Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,767,438 | 1 | . | C | 88.6% | 126.9 / 9.0 | 44 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Reads supporting (aligned to +/- strand): ref base . (4/1); new base C (16/23); total (20/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.60e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAA > NC_000913/2767301‑2767567 | aGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c > 1:1121746/1‑139 (MQ=255) ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c < 2:1680269/139‑1 (MQ=255) tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c‑cc > 2:117592/1‑139 (MQ=255) ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑a‑c‑c‑cac > 1:1200677/1‑137 (MQ=255) tctcCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAAGCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACaa > 1:1523338/1‑139 (MQ=255) ctcCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAAc < 1:446927/139‑1 (MQ=255) aCGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCTTGc < 2:1091115/133‑1 (MQ=255) aCGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGc > 1:1091115/1‑133 (MQ=255) cGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGa < 1:435267/139‑1 (MQ=255) gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c‑cc < 1:1326997/114‑1 (MQ=255) gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c‑cc > 2:1326997/1‑114 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAAGCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAac < 2:1523338/139‑1 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAac < 2:23520/139‑1 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTc > 1:1480645/1‑125 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTc < 2:1480645/125‑1 (MQ=255) aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca > 2:1477594/1‑118 (MQ=255) aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca < 1:1477594/118‑1 (MQ=255) gTCAATGGATTTTTGAATCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca < 2:1648697/112‑1 (MQ=255) gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca > 1:1648697/1‑112 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCtgtg > 1:889685/1‑139 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 2:889685/139‑1 (MQ=255) tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAccc < 1:117592/139‑1 (MQ=255) tcttcctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca > 2:16131/1‑90 (MQ=255) tcttcctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca < 1:16131/90‑1 (MQ=255) tctAAACGAAGCAGGATTCAATTCGGCCTTTTGGCATGATCTTAACTTGTCCAATATGATCCAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGAATAAATTTccc < 2:325200/139‑1 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAGCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAATTccc < 2:1121746/139‑1 (MQ=255) aaCCACTATATAATTCCCCCTTTTTCCATCATTTTAACTTTTCCCCTATTATTCAATCCCCTTCAC‑C‑C‑C‑CCC‑CCCCCCACCTCCTCCATAGGGAACACCTTTTCCCCTGTTCCCCCTCGACTAAATTTCCCCCCCTTTa < 2:1016309/139‑1 (MQ=255) aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca < 1:1431100/77‑1 (MQ=255) aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTcaca > 2:1431100/1‑77 (MQ=255) ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c‑ccc‑cc < 1:493586/60‑2 (MQ=255) ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c‑ccc‑cc > 2:493586/1‑59 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAA‑C‑C‑C‑CCC‑CTCACAACGTCTCGCATAGGGAACACGTTTACCCCTGTACACCCACGCCTAAATTTCCCCCCCTCTACACTTACCACTAATGt > 2:1593884/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTCAACtt > 2:1200/1‑128 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt < 1:1200/128‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c‑ccc‑cctc > 2:614669/1‑56 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c‑ccc‑cctc < 1:614669/59‑4 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑C‑CCCTCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGt > 1:23269/1‑139 (MQ=255) atatTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 1:930363/139‑1 (MQ=255) tatTCACTTGTCCAAAATGATGAAATAGCCTT‑AA‑C‑CTC‑CCC‑CACACATCATACTGCATGTGGAGCACGTTTTCCCCTGTGCTCCCCCGACTAAAGTTCCCCCCCTTTAAACTTGTCAATAATGTCACCAATTTTCTGGa < 2:1200677/139‑1 (MQ=255) gTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTccc < 1:353233/66‑1 (MQ=255) gTCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTccc > 2:353233/1‑66 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCTCAACGt < 2:384616/41‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCa < 2:761871/47‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCa > 1:761871/1‑47 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt < 1:1017832/81‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt > 2:1017832/1‑81 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 1:1116934/128‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg > 2:1116934/1‑128 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatga > 2:784512/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGACACCAATTTTCTGGAAATCCTatga > 2:209359/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AA‑CAC‑C‑CCC‑CTCACAGCGTCTAGCATAGGGAATACGTTTTAACCTATGCACCCACGACTAAATTACACCCCCTTTAAACTCCTCAATAATCTCACCAATTTTCTTAAAAACCTatgc > 1:722848/1‑138 (MQ=255) tCCAATATGATCAAATAGCATT‑AA‑C‑C‑CTCCC‑CTCACAACGt > 1:384616/1‑41 (MQ=255) tatGATCAAATAGCATT‑AA‑C‑C‑C‑CCCTCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCt < 2:23269/139‑1 (MQ=255) tCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAac > 2:468003/1‑45 (MQ=255) tCAAATAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAac < 1:468003/45‑1 (MQ=255) tAGCATT‑AACC‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaa < 1:784512/139‑1 (MQ=255) | AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑C‑CCC‑CTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAA > NC_000913/2767301‑2767567 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |