Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,330,357 +T coding (1395/1434 nt) dacB → D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,330,3531.T100.0% 130.2 / NA 41R464L (CGT→CTT) dacBD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (20/21);  total (20/21)

TGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGT  >  NC_000913/3330224‑3330455
                                                                                                                                  |                                                                                                       
tGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttgaaa                                                                                                >  1:581732/1‑134 (MQ=255)
     ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            <  2:581732/139‑1 (MQ=255)
          atataACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            <  2:618723/134‑1 (MQ=255)
          atataACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            >  1:618723/1‑134 (MQ=255)
             taACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                    <  2:483375/139‑1 (MQ=255)
             taACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                    <  2:433660/139‑1 (MQ=255)
                 cTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGat                                                                                >  1:275267/1‑139 (MQ=255)
                 cTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttga                                                                                                  >  1:1278793/1‑117 (MQ=255)
                 cTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttga                                                                                                  <  2:1278793/120‑4 (MQ=255)
                                     agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            >  1:171764/1‑107 (MQ=255)
                                     agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            <  2:171764/107‑1 (MQ=255)
                                         agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTAt                                                                          >  2:840599/1‑121 (MQ=255)
                                         agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTAt                                                                          <  1:840599/121‑1 (MQ=255)
                                                    aaTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                    <  1:342544/100‑1 (MQ=255)
                                                    aaTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                    >  2:342544/1‑100 (MQ=255)
                                                        gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            <  1:1609831/88‑1 (MQ=255)
                                                        gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            >  2:1609831/1‑88 (MQ=255)
                                                              gtgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            <  1:514601/82‑1 (MQ=255)
                                                              gtgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            >  2:514601/1‑82 (MQ=255)
                                                                      tCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCa                          >  2:293161/1‑139 (MQ=255)
                                                                         ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGta                                                                                       >  1:1407227/1‑76 (MQ=255)
                                                                         ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGta                                                                                       <  2:1407227/76‑1 (MQ=255)
                                                                         ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac‑a                                        <  2:950250/122‑1 (MQ=255)
                                                                         ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac‑a                                        >  1:950250/1‑122 (MQ=255)
                                                                                        aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACaa              <  2:1418910/133‑1 (MQ=255)
                                                                                        aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACaa              >  1:1418910/1‑133 (MQ=255)
                                                                                         gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTgcgc       <  1:293161/139‑1 (MQ=255)
                                                                                          aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt      <  2:275267/139‑1 (MQ=255)
                                                                                            ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt    >  1:1300649/1‑139 (MQ=255)
                                                                                              tGCGGATCAGCGTAAACGCCATATTCCGTTAGTGCGTTTTTGAAACCCATTGGTAAAAGGAAATTTATCAGAACAATAAAT‑AAAAAAAAAACCCAGCACAATGGCCGGGGCAGCAGATAATTAACaaaatcagcttaga  >  2:56998/1‑128 (MQ=255)
                                                                                               gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                       <  1:532642/70‑1 (MQ=255)
                                                                                               gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                       >  2:532642/1‑70 (MQ=255)
                                                                                               gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCg                                  >  1:937484/1‑106 (MQ=255)
                                                                                               gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCg                                  <  2:937484/106‑1 (MQ=255)
                                                                                                 ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGt           <  1:566919/127‑1 (MQ=255)
                                                                                                 ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGt           >  2:566919/1‑127 (MQ=255)
                                                                                                      aGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            <  1:651214/42‑1 (MQ=38)
                                                                                                      aGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            <  2:1004516/42‑1 (MQ=38)
                                                                                                      aGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            >  1:1004516/1‑42 (MQ=255)
                                                                                                      aGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                            >  2:651214/1‑42 (MQ=255)
                                                                                                                  gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                          <  1:1692558/80‑1 (MQ=255)
                                                                                                                  gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                          >  2:1692558/1‑80 (MQ=255)
                                                                                                                     ttCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt     <  1:1415803/113‑1 (MQ=255)
                                                                                                                     ttCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt     >  2:1415803/1‑113 (MQ=255)
                                                                                                                                  |                                                                                                       
TGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGT  >  NC_000913/3330224‑3330455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑