Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,834	G→A	*105* (TAG→TAA)	priB →	primosomal protein N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,834	0	G	A	100.0%	109.1 / NA	34	*105* (TAG→TAA)	priB	primosomal protein N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

ACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTAT  >  NC_000913/4425710‑4425969                                                                                                                             |                                                                                                                                        acaGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGtt                                                                                                                           >  2:695142/1‑139 (MQ=255)        aaCGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                                    >  1:574371/1‑139 (MQ=255)             tCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAAcc                                                                                                                                       <  2:164220/115‑1 (MQ=255)             tCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAAcc                                                                                                                                       >  1:164220/1‑115 (MQ=255)               ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                                 <  2:532325/119‑1 (MQ=255)               ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                                 >  1:532325/1‑119 (MQ=255)               ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGc                                                                                                             >  2:795788/1‑139 (MQ=255)               ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGc                                                                                                             >  2:602997/1‑139 (MQ=255)                               ttCAGGGGTTCATTTCATGGCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCa                                                                                             >  1:755320/1‑139 (MQ=255)                               ttCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAGTTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGttcc                                                                                              >  2:754262/1‑136 (MQ=255)                               ttCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTc                                                                                              <  1:754262/138‑1 (MQ=255)                               ttCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCa                                                                                             <  1:602997/139‑1 (MQ=255)                                        tcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                    <  1:695142/139‑1 (MQ=255)                                        tcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                    <  1:795788/139‑1 (MQ=255)                                        tcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                    <  2:755320/139‑1 (MQ=255)                                                      aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                      <  1:466355/139‑1 (MQ=255)                                                      aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                      <  2:574371/139‑1 (MQ=255)                                                                  ggACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGat                                                          >  2:1072326/1‑139 (MQ=255)                                                                    aCTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGatat                                                        >  1:1120714/1‑139 (MQ=255)                                                                         gCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTa                                                   <  1:1072326/139‑1 (MQ=255)                                                                              aTGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTg                                              <  1:858187/139‑1 (MQ=255)                                                                                       cATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAc                                     >  2:990240/1‑139 (MQ=255)                                                                                           ccGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCa                                 >  1:761318/1‑139 (MQ=255)                                                                                              aGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                    <  2:594866/85‑1 (MQ=255)                                                                                              aGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                    >  1:594866/1‑85 (MQ=255)                                                                                                         ttgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                      <  2:232611/88‑1 (MQ=255)                                                                                                         ttgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                      >  1:232611/1‑88 (MQ=255)                                                                                                             tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                    >  2:297043/1‑70 (MQ=255)                                                                                                             tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                    <  1:297043/70‑1 (MQ=255)                                                                                                                 ttCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTccc           >  2:992772/1‑139 (MQ=255)                                                                                                                      gagaCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAgtc      >  1:23089/1‑137 (MQ=255)                                                                                                                      gagaCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGcc      >  2:816955/1‑139 (MQ=255)                                                                                                                         aCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTa   <  1:816955/139‑1 (MQ=255)                                                                                                                          cTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTAt  <  1:990240/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                        ACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTAT  >  NC_000913/4425710‑4425969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑