Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,925 +A coding (35/1431 nt) yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,9221.A98.2% 177.3 / ‑4.0 57coding (38/1431 nt)yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (28/28);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTC  >  NC_000913/7791‑8054
                                                                                                                                     |                                                                                                                                    
cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagta                                                                                                                               <  1:1436791/139‑5 (MQ=255)
cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                               <  1:160163/139‑5 (MQ=255)
cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                               <  2:549591/139‑5 (MQ=255)
  ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  1:1634453/139‑1 (MQ=255)
  ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:1025395/139‑1 (MQ=255)
   cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                             >  1:1065348/1‑139 (MQ=255)
   cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                             >  1:833628/1‑139 (MQ=255)
        tgtgGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                               >  1:720911/1‑127 (MQ=255)
        tgtgGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                               <  2:720911/131‑5 (MQ=255)
             aTGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGaa                                                                                                                   >  1:797239/1‑139 (MQ=255)
                aaTGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGga                                                                                                                >  1:1267613/1‑139 (MQ=255)
                aaTGCTATTTTTAAGACTTTTGCCAAACCGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaa                                                                                                                                    >  1:426221/1‑118 (MQ=255)
                aaTGCTATTTTTAAGACTTTTGCCAAACCGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaa                                                                                                                                    <  2:426221/118‑1 (MQ=255)
                 aTGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGgag                                                                                                               >  1:864923/1‑139 (MQ=255)
                    cTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              >  1:1204280/1‑121 (MQ=255)
                    cTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:1204280/121‑1 (MQ=255)
                       tttttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGaaaaaa                                                                                                         >  1:1455101/1‑139 (MQ=255)
                            aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                    >  1:1628047/1‑139 (MQ=255)
                            aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                    <  2:1455101/139‑1 (MQ=255)
                             aGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              <  1:1674968/111‑6 (MQ=255)
                             aGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              >  2:1674968/1‑106 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat                                                                                             <  2:833628/139‑1 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat                                                                                             >  2:309107/1‑139 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              <  2:372289/105‑6 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              >  1:1513282/1‑100 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              >  1:372289/1‑100 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              <  2:1513282/105‑6 (MQ=255)
                                       cAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                                                         <  2:864923/139‑1 (MQ=255)
                                           cTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCctct                                                                                     >  1:728755/1‑139 (MQ=255)
                                              gCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATAGCCCTCTTAt                                                                                  >  2:1161900/1‑139 (MQ=255)
                                                     tAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGt                                                                           <  2:24101/139‑1 (MQ=255)
                                                      aGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                              <  2:1001920/119‑1 (MQ=255)
                                                      aGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                              >  1:1001920/1‑119 (MQ=255)
                                                        cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgc                                                                        <  1:309107/139‑1 (MQ=255)
                                                          aaaCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                      <  2:270795/139‑1 (MQ=255)
                                                            aCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat                                                                                             >  1:230218/1‑114 (MQ=255)
                                                            aCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat                                                                                             <  2:230218/114‑1 (MQ=255)
                                                            aCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATg                                                                    <  2:1267613/139‑1 (MQ=255)
                                                             cTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGa                                                                   >  2:480976/1‑139 (MQ=255)
                                                                cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              >  1:1438154/1‑77 (MQ=255)
                                                                cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:1438154/77‑1 (MQ=255)
                                                                cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                      <  1:300757/133‑1 (MQ=255)
                                                                cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                               <  2:998876/75‑5 (MQ=255)
                                                                cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                               >  1:998876/1‑71 (MQ=255)
                                                                cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAAAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                      >  2:300757/1‑133 (MQ=255)
                                                                 acaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              >  1:1688252/1‑76 (MQ=255)
                                                                 acaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:1688252/76‑1 (MQ=255)
                                                                  caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              <  1:480976/139‑1 (MQ=255)
                                                                     aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:1308533/72‑1 (MQ=255)
                                                                     aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              >  1:1308533/1‑72 (MQ=255)
                                                                          gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTaa                                                      <  2:797239/139‑1 (MQ=255)
                                                                               aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAAc                                                    >  2:1175039/1‑136 (MQ=255)
                                                                               aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAAc                                                    <  1:1175039/136‑1 (MQ=255)
                                                                               aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              >  2:1433913/1‑56 (MQ=255)
                                                                               aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaaggac                                                                                                                              <  1:1433913/61‑6 (MQ=255)
                                                                                          aacaacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCTCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGttt                                      <  2:1065348/139‑1 (MQ=255)
                                                                                          aacaacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGttt                                      <  2:728755/139‑1 (MQ=255)
                                                                                            caacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:1050342/49‑1 (MQ=39)
                                                                                            caacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              >  1:1050342/1‑49 (MQ=39)
                                                                                            caacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa                                    >  1:120255/1‑139 (MQ=255)
                                                                                             aacCTGCGCCGAAGAGCAGGTAAAGCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACGTTCCTGTGTAAACGTTACCAATTGTTTaag                                   <  2:1628047/139‑1 (MQ=255)
                                                                                              acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaaga                                  <  2:120255/139‑1 (MQ=255)
                                                                                              acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaaga                                  <  1:1161900/139‑1 (MQ=255)
                                                                                              acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaaga                                  <  2:1400578/139‑1 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtat                             >  2:458710/1‑139 (MQ=255)
                                                                                                                        ttACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt        <  1:458710/139‑1 (MQ=255)
                                                                                                                          aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                              >  1:1027023/1‑51 (MQ=255)
                                                                                                                          aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                              <  2:1027023/51‑1 (MQ=255)
                                                                                                                          aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                                                         <  1:894794/56‑1 (MQ=255)
                                                                                                                          aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                                                         >  2:894794/1‑56 (MQ=255)
                                                                                                                          aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATaa      >  1:1237301/1‑139 (MQ=255)
                                                                                                                             atccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCa                                             <  2:1235084/93‑1 (MQ=255)
                                                                                                                              aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCa                                             >  1:1235084/1‑96 (MQ=255)
                                                                                                                              aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc  >  2:684730/1‑139 (MQ=255)
                                                                                                                              aTCCCCAAAAGGACACTGTTAATGAAGGAGAAAAAATCTGACATGCATATCCCTCTTATTGCCGGTCGCGATAACTTTCCTGTGTAAACGTTACCAATTCTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc  >  1:1029786/1‑139 (MQ=255)
                                                                                                                               ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                      >  2:1689710/2‑69 (MQ=255)
                                                                                                                               ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                      <  1:1689710/68‑1 (MQ=255)
                                                                                                                               ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa                                    <  2:1421222/102‑1 (MQ=255)
                                                                                                                               ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa                                    >  1:1421222/2‑103 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTC  >  NC_000913/7791‑8054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: