Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,377,189 C→T H105H (CAC→CAT
*178* (TAG→TAA
sugE →
blc ←
multidrug efflux system protein
outer membrane lipoprotein cell division and growth lipocalin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,377,1890CT100.0% 113.5 / NA 36H105H (CAC→CAT
*178* (TAG→TAA
sugE
blc
multidrug efflux system protein
outer membrane lipoprotein cell division and growth lipocalin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

TGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGG  >  NC_000913/4377056‑4377321
                                                                                                                                     |                                                                                                                                    
tgtgGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCattaact                                                                                                                                 >  2:80982/1‑139 (MQ=255)
         gTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctg                                                                                                                        >  1:769445/1‑139 (MQ=255)
                   aGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCaa                                                                                                              >  2:45411/1‑139 (MQ=255)
                                       gCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCaaa                                                                                          <  1:80982/139‑1 (MQ=255)
                                            gtgCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCtt                                                                                     >  2:721827/1‑139 (MQ=255)
                                            gtgCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCtt                                                                                     >  2:707702/1‑139 (MQ=255)
                                              gctgctCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTcc                                                                                   <  1:254236/139‑1 (MQ=255)
                                                     ggTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTcg                                                                            <  1:285192/139‑1 (MQ=255)
                                                       tGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAgg                                                                                                                           <  2:986318/90‑1 (MQ=255)
                                                       tGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAgg                                                                                                                           >  1:986318/1‑90 (MQ=255)
                                                       tGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTcgcg                                                                          <  1:707702/139‑1 (MQ=255)
                                                           tCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTg                                                                      <  1:182709/139‑1 (MQ=255)
                                                                  aCCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAt                                                               <  1:1076739/139‑1 (MQ=255)
                                                                   cccGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtc                                                              <  2:769445/139‑1 (MQ=255)
                                                                        tGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTg                                                         >  1:152837/1‑139 (MQ=255)
                                                                            ccTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTa                                                                                                                   >  1:775192/1‑77 (MQ=255)
                                                                            ccTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTa                                                                                                                   <  2:775192/77‑1 (MQ=255)
                                                                            ccTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                                   <  2:863735/93‑1 (MQ=255)
                                                                            ccTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                                   >  1:863735/1‑93 (MQ=255)
                                                                                               aTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgct                                                                                                                      >  1:1037481/1‑55 (MQ=255)
                                                                                               aTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgct                                                                                                                      <  2:756178/55‑1 (MQ=255)
                                                                                               aTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgct                                                                                                                      <  2:1037481/55‑1 (MQ=255)
                                                                                               aTCGTATTGGGGATTATTGCTCTGAAACTCAGCACTCATTAACTACCAGgctgct                                                                                                                      >  1:756178/1‑55 (MQ=255)
                                                                                                 cGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGc                                >  1:1108405/1‑139 (MQ=255)
                                                                                                       ggggATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCgg                          <  1:45411/139‑1 (MQ=255)
                                                                                                         ggATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGga                        <  1:721827/139‑1 (MQ=255)
                                                                                                             tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGagag                     <  2:11414/138‑1 (MQ=255)
                                                                                                             tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGagag                     >  1:11414/1‑138 (MQ=255)
                                                                                                                  gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCca               >  1:962376/1‑139 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGa                                                                         <  2:831114/70‑1 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGa                                                                         >  1:831114/1‑70 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtctc                                                            >  1:83170/1‑83 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtctc                                                            <  2:83170/83‑1 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGt                                              >  2:1079798/1‑97 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGt                                              <  1:1079798/97‑1 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCggg                         <  2:314965/118‑1 (MQ=255)
                                                                                                                             aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCggg                         >  1:314965/1‑118 (MQ=255)
                                                                                                                               cactcattAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCgg  >  1:779784/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGG  >  NC_000913/4377056‑4377321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: