Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,569,309	G→A	pseudogene (312/921 nt)	yjiP →	pseudogene, transposase_31 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,569,309	0	G	A	100.0%	142.8 / NA	45	pseudogene (312/921 nt)	yjiP	pseudogene, transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/20);  total (25/20)

ATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGG  >  NC_000913/4569185‑4569441                                                                                                                             |                                                                                                                                     atatTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCa                                                                                                                        <  1:319517/139‑1 (MQ=255)    ttttATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGcc                                                                                                                                  <  1:1102391/126‑1 (MQ=255)    ttttATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGcc                                                                                                                                  >  2:1102391/1‑126 (MQ=255)    ttttATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATagag                                                                                                                          <  2:874150/134‑1 (MQ=255)    ttttATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATagag                                                                                                                          >  1:874150/1‑134 (MQ=255)       tATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATaa                                                                                                                  >  1:243430/1‑139 (MQ=255)         tGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAAc                                                                                                                >  1:628/1‑139 (MQ=255)              ggTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAg                                                                                                           >  1:454652/1‑139 (MQ=255)                  aaGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGc                                                                                                       <  2:243430/139‑1 (MQ=255)                          cgAAGGCGATGGCTAAATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTg                                                                                               >  2:978490/1‑139 (MQ=255)                                      cTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCGATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATg                                                                                   <  1:978490/139‑1 (MQ=255)                                               tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                          >  1:684558/1‑139 (MQ=255)                                                 tggtgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCa                                                                        >  2:170966/1‑139 (MQ=255)                                                    tgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                                     >  2:432592/1‑139 (MQ=255)                                                    tgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                                     >  2:90252/1‑139 (MQ=255)                                                     gATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                                    <  2:628/139‑1 (MQ=255)                                                     gATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                                    >  2:78527/1‑139 (MQ=255)                                                      aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCata                                                                                                                               <  1:8035/79‑1 (MQ=255)                                                      aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCata                                                                                                                               >  2:8035/1‑79 (MQ=255)                                                                 gagCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCt                                                        <  2:454652/139‑1 (MQ=255)                                                                 gagCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCt                                                        <  1:733244/139‑1 (MQ=255)                                                                    ccGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCAtata                                                                                                                             >  2:539218/1‑67 (MQ=255)                                                                    ccGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCAtata                                                                                                                             <  1:539218/67‑1 (MQ=255)                                                                       cgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTAcccc                                                  >  2:209206/1‑139 (MQ=255)                                                                        gAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCt                                                 <  1:432592/139‑1 (MQ=255)                                                                         aGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTg                                                >  1:418916/1‑139 (MQ=255)                                                                                    atGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATg                                                                                   >  2:430428/1‑93 (MQ=255)                                                                                    atGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATg                                                                                   <  1:430428/93‑1 (MQ=255)                                                                                      ggCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                          <  1:342525/100‑1 (MQ=255)                                                                                      ggCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                          >  2:342525/1‑100 (MQ=255)                                                                                             cGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGt                                                                                             <  1:482759/74‑1 (MQ=255)                                                                                             cGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGt                                                                                             >  2:482759/1‑74 (MQ=255)                                                                                                    tGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATCTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGc                     >  2:72287/1‑139 (MQ=255)                                                                                                      cgcTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGccg                   <  1:78527/139‑1 (MQ=255)                                                                                                                   cGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGTTGGCTGGACGAATTTGCCGACCCGACTACCGc      >  2:527872/1‑139 (MQ=255)                                                                                                                   cGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGc      >  1:1077029/1‑139 (MQ=255)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGc                                                                                                       >  2:703421/1‑40 (MQ=255)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGc                                                                                                       <  1:703421/40‑1 (MQ=39)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                                    <  2:39668/59‑1 (MQ=255)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                                    >  1:39668/1‑59 (MQ=255)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTcc                                            >  2:321447/1‑99 (MQ=255)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTcc                                            <  1:321447/99‑1 (MQ=255)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCAc    <  1:170966/139‑1 (MQ=255)                                                                                                                     gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCAc    >  1:420960/1‑139 (MQ=255)                                                                                                                       gATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACgg  <  2:722685/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                     ATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGG  >  NC_000913/4569185‑4569441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑