Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,549,061	A→G	Y85C (TAT→TGT)	fimH →	minor component of type 1 fimbriae

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,549,061	0	A	G	100.0%	178.0 / NA	49	Y85C (TAT→TGT)	fimH	minor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/21);  total (28/21)

TTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCG  >  NC_000913/4548929‑4549188                                                                                                                                     |                                                                                                                                tttATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTggcgg                                                                                                                           <  2:806551/139‑1 (MQ=255)          aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATc                                                                                                                  >  2:195231/1‑139 (MQ=255)              tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAAt                                                                                                              >  1:730425/1‑139 (MQ=255)               gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAAtt                                                                                                             >  1:594111/1‑139 (MQ=255)                     gtcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCg                                                                                                       >  2:1131818/1‑139 (MQ=255)                                             gTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTa                                                                               >  1:133280/1‑139 (MQ=255)                                                  ggATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTAt                                                                          <  1:480519/139‑1 (MQ=255)                                                   gATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTggc                                                                                                                             >  1:816240/1‑87 (MQ=255)                                                   gATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTggc                                                                                                                             <  2:816240/87‑1 (MQ=255)                                                     tCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCa                                                                       <  1:1131818/139‑1 (MQ=255)                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAAcgccgc                                              >  1:554780/1‑139 (MQ=255)                                                                                 gATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgcg                                           <  2:133280/139‑1 (MQ=255)                                                                                           aaaCCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATaa                                 >  2:606097/1‑139 (MQ=255)                                                                                                aTTACAGACTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCga                            >  2:720661/1‑139 (MQ=255)                                                                                                        cTATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGAt                    <  2:594111/139‑1 (MQ=255)                                                                                                         tATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAg                                                                                 <  1:1027006/77‑1 (MQ=255)                                                                                                         tATGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAg                                                                                 >  2:1027006/1‑77 (MQ=255)                                                                                                          aTGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                              <  1:490856/79‑1 (MQ=255)                                                                                                          aTGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                              >  2:490856/1‑79 (MQ=255)                                                                                                           tGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGaaa                                                    <  2:62401/104‑1 (MQ=255)                                                                                                           tGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGaaa                                                    >  1:62401/1‑104 (MQ=255)                                                                                                           tGTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAg                 >  2:879181/1‑139 (MQ=255)                                                                                                            gTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAg                                                                                 <  1:920745/74‑1 (MQ=255)                                                                                                            gTCACACTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAg                                                                                 >  2:920745/1‑74 (MQ=255)                                                                                                                 acTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAAcgcc                                                >  1:1088214/1‑102 (MQ=255)                                                                                                                 acTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAAcgcc                                                <  2:1088214/102‑1 (MQ=255)                                                                                                                 acTGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTgg           >  1:824963/1‑139 (MQ=255)                                                                                                                   tGCAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGcc         >  1:484855/1‑139 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat                                                                                           <  2:615547/55‑1 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat                                                                                           >  1:615547/1‑55 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt                                                                 <  1:381981/81‑1 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt                                                                 >  1:323996/1‑81 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt                                                                 >  2:381981/1‑81 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt                                                                 <  2:323996/81‑1 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgc                                            <  2:1061995/102‑1 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgc                                            >  1:1061995/1‑102 (MQ=255)                                                                                                                     cAACGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCgg       >  1:619254/1‑139 (MQ=255)                                                                                                                        cGAGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTgg    <  2:484855/139‑1 (MQ=255)                                                                                                                          aGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAAtt                               <  1:743398/110‑1 (MQ=255)                                                                                                                          aGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAAtt                               >  2:743398/1‑110 (MQ=255)                                                                                                                          aGGCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGgcg  >  1:176083/1‑139 (MQ=255)                                                                                                                           ggCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTaa                                                                                              >  2:97872/1‑46 (MQ=255)                                                                                                                           ggCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTaa                                                                                              <  1:97872/46‑1 (MQ=255)                                                                                                                           ggCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGt                                                                                      <  2:133343/54‑1 (MQ=255)                                                                                                                           ggCTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGt                                                                                      >  1:133343/1‑54 (MQ=255)                                                                                                                             cTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                                                                                               >  2:569426/1‑43 (MQ=255)                                                                                                                             cTCGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                                                                                               <  1:569426/43‑1 (MQ=255)                                                                                                                               cGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                              <  2:538802/58‑1 (MQ=255)                                                                                                                               cGGCTTGTGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                              >  1:538802/1‑58 (MQ=255)                                                                                                                                     |                                                                                                                                TTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCG  >  NC_000913/4548929‑4549188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑