Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,148,681 C→T G529S (GGC→AGC)  eptC ← LPS heptose I phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,148,6810CT100.0% 188.7 / NA 57G529S (GGC→AGC) eptCLPS heptose I phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/30);  total (27/30)

TAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATG  >  NC_000913/4148557‑4148813
                                                                                                                            |                                                                                                                                    
tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                        <  1:785695/139‑1 (MQ=255)
  gggCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAg                                                                                                                      <  2:653678/139‑1 (MQ=255)
   ggCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAgg                                                                                                                     >  2:774114/1‑139 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                        <  1:488814/131‑1 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                        >  2:488814/1‑131 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                        >  1:58221/1‑131 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                        <  2:58221/131‑1 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                         <  1:344460/130‑1 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                         >  2:344460/1‑130 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                >  1:281703/1‑139 (MQ=255)
        tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                >  1:953656/1‑139 (MQ=255)
        tgtgtCGTAATCGACCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                >  2:556599/1‑139 (MQ=255)
                  tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCg                                                                                                      >  2:424512/1‑139 (MQ=255)
                  tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCg                                                                                                      <  2:953656/139‑1 (MQ=255)
                     aTCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCa                                                                                                   <  2:281703/139‑1 (MQ=255)
                       cAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                                 <  2:81538/139‑1 (MQ=255)
                            cGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                         <  1:366427/110‑1 (MQ=255)
                            cGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                         >  2:366427/1‑110 (MQ=255)
                            cGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTa                                                                                            >  1:514978/1‑139 (MQ=255)
                             gTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTAt                                                                                           <  1:343368/139‑1 (MQ=255)
                               ttttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTAttt                                                                                         <  1:774114/139‑1 (MQ=255)
                                tttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTa                                                                                        <  1:189344/139‑1 (MQ=255)
                                    ttATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                >  2:236879/1‑127 (MQ=255)
                                    ttATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                <  1:236879/127‑1 (MQ=255)
                                              tACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                           >  2:569143/1‑106 (MQ=255)
                                              tACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                           <  1:569143/106‑1 (MQ=255)
                                              tACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTg                                                                          >  2:576319/1‑139 (MQ=255)
                                               aCCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGgtg                                                                                                                   >  2:157604/1‑97 (MQ=255)
                                               aCCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGgtg                                                                                                                   <  1:157604/97‑1 (MQ=255)
                                                        gCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                         >  1:673718/1‑82 (MQ=255)
                                                        gCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                         <  2:673718/82‑1 (MQ=255)
                                                         cGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                               >  2:620144/1‑91 (MQ=255)
                                                         cGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                               <  1:620144/91‑1 (MQ=255)
                                                              aGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGa                                                          >  1:544068/1‑139 (MQ=255)
                                                                 ttCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGa                                                       <  2:514978/139‑1 (MQ=255)
                                                                      tGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                                    <  1:534559/89‑1 (MQ=255)
                                                                      tGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                                    >  2:534559/1‑89 (MQ=255)
                                                                           tGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGcc                                             >  2:615130/1‑139 (MQ=255)
                                                                             cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCAt                                           >  2:797817/1‑139 (MQ=255)
                                                                                  tCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                <  2:784598/81‑1 (MQ=255)
                                                                                  tCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                >  1:784598/1‑81 (MQ=255)
                                                                                      cacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGAc                                  <  1:904935/139‑1 (MQ=255)
                                                                                           aaCGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCca                             <  2:544068/139‑1 (MQ=255)
                                                                                           aaCGGGTTGGATCGTAACCGTCGCAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                                       >  1:19239/1‑81 (MQ=255)
                                                                                           aaCGGGTTGGATCGTAACCGTCGCAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                                       <  2:19239/81‑1 (MQ=255)
                                                                                                   ggATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGAc                                  >  1:1064519/1‑126 (MQ=255)
                                                                                                   ggATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGAc                                  <  2:1064519/126‑1 (MQ=255)
                                                                                                        gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                <  1:797817/139‑1 (MQ=255)
                                                                                                         tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa               <  1:424512/139‑1 (MQ=255)
                                                                                                              gtcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa               >  1:830135/1‑134 (MQ=255)
                                                                                                              gtcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa               <  2:830135/134‑1 (MQ=255)
                                                                                                               tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCa                                            >  1:675038/1‑104 (MQ=255)
                                                                                                               tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCa                                            <  2:675038/104‑1 (MQ=255)
                                                                                                                    aGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                <  2:348705/127‑1 (MQ=255)
                                                                                                                    aGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                >  1:348705/1‑127 (MQ=255)
                                                                                                                      aTAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATg  >  2:343464/1‑139 (MQ=255)
                                                                                                                         aGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa               <  2:260630/123‑1 (MQ=255)
                                                                                                                            |                                                                                                                                    
TAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATG  >  NC_000913/4148557‑4148813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: