Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,585,322 C→T T480T (ACG→ACA hsdR ← endonuclease R Type I restriction enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,585,3220CT100.0% 90.0 / NA 29T480T (ACG→ACAhsdRendonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

GTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCA  >  NC_000913/4585202‑4585443
                                                                                                                        |                                                                                                                         
gTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTtaccggta                                                                                                         <  2:297375/139‑1 (MQ=255)
   aaTACGCGTATCTTCAAACGCGCCCAGCGCCTGTTAGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTtaccggtaccg                                                                                                      >  2:986383/1‑139 (MQ=255)
                       cgcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAt                                                                                  >  1:451716/1‑139 (MQ=255)
                        gcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc                                                                                 <  1:806611/139‑1 (MQ=255)
                         cccAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc                                                                                       <  2:887885/132‑1 (MQ=255)
                         cccAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc                                                                                       >  1:887885/1‑132 (MQ=255)
                                           agagAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGa                                                              <  1:986383/139‑1 (MQ=255)
                                                 cggcggTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc                                                                                 <  1:249723/114‑1 (MQ=255)
                                                 cggcggTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc                                                                                 >  2:249723/1‑114 (MQ=255)
                                                                aaGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGa                                         <  1:511757/139‑1 (MQ=255)
                                                                    gaATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGc                                     <  1:881586/139‑1 (MQ=255)
                                                                     aaTGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa                                    >  2:488883/1‑139 (MQ=255)
                                                                       tGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATc                                  <  1:488883/139‑1 (MQ=255)
                                                                        gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa                                    <  1:458070/136‑1 (MQ=255)
                                                                        gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa                                    >  2:458070/1‑136 (MQ=255)
                                                                        gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCt                                 >  2:178829/1‑139 (MQ=255)
                                                                         cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttg                                                                            <  1:914575/95‑1 (MQ=255)
                                                                         cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttg                                                                            >  2:914575/1‑95 (MQ=255)
                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTa                                                                                                                        <  1:905775/50‑1 (MQ=255)
                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTa                                                                                                                        >  2:905775/1‑50 (MQ=255)
                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTc                               >  2:427468/1‑139 (MQ=255)
                                                                                          cTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAgg               <  2:337440/139‑1 (MQ=255)
                                                                                                  ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca                                                                                      <  1:94678/60‑1 (MQ=255)
                                                                                                  ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca                                                                                      >  2:94678/1‑60 (MQ=255)
                                                                                                  ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt                                                                          <  2:669434/72‑1 (MQ=255)
                                                                                                  ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt                                                                          >  1:669434/1‑72 (MQ=255)
                                                                                                  ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctc       >  1:436826/1‑139 (MQ=255)
                                                                                                     ggAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctcgct    <  1:427468/139‑1 (MQ=255)
                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCCCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCa  >  2:494742/1‑139 (MQ=255)
                                                                                                                        |                                                                                                                         
GTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCA  >  NC_000913/4585202‑4585443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: