Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,967	C→T	H264H (CAC→CAT)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,967	0	C	T	100.0%	187.7 / NA	57	H264H (CAC→CAT)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/27);  total (30/27)

ATCACTGGTGATGTTATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAC  >  NC_000913/344841‑345082                                                                                                                               |                                                                                                                    aTCACTGGTGATGTTATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGc                                                                                                         >  2:730330/1‑139 (MQ=255)                       tctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAgg                                                                                   >  2:249428/1‑139 (MQ=255)                         tGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGt                                                                                 >  1:1121062/1‑139 (MQ=255)                         tGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGt                                                                                 >  1:868276/1‑139 (MQ=255)                                gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                      >  2:350939/1‑127 (MQ=255)                                gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                      <  1:350939/127‑1 (MQ=255)                                gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCAcc                                                                           >  2:855841/1‑138 (MQ=255)                                gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCAcc                                                                           <  1:855841/138‑1 (MQ=255)                                     aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAg                                                                     >  1:634066/1‑139 (MQ=255)                                     aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAg                                                                     >  1:73175/1‑139 (MQ=255)                                             aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                             <  2:73175/139‑1 (MQ=255)                                                 agaTGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                         >  2:399610/1‑139 (MQ=255)                                                 agaTGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                         >  1:252108/1‑139 (MQ=255)                                                     gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                       <  2:1027199/121‑1 (MQ=255)                                                     gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                       >  1:1027199/1‑121 (MQ=255)                                                         cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                       <  1:1049338/101‑1 (MQ=255)                                                         cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                       >  2:1049338/1‑101 (MQ=255)                                                                      atgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTaca                                                                                                     >  1:731418/1‑74 (MQ=255)                                                                      atgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTaca                                                                                                     <  2:731418/74‑1 (MQ=255)                                                                          atatCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                <  1:730330/139‑1 (MQ=255)                                                                               tAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                           >  1:149306/1‑139 (MQ=255)                                                                                  tAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                        <  2:634066/139‑1 (MQ=255)                                                                                  tAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                        <  2:1121062/139‑1 (MQ=255)                                                                                   agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                        >  2:937589/1‑138 (MQ=255)                                                                                   agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                        <  1:937589/138‑1 (MQ=255)                                                                                    gagTGCATGAATTAGCTGACAGATATTATCATGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                      <  2:868276/139‑1 (MQ=255)                                                                                    gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                      <  1:320402/139‑1 (MQ=255)                                                                                     agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatc                     <  1:399610/139‑1 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATTCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                            <  2:650665/98‑1 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTaca                                                                                                     >  1:366295/1‑57 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTaca                                                                                                     <  2:366295/57‑1 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                            >  1:650665/1‑98 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                        >  2:690448/1‑134 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                        <  1:690448/134‑1 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                      <  1:338492/136‑1 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                      >  2:338492/1‑136 (MQ=255)                                                                                       tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAAGGCTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                   >  1:695539/1‑139 (MQ=255)                                                                                        gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                     <  2:1076464/104‑1 (MQ=255)                                                                                        gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                     >  1:1076464/1‑104 (MQ=255)                                                                                         cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                   <  1:371645/89‑1 (MQ=255)                                                                                         cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                   >  2:371645/1‑89 (MQ=255)                                                                                               ttAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                       <  2:982333/79‑1 (MQ=255)                                                                                               ttAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                       >  1:982333/1‑79 (MQ=255)                                                                                                      aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTaatt                                                                                           >  1:982025/1‑49 (MQ=255)                                                                                                      aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTaatt                                                                                           <  2:982025/52‑4 (MQ=255)                                                                                                      aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                   >  2:1134184/1‑76 (MQ=255)                                                                                                      aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                   <  1:1134184/76‑1 (MQ=255)                                                                                                      aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa    >  2:373724/1‑139 (MQ=255)                                                                                                        aGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAc  <  1:373724/139‑1 (MQ=255)                                                                                                                    gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                      <  1:955738/43‑1 (MQ=255)                                                                                                                    gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                      >  2:955738/1‑43 (MQ=255)                                                                                                                    gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCa                       >  2:11486/1‑106 (MQ=255)                                                                                                                    gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCa                       <  1:11486/106‑1 (MQ=255)                                                                                                                     tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                              >  1:960689/1‑98 (MQ=255)                                                                                                                     tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                              >  1:918973/1‑98 (MQ=255)                                                                                                                     tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                              <  2:918973/98‑1 (MQ=255)                                                                                                                     tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                              <  2:960689/98‑1 (MQ=255)                                                                                                                               |                                                                                                                    ATCACTGGTGATGTTATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAC  >  NC_000913/344841‑345082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑