Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,168,350 Δ1 bp coding (483/633 nt) ycfQ ← repressor for bhsA(ycfR)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,168,3480G.100.0% 228.1 / NA 56coding (485/633 nt)ycfQrepressor for bhsA(ycfR)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/31);  total (25/31)

GGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAA  >  NC_000913/1168216‑1168481
                                                                                                                                    |                                                                                                                                     
ggCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTc                                                                                                                                >  2:1665028/1‑139 (MQ=255)
    aTAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcg                                                                                                                            <  1:1263949/139‑1 (MQ=255)
      aaaCGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcgcg                                                                                                                          >  2:1683841/1‑139 (MQ=255)
      aaaCGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcgcg                                                                                                                          <  1:703435/139‑1 (MQ=255)
      aaaCGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcgcg                                                                                                                          <  2:1478622/139‑1 (MQ=255)
       aaCGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcgcgc                                                                                                                         <  2:1601815/139‑1 (MQ=255)
         cGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCgcgcgc                                                                                                                       <  2:1144249/139‑1 (MQ=255)
                              tGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCgcgcgc                                                                                                                       >  2:345820/1‑118 (MQ=255)
                              tGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCgcgcgc                                                                                                                       <  1:345820/118‑1 (MQ=255)
                              tGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGATATAAAAActg                                                                                                  >  1:211727/1‑139 (MQ=255)
                                        tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                        <  1:1683841/139‑1 (MQ=255)
                                        tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                        <  2:211727/139‑1 (MQ=255)
                                                gATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTg                                                                                >  2:711238/1‑139 (MQ=255)
                                                                 gcTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCg                                                                                                                 >  2:1242170/1‑89 (MQ=255)
                                                                 gcTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCg                                                                                                                 <  1:1242170/89‑1 (MQ=255)
                                                                    gATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCa                                                            <  2:668241/139‑1 (MQ=255)
                                                                    gATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCa                                                            <  2:32009/139‑1 (MQ=255)
                                                                            tacccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTGACAGTGGGGC‑GGATTTCCCCGCGCGTTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTg                                                    <  2:136712/137‑1 (MQ=255)
                                                                               ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCg                                                 >  2:709926/1‑139 (MQ=255)
                                                                                  gAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGata                                              <  1:1665028/139‑1 (MQ=255)
                                                                                       ataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTcc                                         <  1:1565621/139‑1 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCgcgcgc                                                                                                                       <  2:670176/60‑1 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCgcgcgc                                                                                                                       >  1:670176/1‑60 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCt                                                                                                                      <  1:628107/61‑1 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCt                                                                                                                      >  2:628107/1‑61 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGtt                                                                                                               <  2:793230/68‑1 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGtt                                                                                                               >  1:793230/1‑68 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATaaaaa                                                                                                     >  1:1060677/1‑78 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATaaaaa                                                                                                     <  2:1060677/78‑1 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCaaa                                                                                           <  2:1509560/88‑1 (MQ=255)
                                                                                        taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCaaa                                                                                           >  1:1509560/1‑88 (MQ=255)
                                                                                           aaTTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGaa                                     <  1:711238/139‑1 (MQ=255)
                                                                                             ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                        >  2:560337/1‑86 (MQ=255)
                                                                                             ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                        <  1:560337/86‑1 (MQ=255)
                                                                                             ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                   >  1:561327/1‑139 (MQ=255)
                                                                                             ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                   >  1:493125/1‑139 (MQ=255)
                                                                                             ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTACATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                   >  2:1320611/1‑139 (MQ=255)
                                                                                              tAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt                                  <  2:561327/139‑1 (MQ=255)
                                                                                                  gAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcg                                                                                                                            <  1:197626/45‑1 (MQ=255)
                                                                                                  gAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcg                                                                                                                            >  2:197626/1‑45 (MQ=255)
                                                                                                     ttCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGgaga                           >  1:576147/1‑139 (MQ=255)
                                                                                                          ccAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGATATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                               >  1:1144041/1‑114 (MQ=255)
                                                                                                          ccAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                               <  2:1144041/114‑1 (MQ=255)
                                                                                                          ccAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt                                  <  1:675059/127‑1 (MQ=255)
                                                                                                          ccAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt                                  >  2:675059/1‑127 (MQ=255)
                                                                                                            aGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGtt                                                                                                               <  2:1521711/48‑1 (MQ=255)
                                                                                                            aGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGtt                                                                                                               >  1:1521711/1‑48 (MQ=255)
                                                                                                             gATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcgcg                                                                                                                          <  2:190532/36‑1 (MQ=255)
                                                                                                             gATGTGTCACGTCACAGTGGGGC‑GGATTTCCCcgcg                                                                                                                          >  1:190532/1‑36 (MQ=255)
                                                                                                                 tgtCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAAc                                                                                                    <  2:1433767/54‑1 (MQ=255)
                                                                                                                 tgtCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAAc                                                                                                    >  1:1433767/1‑54 (MQ=255)
                                                                                                                   tcacgtcacAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                        >  2:285821/1‑64 (MQ=255)
                                                                                                                   tcacgtcacAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                        <  1:285821/64‑1 (MQ=255)
                                                                                                                    cacgtcacAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgttgt            >  2:1430418/1‑139 (MQ=255)
                                                                                                                     acgtcacAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgttgtt           <  1:709926/139‑1 (MQ=255)
                                                                                                                              tGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTACATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGaa  <  1:1320611/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAA  >  NC_000913/1168216‑1168481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑