Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,464	C→A	L129F (TTG→TTT)	yjiM ←	putative 2‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,464	0	C	A	100.0%	113.5 / NA	37	L129F (TTG→TTT)	yjiM	putative 2‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/22);  total (15/22)

TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAG  >  NC_000913/4565332‑4565593                                                                                                                                     |                                                                                                                                  tttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc                                                                                                                             <  2:559863/139‑1 (MQ=255) tttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc                                                                                                                             <  2:451912/139‑1 (MQ=255)           attgCAGCGCGCAGAGCATCTTCGCTAATCCCGTGCCCAAACCGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTTGGCTTTCCATATCGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATgta                                                                                                                   <  2:680962/136‑3 (MQ=255)           aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                   <  2:459875/139‑1 (MQ=255)           aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                   <  1:33849/139‑1 (MQ=255)           aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                   <  2:851899/139‑1 (MQ=255)                    cgcAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTaa                                                                                                          >  2:868841/1‑139 (MQ=255)                      caaaccATCTTCGCTAATCTCGTGCCGAAATCGTTCTTCTACCGATTTTTGCAAGCGCAGTATCTCGGCTCTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAAc                                                                                                        <  2:933231/134‑1 (MQ=255)                            aTCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGcc                                                                                                  <  1:868841/139‑1 (MQ=255)                                        ctcGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACt                                                                                                       >  1:331613/1‑122 (MQ=255)                                        ctcGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACt                                                                                                       <  2:331613/122‑1 (MQ=255)                                              cccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTc                                                                                >  1:403919/1‑139 (MQ=255)                                                    aCGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTg                                                                          <  2:711826/139‑1 (MQ=255)                                                                cGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAggtg                                                              <  1:651266/139‑1 (MQ=255)                                                                  ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                   <  1:457347/84‑1 (MQ=255)                                                                  ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                   >  2:457347/1‑84 (MQ=255)                                                                    ttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                   >  2:665699/1‑82 (MQ=255)                                                                    ttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAACTGCATCACATGaa                                                                                                                   <  1:665699/82‑1 (MQ=255)                                                                         cAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                     <  1:92033/139‑1 (MQ=255)                                                                                            ttCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGaaa                                                                                                                                 >  1:671640/1‑44 (MQ=255)                                                                                               cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAaagtaga                               >  1:482578/1‑139 (MQ=255)                                                                                                    cgcacgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAaagtagaagtag                          <  2:482578/139‑1 (MQ=255)                                                                                                        cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACa                                                                                                                 >  1:8453/1‑48 (MQ=255)                                                                                                        cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACa                                                                                                                 <  2:8453/48‑1 (MQ=255)                                                                                                        cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGttt                                                          >  2:557150/1‑103 (MQ=255)                                                                                                        cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGttt                                                          <  1:557150/103‑1 (MQ=255)                                                                                                          cgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCAt                    >  2:702766/1‑139 (MQ=255)                                                                                                              gCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAggg                        <  2:439320/131‑1 (MQ=255)                                                                                                              gCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAggg                        >  1:439320/1‑131 (MQ=255)                                                                                                                         aaCGCTGTTGGGAAATTGCATCACATGAACAGGCtt                                                                                                            <  2:175840/36‑1 (MQ=255)                                                                                                                         aaCGCTGTTGGGAAATTGCATCACATGAACAGGCtt                                                                                                            >  1:175840/1‑36 (MQ=255)                                                                                                                           cGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGG‑TTTGCCGAAg  >  1:1054723/1‑139 (MQ=255)                                                                                                                              tGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAg  <  1:1025904/137‑1 (MQ=255)                                                                                                                              tGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAg  >  2:1025904/1‑137 (MQ=255)                                                                                                                               gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTC‑TTTTGCCGTCGCAGGTGGTTTTACCGACCAACAGATCCg                                        >  1:35126/1‑97 (MQ=255)                                                                                                                               gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTC‑TTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCg                                        <  2:35126/97‑1 (MQ=255)                                                                                                                                ttGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa                                                                  >  2:413122/1‑71 (MQ=255)                                                                                                                                ttGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa                                                                  <  1:413122/71‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAG  >  NC_000913/4565332‑4565593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑