Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,389,934	A→G	Y143H (TAC→CAC)	psd ←	phosphatidylserine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,389,934	0	A	G	100.0%	161.6 / NA	45	Y143H (TAC→CAC)	psd	phosphatidylserine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/22);  total (23/22)

AAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATC  >  NC_000913/4389802‑4390057                                                                                                                                     |                                                                                                                            aaaTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                       >  2:518727/1‑139 (MQ=255)       cgcgTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGga                                                                                                                 <  1:518727/139‑1 (MQ=255)                              ttCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                          >  1:877869/1‑139 (MQ=255)                                   cACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                     >  2:571083/1‑139 (MQ=255)                                                gTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCg                                                                        <  2:207928/139‑1 (MQ=255)                                                 tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                       >  1:712648/1‑139 (MQ=255)                                                     aTGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATc                                                                   <  2:579347/139‑1 (MQ=255)                                                             cGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGtt                                                           >  1:1054790/1‑139 (MQ=255)                                                                 gaagaGATCGCCCGGCACGTAGATAATCTCACGCAGAATACCGTTGCAAGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgcct                                                       >  2:114854/1‑139 (MQ=255)                                                                   agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGGGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                          >  1:804768/1‑118 (MQ=255)                                                                   agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGGGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                          <  2:804768/118‑1 (MQ=255)                                                                       aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACg                                                                                             <  1:127654/95‑1 (MQ=255)                                                                       aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACg                                                                                             >  2:127654/1‑95 (MQ=255)                                                                       aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                     <  2:61344/103‑1 (MQ=255)                                                                       aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                     >  1:61344/1‑103 (MQ=255)                                                                           cccGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                          >  2:365489/1‑78 (MQ=255)                                                                           cccGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                          <  1:365489/78‑1 (MQ=255)                                                                               gCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTc                                         <  2:1008134/139‑1 (MQ=255)                                                                                      gATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                  <  1:444437/139‑1 (MQ=255)                                                                                            ctcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTca                                                                                                   <  2:1102757/68‑1 (MQ=255)                                                                                            ctcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTca                                                                                                   >  1:1102757/1‑68 (MQ=255)                                                                                             tcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTtgt                           <  2:877869/139‑1 (MQ=255)                                                                                             tcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTtgt                           >  1:57381/1‑139 (MQ=255)                                                                                                     aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCtt                                          >  1:855219/1‑116 (MQ=255)                                                                                                     aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCtt                                          <  2:855219/116‑1 (MQ=255)                                                                                                     aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTg                   <  1:114854/139‑1 (MQ=255)                                                                                                            ttGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCa            <  2:712648/139‑1 (MQ=255)                                                                                                             tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTg                   >  1:465078/1‑131 (MQ=255)                                                                                                             tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTg                   <  2:465078/131‑1 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgcct                                                       <  1:38045/93‑1 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgcct                                                       >  2:38045/1‑93 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagcag                                               >  2:737951/1‑101 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagcag                                               <  1:737951/101‑1 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAgg                                     >  1:90038/1‑111 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAgg                                     <  2:90038/111‑1 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                  >  2:639559/1‑114 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                  <  1:639559/114‑1 (MQ=255)                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa         >  1:342869/1‑139 (MQ=255)                                                                                                                      tgtgTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGc                 <  2:795745/124‑1 (MQ=255)                                                                                                                      tgtgTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGc                 >  1:795745/1‑124 (MQ=255)                                                                                                                      tgtgTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTAtc  >  2:710954/1‑139 (MQ=255)                                                                                                                              cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                     >  2:842353/1‑48 (MQ=255)                                                                                                                              cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                     <  1:842353/48‑1 (MQ=255)                                                                                                                              cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGtt                             <  1:57597/104‑1 (MQ=255)                                                                                                                              cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGtt                             >  2:57597/1‑104 (MQ=255)                                                                                                                                     |                                                                                                                            AAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATC  >  NC_000913/4389802‑4390057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑