Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,4381.C88.6% 126.9 / 9.0 44intergenic (+83/‑272)rnlB/yfjPCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base . (4/1);  new base C (16/23);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAA  >  NC_000913/2767301‑2767567
                                                                                                                                           |                                                                                                                                     
aGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAc                                                                                                                                      >  1:1121746/1‑139 (MQ=255)
  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccc                                                                                                                                  <  2:1680269/139‑1 (MQ=255)
    tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccc                                                                                                                               >  2:117592/1‑139 (MQ=255)
     ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAacccac                                                                                                                              >  1:1200677/1‑137 (MQ=255)
             tctcCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAAGCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACaa                                                                                                                      >  1:1523338/1‑139 (MQ=255)
              ctcCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAAc                                                                                                                     <  1:446927/139‑1 (MQ=255)
                           aCGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCTTGc                                                                                                              <  2:1091115/133‑1 (MQ=255)
                           aCGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGc                                                                                                              >  1:1091115/1‑133 (MQ=255)
                            cGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGa                                                                                                       <  1:435267/139‑1 (MQ=255)
                             gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccc                                                                                                                               <  1:1326997/114‑1 (MQ=255)
                             gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccc                                                                                                                               >  2:1326997/1‑114 (MQ=255)
                              ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAAGCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                     <  2:1523338/139‑1 (MQ=255)
                              ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                     <  2:23520/139‑1 (MQ=255)
                               cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                  >  1:1480645/1‑125 (MQ=255)
                               cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                  <  2:1480645/125‑1 (MQ=255)
                                 aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       >  2:1477594/1‑118 (MQ=255)
                                 aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       <  1:1477594/118‑1 (MQ=255)
                                       gTCAATGGATTTTTGAATCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       <  2:1648697/112‑1 (MQ=255)
                                       gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       >  1:1648697/1‑112 (MQ=255)
                                             ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCtgtg                                                                                      >  1:889685/1‑139 (MQ=255)
                                                 ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                  <  2:889685/139‑1 (MQ=255)
                                                  tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAccc                                                                                 <  1:117592/139‑1 (MQ=255)
                                                             tcttcctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       >  2:16131/1‑90 (MQ=255)
                                                             tcttcctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       <  1:16131/90‑1 (MQ=255)
                                                                 tctAAACGAAGCAGGATTCAATTCGGCCTTTTGGCATGATCTTAACTTGTCCAATATGATCCAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGAATAAATTTccc                                                                  <  2:325200/139‑1 (MQ=255)
                                                                 tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAGCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAATTccc                                                                  <  2:1121746/139‑1 (MQ=255)
                                                                         aaCCACTATATAATTCCCCCTTTTTCCATCATTTTAACTTTTCCCCTATTATTCAATCCCCTTCACCCCCCCCCCCCCACCTCCTCCATAGGGAACACCTTTTCCCCTGTTCCCCCTCGACTAAATTTCCCCCCCTTTa                                                          <  2:1016309/139‑1 (MQ=255)
                                                                          aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       <  1:1431100/77‑1 (MQ=255)
                                                                          aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                       >  2:1431100/1‑77 (MQ=255)
                                                                                      ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccccccc                                                                                                                           <  1:493586/60‑2 (MQ=255)
                                                                                      ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccccccc                                                                                                                           >  2:493586/1‑59 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAACCCCCCCTCACAACGTCTCGCATAGGGAACACGTTTACCCCTGTACACCCACGCCTAAATTTCCCCCCCTCTACACTTACCACTAATGt                                          >  2:1593884/1‑139 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTCAACtt                                                     >  2:1200/1‑128 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt                                                     <  1:1200/128‑1 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccccccctc                                                                                                                         >  2:614669/1‑56 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccccccctc                                                                                                                         <  1:614669/59‑4 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCTCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGt                                          >  1:23269/1‑139 (MQ=255)
                                                                                                       atatTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                            <  1:930363/139‑1 (MQ=255)
                                                                                                        tatTCACTTGTCCAAAATGATGAAATAGCCTTAACCTCCCCCACACATCATACTGCATGTGGAGCACGTTTTCCCCTGTGCTCCCCCGACTAAAGTTCCCCCCCTTTAAACTTGTCAATAATGTCACCAATTTTCTGGa                           <  2:1200677/139‑1 (MQ=255)
                                                                                                                 gTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                           <  1:353233/66‑1 (MQ=255)
                                                                                                                 gTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                           >  2:353233/1‑66 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCTCAACGt                                                                                                                   <  2:384616/41‑1 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCa                                                                                                             <  2:761871/47‑1 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCa                                                                                                             >  1:761871/1‑47 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt                                                                           <  1:1017832/81‑1 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt                                                                           >  2:1017832/1‑81 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                            <  1:1116934/128‑1 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                            >  2:1116934/1‑128 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatga                 >  2:784512/1‑139 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGACACCAATTTTCTGGAAATCCTatga                 >  2:209359/1‑139 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACACCCCCCTCACAGCGTCTAGCATAGGGAATACGTTTTAACCTATGCACCCACGACTAAATTACACCCCCTTTAAACTCCTCAATAATCTCACCAATTTTCTTAAAAACCTatgc                 >  1:722848/1‑138 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCTCCCCTCACAACGt                                                                                                                   >  1:384616/1‑41 (MQ=255)
                                                                                                                       tatGATCAAATAGCATTAACCCCCCTCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCt            <  2:23269/139‑1 (MQ=255)
                                                                                                                            tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                     >  2:468003/1‑45 (MQ=255)
                                                                                                                            tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                     <  1:468003/45‑1 (MQ=255)
                                                                                                                                 tAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaa  <  1:784512/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAA  >  NC_000913/2767301‑2767567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: