| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 369,076 | A→G | K156E (AAA→GAA) | mhpA → | 3‑(3‑hydroxyphenyl)propionate hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 369,076 | 0 | A | G | 100.0% | 107.1 / NA | 31 | K156E (AAA→GAA) | mhpA | 3‑(3‑hydroxyphenyl)propionate hydroxylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/12); total (19/12) | |||||||||||
AACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGA > NC_000913/368944‑369202 | aaCGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACgg > 1:543520/1‑139 (MQ=255) aTTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGc > 1:507897/1‑139 (MQ=255) ccGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCggg > 1:696107/1‑139 (MQ=255) ggTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATa > 2:866571/1‑139 (MQ=255) gAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTgg < 2:309364/139‑1 (MQ=255) aGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTa > 1:215531/1‑139 (MQ=255) aGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTa > 2:22524/1‑139 (MQ=255) ggTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGcc > 2:177441/1‑139 (MQ=255) gtgtCGCGTTTTACGAATGTGCGCTGATTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGAGCAGCGGGAAATAGACAAAGCCCAGTGGCTGGTAGCCt > 2:479945/1‑139 (MQ=255) gtCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCtgt < 2:543520/139‑1 (MQ=255) tCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCtgtg > 1:177443/1‑139 (MQ=255) ttttCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGt > 1:21410/1‑139 (MQ=255) ccGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAg < 1:866571/139‑1 (MQ=255) cGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGc < 1:177441/139‑1 (MQ=255) cgcTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTg > 1:92203/1‑139 (MQ=255) cgcTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTg > 1:572677/1‑139 (MQ=255) gctTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCg > 1:854167/1‑139 (MQ=255) tttCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCAc < 2:21410/139‑1 (MQ=255) cgcgAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGa < 1:459701/62‑1 (MQ=255) cgcgAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGa > 2:459701/1‑62 (MQ=255) cTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCa < 1:316656/76‑1 (MQ=255) cTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCa > 2:316656/1‑76 (MQ=255) gAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGc > 2:292369/1‑107 (MQ=255) gAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGc < 1:292369/107‑1 (MQ=255) ttCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAg > 1:969853/1‑139 (MQ=255) aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGt < 2:968708/41‑1 (MQ=255) aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGt > 1:968708/1‑41 (MQ=255) aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGa < 2:507897/139‑1 (MQ=255) aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGa < 2:969853/139‑1 (MQ=255) gCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAg > 2:813196/1‑46 (MQ=255) gCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAg < 1:813196/46‑1 (MQ=255) | AACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGA > NC_000913/368944‑369202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |