                      >  1:881619/1‑137 (MQ=255)
                      aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCt                                                                                                    >  1:18956/1‑139 (MQ=255)
                      aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCT‑GTTTTTGTGCTGGCAGCTGCGCCAATCAACCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTg                                                                                                   >  1:542587/1‑139 (MQ=255)
                             aCAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATAc                                                                                                                   >  2:907077/1‑117 (MQ=255)
                             aCAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATAc                                                                                                                   <  1:907077/117‑1 (MQ=255)
                             aCAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGt                                                                                             >  2:179621/1‑139 (MQ=255)
                                   gcgcCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTg                                                                                       >  2:334966/1‑139 (MQ=255)
                                      ccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATAcc                                                                                                                  <  1:397773/109‑1 (MQ=255)
                                      ccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATAcc                                                                                                                  >  2:397773/1‑109 (MQ=255)
                                            ggTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGGTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                              <  1:797038/139‑1 (MQ=255)
                                            ggTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTa                                                                                                                       >  1:629312/1‑98 (MQ=255)
                                            ggTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTa                                                                                                                       <  2:629312/98‑1 (MQ=255)
                                                     ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                     <  2:18956/139‑1 (MQ=255)
                                                      tcatcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGTGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                    <  1:797469/139‑1 (MQ=255)
                                                        atcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTa                                                                                                                       <  2:32495/86‑1 (MQ=255)
                                                        atcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTa                                                                                                                       >  1:32495/1‑86 (MQ=255)
                                                                tGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                          <  1:179621/139‑1 (MQ=255)
                                                                 ggTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGc                                                         >  1:451134/1‑139 (MQ=255)
                                                                                tACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCaaa                                                                          <  2:222569/107‑1 (MQ=255)
                                                                                tACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCaaa                                                                          >  1:222569/1‑107 (MQ=255)
                                                                                       aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                     <  2:424599/105‑1 (MQ=255)
                                                                                       aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                     >  1:424599/1‑105 (MQ=255)
                                                                                        tGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                  <  1:11687/139‑1 (MQ=255)
                                                                                                   tttGTGCTGGCAGCTGCGCCAATCAACCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                       <  2:542587/139‑1 (MQ=255)
                                                                                                          tGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGc                >  1:612713/1‑139 (MQ=255)
                                                                                                              aGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGt            <  2:804137/139‑1 (MQ=255)
                                                                                                                  gcgcCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATg        <  2:347707/139‑1 (MQ=255)
                                                                                                                    gcCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCg      <  1:971992/139‑1 (MQ=255)
                                                                                                                        aTCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTAt  <  2:612713/139‑1 (MQ=255)
                                                                                                                        aTCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTAt  >  1:157269/1‑139 (MQ=255)
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TACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTAT  >  NC_000913/3976470‑3976728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑