Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,054,439 A→G T280T (ACT→ACC glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,054,4390AG100.0% 97.6 / NA 28T280T (ACT→ACCglnGfused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/14);  total (14/14)

CGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAAT  >  NC_000913/4054311‑4054574
                                                                                                                                |                                                                                                                                       
cgcgcCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGa                                                                                                                               >  1:279177/1‑139 (MQ=255)
cgcgcCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGa                                                                                                                               >  2:490376/1‑139 (MQ=255)
   gcCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATaa                                                                                                                            <  2:939372/139‑1 (MQ=255)
                               cgcgCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCg                                                                                                                                >  1:483110/1‑107 (MQ=255)
                               cgcgCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCg                                                                                                                                <  2:483110/107‑1 (MQ=255)
                                       ggcggcAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCaa                                                                                        <  2:663366/139‑1 (MQ=255)
                                                                        tGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCa                                                                                                  <  1:52330/96‑1 (MQ=255)
                                                                        tGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCa                                                                                                  >  2:52330/1‑96 (MQ=255)
                                                                                  ccTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAGTCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGt                                             >  1:436281/1‑139 (MQ=255)
                                                                                     cACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTg                                          >  2:860091/1‑139 (MQ=255)
                                                                                      aCGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGc                                         <  2:279177/139‑1 (MQ=255)
                                                                                         gAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGcaca                                      <  1:291596/139‑1 (MQ=255)
                                                                                          aaCTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGa                                                                          <  2:965403/102‑1 (MQ=255)
                                                                                          aaCTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGa                                                                          >  1:965403/1‑102 (MQ=255)
                                                                                            cTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAAcgcg                                                                                    <  2:983940/90‑1 (MQ=255)
                                                                                            cTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAAcgcg                                                                                    >  1:983940/1‑90 (MQ=255)
                                                                                                    ccTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATcc                                                                                                                 <  2:1072061/53‑1 (MQ=255)
                                                                                                    ccTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATcc                                                                                                                 >  1:1072061/1‑53 (MQ=255)
                                                                                                    ccTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCat                           >  1:224609/1‑139 (MQ=255)
                                                                                                             gCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTaa                                                                                >  2:291579/1‑77 (MQ=255)
                                                                                                             gCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTaa                                                                                <  1:291579/77‑1 (MQ=255)
                                                                                                               tGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAAttt                >  2:60260/1‑139 (MQ=255)
                                                                                                                         tCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAAcgcg                                              <  2:486774/99‑1 (MQ=255)
                                                                                                                         tCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAAcgcg                                              >  1:486774/1‑99 (MQ=255)
                                                                                                                         tCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGc                                <  1:963081/113‑1 (MQ=255)
                                                                                                                         tCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGc                                >  2:963081/1‑113 (MQ=255)
                                                                                                                         tCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaa      <  1:860091/139‑1 (MQ=255)
                                                                                                                         tCTGGTGGGTGGCAGCGATAACCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTAACCGTCTGCCAGCACGCGCGGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATCtcgtcgggcaa      <  2:659833/139‑6 (MQ=255)
                                                                                                                             gtgggtggCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACTCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaataat  >  2:670354/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                       
CGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAAT  >  NC_000913/4054311‑4054574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: