Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,696,452 (A)5→4 intergenic (‑381/+10) uidA ← / ← uidR beta‑D‑glucuronidase/transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,696,4480A.98.2% 223.8 / ‑3.2 57intergenic (‑377/+14)uidA/uidRbeta‑D‑glucuronidase/transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (28/28);  minor base G (1/0);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696310‑1696580
                                                                                                                                          |                                                                                                                                    
cGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGa                                                                                                                                      <  2:1357138/139‑1 (MQ=255)
cGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGa                                                                                                                                      >  1:1367560/1‑139 (MQ=255)
       ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:1602388/139‑5 (MQ=255)
       ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  1:1591268/139‑5 (MQ=255)
       ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  1:1679866/139‑5 (MQ=255)
         aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAagagaga                                                                                                                            >  1:1417877/1‑139 (MQ=255)
            gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGaa                                                                                                                         >  1:1675061/1‑139 (MQ=255)
             aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        >  2:1205632/1‑139 (MQ=255)
                 aCTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAgg                                                                                                                    <  2:1367560/139‑1 (MQ=255)
                   tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  2:1417877/139‑1 (MQ=255)
                                      aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCCGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  >  1:1008296/1‑120 (MQ=255)
                                      aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:1641388/108‑5 (MQ=255)
                                      aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               >  1:1641388/1‑104 (MQ=255)
                                      aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                               >  1:263996/1‑139 (MQ=255)
                                      aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCACCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  2:1008296/120‑1 (MQ=255)
                                        tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatc                                                                                             <  1:201467/139‑1 (MQ=255)
                                          aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      <  1:1429473/128‑1 (MQ=255)
                                          aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      >  2:1429473/1‑128 (MQ=255)
                                          aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                           <  2:314553/139‑1 (MQ=255)
                                            tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                         <  2:751522/139‑1 (MQ=255)
                                            tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                         <  1:1205632/139‑1 (MQ=255)
                                            atatttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGGAAAAAGAAATTACGATATGAAACGCGGGCACAGTTTTCAACAACCCGGCGGAAAGGAAGGAGATCAGGGTGGGGGTAAGATACCGCCAAACATcg                                                                                         >  2:1661621/4‑139 (MQ=255)
                                                               cGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAagagaga                                                                                                                            >  2:1156896/1‑85 (MQ=255)
                                                               cGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAagagaga                                                                                                                            <  1:1156896/85‑1 (MQ=255)
                                                                  aGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacga                                                                   >  2:1658218/1‑139 (MQ=255)
                                                                       ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAagagaga                                                                                                                            <  1:334324/77‑1 (MQ=255)
                                                                       ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAagagaga                                                                                                                            >  2:334324/1‑77 (MQ=255)
                                                                       ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGt                                                                                                             >  2:262920/1‑92 (MQ=255)
                                                                       ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGt                                                                                                             <  1:262920/92‑1 (MQ=255)
                                                                       ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCACCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                              >  1:1592754/1‑139 (MQ=255)
                                                                       ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAACAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                              >  2:1150067/1‑139 (MQ=255)
                                                                             aTAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgcg                                                        >  1:393447/1‑139 (MQ=255)
                                                                              tAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaa                                                                                                                                     >  2:1606310/1‑62 (MQ=255)
                                                                              tAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaa                                                                                                                                     <  1:1606310/62‑1 (MQ=255)
                                                                               aaTTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCACCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                      <  2:1592754/139‑1 (MQ=255)
                                                                                  ttttGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAgg                                                   <  2:393447/139‑1 (MQ=255)
                                                                                      gCACAGAGAAATTTCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  2:964892/72‑1 (MQ=255)
                                                                                      gCACAGAGAAATTTCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  >  1:964892/1‑72 (MQ=255)
                                                                                                 ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:1658218/139‑1 (MQ=255)
                                                                                                      atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:943786/44‑5 (MQ=255)
                                                                                                      atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               >  1:943786/1‑40 (MQ=255)
                                                                                                      atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGc                                                                                                                >  2:69718/1‑58 (MQ=255)
                                                                                                      atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGc                                                                                                                <  1:69718/58‑1 (MQ=255)
                                                                                                      atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGtt                                                                                                            <  2:411590/62‑1 (MQ=255)
                                                                                                      atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGtt                                                                                                            >  1:411590/1‑62 (MQ=255)
                                                                                                      atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:263996/139‑1 (MQ=255)
                                                                                                        atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        <  1:215049/48‑1 (MQ=255)
                                                                                                        atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        >  2:215049/1‑48 (MQ=255)
                                                                                                        atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:788014/132‑1 (MQ=255)
                                                                                                        atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    >  2:788014/1‑132 (MQ=255)
                                                                                                                   gCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAg                  >  2:41657/1‑139 (MQ=255)
                                                                                                                    cacaGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAgg                 <  1:41657/139‑1 (MQ=255)
                                                                                                                            tCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               >  1:441432/1‑117 (MQ=255)
                                                                                                                            tCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:441432/117‑1 (MQ=255)
                                                                                                                             cACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  2:1671778/111‑1 (MQ=255)
                                                                                                                             cACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    >  1:1671778/1‑111 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATcc                                                                               <  1:485146/62‑1 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATcc                                                                               >  2:485146/1‑62 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgc                                                         <  2:880120/84‑1 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgc                                                         >  1:880120/1‑84 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCtt                                         <  1:1118012/100‑1 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCtt                                         >  2:1118012/1‑100 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    >  2:1094290/1‑105 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:1094290/105‑1 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:108594/110‑1 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               >  1:108594/1‑110 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:1370590/1‑139 (MQ=255)
                                                                                                                                   cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:499451/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                    
CGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696310‑1696580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: