Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,238,562 (T)7→6 intergenic (+265/‑18) ygjR → / → alx putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,238,5560T.98.3% 235.5 / ‑2.4 60intergenic (+259/‑24)ygjR/alxputative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (30/29);  minor base A (1/0);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NC_000913/3238418‑3238690
                                                                                                                                          |                                                                                                                                      
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                        >  2:596188/1‑139 (MQ=255)
 gtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtt                                                                                                                                       >  2:111639/1‑139 (MQ=255)
    aaaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttt                                                                                                                                    <  2:825131/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   <  1:399532/139‑1 (MQ=255)
            caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                           >  2:70658/1‑139 (MQ=255)
               ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAgg                                                                                                                        <  1:70658/139‑1 (MQ=255)
                                        aTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  1:1278145/116‑1 (MQ=255)
                                        aTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      >  2:1278145/1‑116 (MQ=255)
                                         tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      >  1:712524/1‑115 (MQ=255)
                                         tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  2:712524/115‑1 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                                       >  2:139286/1‑139 (MQ=255)
                                                 cGTATTCCTTTTTTGTTGTAAGTGAGCCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  2:1532511/107‑1 (MQ=255)
                                                 cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa                                                                                      >  2:1019680/1‑139 (MQ=255)
                                                 cGTATTCCTTTTTTGTGGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      >  1:1532511/1‑107 (MQ=255)
                                                             ttgttgTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTc                                                                          >  1:1323656/1‑139 (MQ=255)
                                                                ttgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 >  2:321231/1‑113 (MQ=255)
                                                                ttgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  1:321231/113‑1 (MQ=255)
                                                                          gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCg                                                             >  1:1388825/1‑139 (MQ=255)
                                                                          gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCg                                                             <  1:1019680/139‑1 (MQ=255)
                                                                             cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttt                                                                                                                                    <  1:1359907/66‑1 (MQ=255)
                                                                             cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttt                                                                                                                                    >  2:1359907/1‑66 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                                 >  2:909613/1‑65 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                                 <  1:909613/67‑3 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                           <  1:518931/72‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                           >  2:518931/1‑72 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      >  2:917604/1‑77 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  1:917604/77‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                    <  1:691146/79‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                    >  2:691146/1‑79 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCt                                                                                                                 <  2:1208804/82‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCt                                                                                                                 >  1:1208804/1‑82 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACATTGGCCGTTTTTTATGTGTAAGGATCTTCTATGAATACTATCGGCACGCCGTTGCTATGGGGCGGATTCGCTATTGTTGTCGCCAtt                                                        >  1:1035442/1‑139 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttta                                                                                                                                  <  1:1112152/65‑2 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttta                                                                                                                                  >  2:1112152/1‑64 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGaa                                                                                                            >  1:121212/1‑86 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGaa                                                                                                            <  2:121212/86‑1 (MQ=255)
                                                                                 ccGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  2:1517112/96‑1 (MQ=255)
                                                                                 ccGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 >  1:1517112/1‑96 (MQ=255)
                                                                                    gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   <  2:457913/60‑1 (MQ=255)
                                                                                    gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   >  1:457913/1‑60 (MQ=255)
                                                                                     aaGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGa                                                                                                                       >  1:259134/1‑70 (MQ=255)
                                                                                     aaGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGa                                                                                                                       <  2:259134/70‑1 (MQ=255)
                                                                                     aaGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            >  2:38153/1‑97 (MQ=255)
                                                                                     aaGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  1:38153/97‑1 (MQ=255)
                                                                                         cGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTa                                              <  2:1323656/139‑1 (MQ=255)
                                                                                          gAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTAt                                             <  1:596188/139‑1 (MQ=255)
                                                                                          gAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTAt                                             <  1:111639/139‑1 (MQ=255)
                                                                                           aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      >  1:1544457/1‑65 (MQ=255)
                                                                                           aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  2:1544457/65‑1 (MQ=255)
                                                                                            ggTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCg                                           <  1:14215/139‑1 (MQ=255)
                                                                                              tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  2:1388825/139‑1 (MQ=255)
                                                                                                 aTGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 >  2:1577989/1‑80 (MQ=255)
                                                                                                 aTGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  1:1577989/80‑1 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  1:533234/74‑1 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 >  2:533234/1‑74 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            >  2:1325287/1‑79 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  1:1325287/79‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                    <  2:78232/54‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                    >  1:78232/1‑54 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 >  2:1174962/1‑73 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  1:1174962/73‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                     <  1:56204/133‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                     >  2:56204/1‑133 (MQ=255)
                                                                                                            gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                           >  1:926248/1‑139 (MQ=255)
                                                                                                                           ttCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  2:1586461/59‑1 (MQ=255)
                                                                                                                           ttCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            >  1:1586461/1‑59 (MQ=255)
                                                                                                                                   ttGGCCGATTTTTATGTGTAAGGAACTTCTATGAATACTGTCgg                                                                                                   >  2:503105/1‑44 (MQ=37)
                                                                                                                                   ttGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCgg                                                                                                   <  1:503105/44‑1 (MQ=38)
                                                                                                                                    tGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgac   >  2:752553/1‑139 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGc                                                    <  1:925515/89‑1 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGc                                                    >  2:925515/1‑89 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  >  1:564068/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                      
TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NC_000913/3238418‑3238690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: