Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,075,454 G→A *262* (TAG→TAA yihW → putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,075,4540GA100.0% 74.6 / NA 24*262* (TAG→TAAyihWputative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/13);  total (11/13)

ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGG  >  NC_000913/4075318‑4075575
                                                                                                                                        |                                                                                                                         
aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                         >  2:504462/1‑139 (MQ=255)
aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                         >  2:589848/1‑139 (MQ=255)
                tGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAc                                                                                                         <  2:1084796/139‑1 (MQ=255)
                 gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                        <  1:504462/139‑1 (MQ=255)
                                    aGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcac                                                                                     <  2:218166/139‑1 (MQ=255)
                                                 ctTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCa                                                                        <  2:816618/139‑1 (MQ=255)
                                                                                        cTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAagg                                 >  2:16257/1‑139 (MQ=255)
                                                                                                   gCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                      >  2:702774/1‑139 (MQ=255)
                                                                                                     cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATa                                                                                              >  2:917005/1‑65 (MQ=255)
                                                                                                     cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATa                                                                                              <  1:917005/65‑1 (MQ=255)
                                                                                                       aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctct                  <  1:589848/139‑1 (MQ=255)
                                                                                                             aaTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt            <  2:931930/139‑1 (MQ=255)
                                                                                                             aaTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCACGAAAGGAGGCAAAAATGCTCTATATCt            <  1:702774/139‑1 (MQ=255)
                                                                                                                       ccccAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAgg  >  2:107737/1‑139 (MQ=255)
                                                                                                                                 aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                           >  1:563106/1‑72 (MQ=255)
                                                                                                                                 aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                           <  2:563106/72‑1 (MQ=255)
                                                                                                                                 aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaa                         >  1:424217/1‑106 (MQ=255)
                                                                                                                                 aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaa                         <  2:424217/106‑1 (MQ=255)
                                                                                                                                 aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                      <  1:981652/109‑1 (MQ=255)
                                                                                                                                 aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                      >  2:981652/1‑109 (MQ=255)
                                                                                                                                  cgcgTAAATTTTTGTGTCCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCa                                                                        <  1:1018485/58‑1 (MQ=255)
                                                                                                                                  cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCa                                                                        >  2:1018485/1‑58 (MQ=255)
                                                                                                                                  cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGaaa                                   >  2:846950/1‑95 (MQ=255)
                                                                                                                                  cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGaaa                                   <  1:846950/95‑1 (MQ=255)
                                                                                                                                        |                                                                                                                         
ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGG  >  NC_000913/4075318‑4075575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: