Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,202	G→A	*242* (TAG→TAA)  E15K (GAA→AAA)	yjjQ → bglJ →	putative transcriptional regulator bgl operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,202	0	G	A	100.0%	148.4 / NA	46	*242* (TAG→TAA)  E15K (GAA→AAA)	yjjQ bglJ	putative transcriptional regulator bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

GCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGA  >  NC_000913/4604075‑4604293                                                                                                                                |                                                                                            gCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  >  2:637436/1‑139 (MQ=255) gCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  <  1:272431/139‑1 (MQ=255)                    aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                        >  2:943292/1‑130 (MQ=255)                    aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                        <  1:943292/130‑1 (MQ=255)                       gATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTgaga                                                            >  2:1058276/1‑139 (MQ=255)                           aaTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGttt                                                        >  2:1014542/1‑139 (MQ=255)                                cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt                                                                            >  2:472367/1‑114 (MQ=255)                                cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt                                                                            <  1:472367/114‑1 (MQ=255)                                cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATc                                                                      <  2:921285/120‑1 (MQ=255)                                cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATc                                                                      >  1:921285/1‑120 (MQ=255)                                cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCa                                                   >  2:965800/1‑139 (MQ=255)                                  cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                             <  2:986344/111‑1 (MQ=255)                                  cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                             >  1:986344/1‑111 (MQ=255)                                  cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa                                                 >  2:278173/1‑139 (MQ=255)                                                gaACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa                                   <  1:637436/139‑1 (MQ=255)                                                 aacttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAc                                  >  1:1104731/1‑139 (MQ=255)                                                 aacttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAc                                  >  2:714052/1‑139 (MQ=255)                                                   cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc                                >  2:1132806/1‑139 (MQ=255)                                                     taacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  >  2:745064/1‑87 (MQ=255)                                                     taacttGCCGTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  <  1:745064/87‑1 (MQ=255)                                                         ttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATaa                          <  1:100397/139‑1 (MQ=255)                                                             cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                      <  2:300189/139‑1 (MQ=255)                                                             cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                      <  2:1104731/139‑1 (MQ=255)                                                             cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                      <  1:965800/139‑1 (MQ=255)                                                             cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                      <  1:1132806/139‑1 (MQ=255)                                                             cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                      <  1:1058276/139‑1 (MQ=255)                                                              ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                      <  1:377900/122‑1 (MQ=255)                                                              ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                      >  2:377900/1‑122 (MQ=255)                                                              ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                     <  1:278173/139‑1 (MQ=255)                                                                cTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  >  2:782539/1‑76 (MQ=255)                                                                cTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  <  1:782539/76‑1 (MQ=255)                                                                            aTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagtag                                                                          <  2:223617/72‑1 (MQ=255)                                                                            aTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagtag                                                                          >  1:223617/1‑72 (MQ=255)                                                                            aTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGt       <  1:714052/139‑1 (MQ=255)                                                                             tGCCGCATAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  >  2:743415/1‑63 (MQ=255)                                                                             tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGc                                                                                     <  2:323375/60‑1 (MQ=255)                                                                             tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGc                                                                                     >  1:323375/1‑60 (MQ=255)                                                                             tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                  <  1:743415/63‑1 (MQ=255)                                                                             tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATg                                                                               >  2:83269/1‑66 (MQ=255)                                                                             tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATg                                                                               <  1:83269/66‑1 (MQ=255)                                                                             tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                             <  2:334691/68‑1 (MQ=255)                                                                             tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                             >  1:334691/1‑68 (MQ=255)                                                                                               aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt                                     <  2:192763/90‑1 (MQ=255)                                                                                               aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt                                     >  1:192763/1‑90 (MQ=255)                                                                                                         agaagaGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGa  <  1:162803/115‑1 (MQ=255)                                                                                                         agaagaGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGa  >  2:162803/1‑115 (MQ=255)                                                                                                                                |                                                                                            GCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGA  >  NC_000913/4604075‑4604293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑