Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	37,030	A→G	V265V (GTT→GTC)	caiC ←	putative crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	37,030	0	A	G	100.0%	156.4 / NA	44	V265V (GTT→GTC)	caiC	putative crotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

TCACAAAACGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACG  >  NC_000913/36899‑37157                                                                                                                                    |                                                                                                                                tCACAAAACGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACAGCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTCCCAGCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTgg                                                                                                                          <  2:864805/139‑1 (MQ=255)      aaaCGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCgg                                                                                                                     <  2:587784/139‑1 (MQ=255)         cGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTAc                                                                                                                  <  2:976872/139‑1 (MQ=255)                 tttCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTAc                                                                                                          <  2:407733/139‑1 (MQ=255)                        cTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGTGGTACTTCTGTAc                                                                                                          >  1:292934/1‑132 (MQ=255)                        cTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGTGGTACTTCTGTAc                                                                                                          <  2:292934/132‑1 (MQ=255)                                               tCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTAc                                                                                                          <  2:610454/109‑1 (MQ=255)                                               tCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATAATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTAc                                                                                                          >  1:610454/1‑109 (MQ=255)                                                      ccGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                      <  1:254495/138‑1 (MQ=255)                                                      ccGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                      >  2:254495/1‑138 (MQ=255)                                                       cGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACg                                                                                                                              >  1:134791/1‑81 (MQ=255)                                                       cGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACg                                                                                                                              <  2:134791/81‑1 (MQ=255)                                                       cGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                      >  1:118330/1‑121 (MQ=255)                                                       cGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                      <  2:118330/121‑1 (MQ=255)                                                           ggCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTa                                                                                                                   >  1:206278/1‑88 (MQ=255)                                                           ggCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTa                                                                                                                   <  2:206278/88‑1 (MQ=255)                                                                tgtTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAgg                                                           >  1:361053/1‑139 (MQ=255)                                                                      tGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAg                                                                   >  2:697030/1‑125 (MQ=255)                                                                      tGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGAACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCac                                                                   <  1:697030/125‑2 (MQ=255)                                                                         tCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCgg                                                  <  2:361053/139‑1 (MQ=255)                                                                          cGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCt                                                                                                        <  1:882421/84‑1 (MQ=255)                                                                          cGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCt                                                                                                        >  2:882421/1‑84 (MQ=255)                                                                          cGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGc                                                 >  2:69530/1‑139 (MQ=255)                                                                          cGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGc                                                 >  2:602050/1‑139 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCgg                                                                                                                     >  2:988531/1‑69 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCgg                                                                                                                     <  1:988531/69‑1 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAg                                               >  1:888909/1‑139 (MQ=255)                                                                               gCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGaaa                                            >  2:200781/1‑139 (MQ=255)                                                                                                  tCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATcg                                >  1:835192/1‑132 (MQ=255)                                                                                                  tCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATcg                                <  2:835192/132‑1 (MQ=255)                                                                                                   cAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTa                        <  1:751908/139‑1 (MQ=255)                                                                                                   cAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTa                        <  2:888909/139‑1 (MQ=255)                                                                                                     aCGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCgg                                                                                                                     >  2:130938/1‑44 (MQ=255)                                                                                                     aCGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCgg                                                                                                                     <  1:130938/44‑1 (MQ=255)                                                                                                     aCGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTaca                      <  1:69530/139‑1 (MQ=255)                                                                                                             atcatcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAAt              >  1:926585/1‑139 (MQ=255)                                                                                                                    tcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATg       <  2:926585/139‑1 (MQ=255)                                                                                                                         aTACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACg  >  2:716093/1‑139 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                      >  2:1073418/1‑65 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                      <  1:1073418/65‑1 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGc                                                       <  2:415493/80‑1 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGc                                                       >  1:415493/1‑80 (MQ=255)                                                                                                                               tCGGTGACGGTG‑‑GCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCaa               <  1:966236/118‑1 (MQ=255)                                                                                                                               tCGGTGACGGTG‑‑GCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCaa               >  2:966236/1‑118 (MQ=255)                                                                                                                                    |                                                                                                                                TCACAAAACGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACG  >  NC_000913/36899‑37157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑