Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,229	A→G	S143S (AGT→AGC)	yijF ←	DUF1287 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,229	0	A	G	100.0%	155.0 / NA	43	S143S (AGT→AGC)	yijF	DUF1287 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/22);  total (21/22)

CTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATTC  >  NC_000913/4137093‑4137361                                                                                                                                         |                                                                                                                                     cttcttCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCt                                                                                                                                    <  1:242204/139‑1 (MQ=255)       ccTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGtt                                                                                                                              <  2:605168/139‑1 (MQ=255)             cgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCt                                                                                                                        >  1:903860/1‑139 (MQ=255)                     ccAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                <  2:626655/139‑1 (MQ=255)                     ccAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                <  2:903860/139‑1 (MQ=255)                                    aCCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                >  1:742916/1‑124 (MQ=255)                                    aCCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTCATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTTGTGGGGc                                                                                                                <  2:742916/124‑1 (MQ=255)                                     ccAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcg                                                                                                >  1:623871/1‑139 (MQ=255)                                            cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTggg                                                                                                                  >  1:571598/1‑114 (MQ=255)                                            cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTggg                                                                                                                  <  2:571598/114‑1 (MQ=255)                                             gTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCt                                                                                                                            <  1:1120312/103‑1 (MQ=255)                                             gTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCt                                                                                                                            >  2:1120312/1‑103 (MQ=255)                                              tACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCa                                                                                       >  2:1049340/1‑139 (MQ=255)                                                   tCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGttt                                                                                  >  1:689083/1‑139 (MQ=255)                                                      cgGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCa                                                                               >  1:160023/1‑139 (MQ=255)                                                        ggCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGa                                                                             <  2:542996/139‑1 (MQ=255)                                                                 aTCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCAcc                                                                    <  2:623871/139‑1 (MQ=255)                                                                   cTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCg                                                                  >  1:1099785/1‑139 (MQ=255)                                                                    tGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGa                                                                 <  2:160023/139‑1 (MQ=255)                                                                         ccaccCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTg                                                            <  2:178615/139‑1 (MQ=255)                                                                            ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCg                                                                                                               <  1:798213/85‑1 (MQ=255)                                                                            ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCg                                                                                                               >  2:798213/1‑85 (MQ=255)                                                                            ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATc                                                         >  2:288333/1‑139 (MQ=255)                                                                                      tgCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGCTTAGGCACCCGACGGTg                                                            <  1:710663/126‑1 (MQ=255)                                                                                      tgCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTg                                                            >  2:710663/1‑126 (MQ=255)                                                                                           gCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACg                                                               <  1:488642/118‑1 (MQ=255)                                                                                           gCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACg                                                               >  2:488642/1‑118 (MQ=255)                                                                                           gCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCgg                                          >  2:172907/1‑139 (MQ=255)                                                                                            cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCggg                                         >  2:581167/1‑139 (MQ=255)                                                                                               gTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGgcgc                                      <  1:288333/139‑1 (MQ=255)                                                                                                      aGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACt                               >  2:370684/1‑139 (MQ=255)                                                                                                          gCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                 <  2:37914/53‑1 (MQ=255)                                                                                                          gCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                 >  1:37914/1‑53 (MQ=255)                                                                                                                      tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTa               >  1:751024/1‑139 (MQ=255)                                                                                                                      tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTa               <  1:1056111/139‑1 (MQ=255)                                                                                                                        tCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCATCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACt             <  2:751024/139‑1 (MQ=255)                                                                                                                           cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                 <  1:814654/36‑1 (MQ=255)                                                                                                                           cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                 >  2:814654/1‑36 (MQ=38)                                                                                                                           cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAg          >  2:618601/1‑139 (MQ=255)                                                                                                                              gCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCaa       <  2:689083/139‑1 (MQ=255)                                                                                                                               cTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACt             >  2:184908/1‑132 (MQ=255)                                                                                                                               cTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACt             <  1:184908/132‑1 (MQ=255)                                                                                                                                   aTAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAAttc  <  1:1049340/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATTC  >  NC_000913/4137093‑4137361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑