Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,752	G→A	A161V (GCG→GTG)	lysC ←	lysine‑sensitive aspartokinase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,752	0	G	A	100.0%	156.4 / NA	48	A161V (GCG→GTG)	lysC	lysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/22);  total (26/22)

GGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATC  >  NC_000913/4232616‑4232874                                                                                                                                         |                                                                                                                           ggCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGcacg                                                                                                                          <  1:996132/139‑1 (MQ=255)                    ccTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCAc                                                                                                       <  1:455490/139‑1 (MQ=255)                     cTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACg                                                                                                      >  1:217519/1‑139 (MQ=255)                                       ttGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGt                                                                                    >  2:742311/1‑139 (MQ=255)                                           aCGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGc                                                                                <  2:604517/139‑1 (MQ=255)                                            cGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               <  1:119082/139‑1 (MQ=255)                                                  ttATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACtt                                                                         >  1:1133134/1‑139 (MQ=255)                                                    aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg                                                                                             <  1:227713/117‑1 (MQ=255)                                                    aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg                                                                                             >  2:227713/1‑117 (MQ=255)                                                                       cccTGGGTGATCACTAAGCCTACATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACtgtg                                                    <  1:668101/139‑1 (MQ=255)                                                                        ccTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               <  1:208830/111‑1 (MQ=255)                                                                        ccTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               >  2:208830/1‑111 (MQ=255)                                                                           gggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGc                                                                                <  2:414603/107‑1 (MQ=255)                                                                           gggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGc                                                                                >  1:414603/1‑107 (MQ=255)                                                                                aTCACTAAGCCTTCATTGAGACGTGGGTGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCgg                                                                                            <  1:675928/90‑1 (MQ=255)                                                                                aTCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTg                                                                                      >  2:989227/1‑96 (MQ=255)                                                                                aTCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTg                                                                                      <  1:989227/96‑1 (MQ=255)                                                                                aTCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               <  1:879440/103‑1 (MQ=255)                                                                                aTCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               >  2:879440/1‑103 (MQ=255)                                                                                aTCACTAAGCCTTCATTGAGACGTGGGAGCAGATGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCgg                                                                                            >  2:675928/1‑90 (MQ=255)                                                                                 tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATc                                          >  2:191593/1‑139 (MQ=255)                                                                                 tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATc                                          >  1:158471/1‑139 (MQ=255)                                                                                       aGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                        >  2:908151/1‑119 (MQ=255)                                                                                       aGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                        <  1:908151/119‑1 (MQ=255)                                                                                       aGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTc                                    >  1:317494/1‑139 (MQ=255)                                                                                          cTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               <  2:415271/93‑1 (MQ=255)                                                                                          cTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               >  1:415271/1‑93 (MQ=255)                                                                                           ttCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCgcgc                                <  2:317494/139‑1 (MQ=255)                                                                                            tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               <  1:202003/91‑1 (MQ=255)                                                                                            tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                               >  2:202003/1‑91 (MQ=255)                                                                                             cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAAt                                                                                               >  1:294889/1‑74 (MQ=255)                                                                                             cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAAt                                                                                               <  2:294889/74‑1 (MQ=255)                                                                                             cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGtt                                     <  2:19316/132‑1 (MQ=255)                                                                                             cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGtt                                     >  1:19316/1‑132 (MQ=255)                                                                                             cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAg                              >  2:614846/1‑139 (MQ=255)                                                                                                      gTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTcaac                     >  1:518684/1‑139 (MQ=255)                                                                                                       tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTcaaca                    >  2:52624/1‑139 (MQ=255)                                                                                                        gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt                                                                                           >  2:324787/1‑67 (MQ=255)                                                                                                        gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt                                                                                           <  1:324787/67‑1 (MQ=255)                                                                                                              agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAAcagca             <  2:1133134/139‑1 (MQ=255)                                                                                                                cTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGcc                                                  <  1:912158/100‑1 (MQ=255)                                                                                                                cTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGcc                                                  >  2:912158/1‑100 (MQ=255)                                                                                                                cTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAgg           <  1:742311/139‑1 (MQ=255)                                                                                                                   cAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACTTCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTc        >  2:600204/1‑139 (MQ=255)                                                                                                                      cgcgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                          <  2:410836/70‑1 (MQ=255)                                                                                                                      cgcgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                          >  1:410836/1‑70 (MQ=255)                                                                                                                         ggCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATc  >  2:839761/1‑139 (MQ=255)                                                                                                                          gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGt         >  2:805795/1‑131 (MQ=255)                                                                                                                          gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGt         <  1:805795/131‑1 (MQ=255)                                                                                                                                         |                                                                                                                           GGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATC  >  NC_000913/4232616‑4232874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑