Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,991	G→A	*272* (TAG→TAA)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,991	0	G	A	98.7%	249.6 / ‑6.6	77	*272* (TAG→TAA)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (39/37);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344863‑345125                                                                                                                                 |                                                                                                                                       tctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAgg                                                                                                                              >  2:249428/1‑139 (MQ=255)   tGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGt                                                                                                                            >  1:868276/1‑139 (MQ=255)   tGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGt                                                                                                                            >  1:1121062/1‑139 (MQ=255)          gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                                 >  2:350939/1‑127 (MQ=255)          gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                                 <  1:350939/127‑1 (MQ=255)          gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCAcc                                                                                                                      <  1:855841/138‑1 (MQ=255)          gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCAcc                                                                                                                      >  2:855841/1‑138 (MQ=255)               aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAg                                                                                                                >  1:634066/1‑139 (MQ=255)               aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAg                                                                                                                >  1:73175/1‑139 (MQ=255)                       aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        <  2:73175/139‑1 (MQ=255)                           agaTGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                                                                    >  1:252108/1‑139 (MQ=255)                           agaTGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                                                                    >  2:399610/1‑139 (MQ=255)                               gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  1:1027199/1‑121 (MQ=255)                               gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  2:1027199/121‑1 (MQ=255)                                   cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                                  <  1:1049338/101‑1 (MQ=255)                                   cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                                  >  2:1049338/1‑101 (MQ=255)                                                    atatCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                           <  1:730330/139‑1 (MQ=255)                                                         tAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                      >  1:149306/1‑139 (MQ=255)                                                            tAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  2:634066/139‑1 (MQ=255)                                                            tAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  2:1121062/139‑1 (MQ=255)                                                             agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  1:937589/138‑1 (MQ=255)                                                             agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  2:937589/1‑138 (MQ=255)                                                              gagTGCATGAATTAGCTGACAGATATTATCATGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  2:868276/139‑1 (MQ=255)                                                              gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  1:320402/139‑1 (MQ=255)                                                               agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatc                                                                <  1:399610/139‑1 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATTCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                       <  2:650665/98‑1 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                       >  1:650665/1‑98 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  2:690448/1‑134 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  1:690448/134‑1 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  2:338492/1‑136 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  1:338492/136‑1 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAAGGCTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                              >  1:695539/1‑139 (MQ=255)                                                                  gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                                >  1:1076464/1‑104 (MQ=255)                                                                  gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                                <  2:1076464/104‑1 (MQ=255)                                                                   cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                              >  2:371645/1‑89 (MQ=255)                                                                   cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                              <  1:371645/89‑1 (MQ=255)                                                                         ttAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  2:982333/79‑1 (MQ=255)                                                                         ttAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  1:982333/1‑79 (MQ=255)                                                                                aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                              <  1:1134184/76‑1 (MQ=255)                                                                                aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                              >  2:1134184/1‑76 (MQ=255)                                                                                aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa                                               >  2:373724/1‑139 (MQ=255)                                                                                  aGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAc                                             <  1:373724/139‑1 (MQ=255)                                                                                              gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                                 <  1:955738/43‑1 (MQ=255)                                                                                              gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                                 >  2:955738/1‑43 (MQ=255)                                                                                              gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCa                                                                  <  1:11486/106‑1 (MQ=255)                                                                                              gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCa                                                                  >  2:11486/1‑106 (MQ=255)                                                                                               tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                                                                         >  1:960689/1‑98 (MQ=255)                                                                                               tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                                                                         <  2:960689/98‑1 (MQ=255)                                                                                               tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                                                                         <  2:918973/98‑1 (MQ=255)                                                                                               tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                                                                         >  1:918973/1‑98 (MQ=255)                                                                                                      gcatgTTAAAATCCGCTTCACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttttt                         >  1:709272/5‑139 (MQ=255)                                                                                                        atgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTca                       <  2:709272/137‑2 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAATGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCGTTTTTCGc                      <  2:529590/138‑1 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                                <  2:875756/64‑1 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                                >  1:875756/2‑65 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                      >  1:1012792/2‑75 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                      <  2:1012792/74‑1 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   >  2:164916/2‑78 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   <  1:164916/77‑1 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGc                      >  1:529590/2‑139 (MQ=255)                                                                                                         tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGc                      >  2:124730/2‑139 (MQ=255)                                                                                                                aTCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATAtt               <  1:124730/139‑1 (MQ=255)                                                                                                                   cGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        >  1:950850/1‑63 (MQ=255)                                                                                                                   cGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        <  2:950850/63‑1 (MQ=255)                                                                                                                    gCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgt           >  2:161619/1‑139 (MQ=255)                                                                                                                     cTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            <  2:470925/89‑1 (MQ=255)                                                                                                                     cTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            >  1:470925/1‑89 (MQ=255)                                                                                                                     cTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgtt          <  1:40329/139‑1 (MQ=255)                                                                                                                           tctTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGagag                                                                                             <  2:881814/50‑1 (MQ=255)                                                                                                                           tctTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGagag                                                                                             >  1:881814/1‑50 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                           <  1:149272/66‑1 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                           >  2:149272/1‑66 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  2:596427/76‑1 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  1:596427/1‑76 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAc                                             >  1:252753/1‑96 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAc                                             <  2:252753/96‑1 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCgg  >  1:769873/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       TCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344863‑345125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑