Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,662 Δ1 bp coding (322/1155 nt) yagA ← CP4‑6 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,6600G.100.0% 123.2 / NA 31coding (324/1155 nt)yagACP4‑6 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (15/16);  total (15/16)

AAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATG  >  NC_000913/281525‑281782
                                                                                                                                       |                                                                                                                          
aaaGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggca                                                                                                                         <  2:966078/139‑3 (MQ=255)
aaaGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggca                                                                                                                         <  2:1368473/139‑3 (MQ=255)
                  cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGgtg                                                                                                                   >  2:888743/1‑126 (MQ=255)
                  cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGgtg                                                                                                                   <  1:888743/126‑1 (MQ=255)
                    tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCg                                                                                                    >  2:1434128/1‑139 (MQ=255)
                       gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGc                                                                                                 <  2:884949/139‑1 (MQ=255)
                       gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGc                                                                                                 <  2:151025/139‑1 (MQ=255)
                         cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCa                                                                                               <  2:1504798/139‑1 (MQ=255)
                         cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCa                                                                                               <  1:636355/139‑1 (MQ=255)
                                       cgcgTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggcatg                                                                                                                       >  1:1024163/1‑98 (MQ=255)
                                       cgcgTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggcatg                                                                                                                       <  2:1024163/102‑5 (MQ=255)
                                                          cccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATg                                                              >  1:278724/1‑139 (MQ=255)
                                                           ccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                             <  1:1434128/139‑1 (MQ=255)
                                                                         cccGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCCTCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGGGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCAt                                               >  2:227476/1‑139 (MQ=255)
                                                                              tGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCa                                                                                               >  1:1515912/1‑86 (MQ=255)
                                                                              tGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCa                                                                                               <  2:1515912/86‑1 (MQ=255)
                                                                               gAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCggg                                                                              >  1:145546/1‑102 (MQ=255)
                                                                               gAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCggg                                                                              <  2:145546/102‑1 (MQ=255)
                                                                                        ggCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGgtgt                                                                                                                  <  1:1141622/57‑1 (MQ=255)
                                                                                        ggCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGgtgt                                                                                                                  >  2:1141622/1‑57 (MQ=255)
                                                                                          cagcagGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATggg                                                   >  1:1023010/1‑118 (MQ=255)
                                                                                          cagcagGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATggg                                                   <  2:1023010/118‑1 (MQ=255)
                                                                                                       cGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGc                                                                                                 >  1:228044/1‑59 (MQ=255)
                                                                                                       cGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGc                                                                                                 <  2:228044/59‑1 (MQ=255)
                                                                                                         ggCCATCAGGTTATGGATGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                             >  2:1444097/1‑93 (MQ=255)
                                                                                                         ggCCATCAGGTTATGGATGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                             <  1:1444097/93‑1 (MQ=255)
                                                                                                          gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATggg                                                   >  2:1630779/1‑102 (MQ=255)
                                                                                                          gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATggg                                                   <  1:1630779/102‑1 (MQ=255)
                                                                                                               cAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAgc                                                                                             <  2:504921/55‑1 (MQ=255)
                                                                                                               cAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAgc                                                                                             >  1:504921/1‑55 (MQ=255)
                                                                                                                      tGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATg  >  2:1317385/1‑139 (MQ=255)
                                                                                                                           ggTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATa                                              >  2:1042387/1‑90 (MQ=255)
                                                                                                                           ggTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATa                                              <  1:1042387/90‑1 (MQ=255)
                                                                                                                             tGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTg                               >  1:1192402/1‑103 (MQ=255)
                                                                                                                             tGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTg                               <  2:1192402/103‑1 (MQ=255)
                                                                                                                                       |                                                                                                                          
AAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATG  >  NC_000913/281525‑281782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: