Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,757,753	Δ5 bp	coding (969‑973/1005 nt)	ldtE ←	murein L,D‑transpeptidase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1757753	1757757	5	55 [0]	[0] 57	ldtE	murein L,D‑transpeptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1757752	0 (0.000)	55 (0.870)	37/200	0.5	100%	coding (974/1005 nt)	ldtE	murein L,D‑transpeptidase
	?	NC_000913	1757758 =	0 (0.000)					coding (968/1005 nt)	ldtE	murein L,D‑transpeptidase

ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1757617‑1757752 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTT  >  NC_000913/1757758‑1757893                                                                                                                                                                                                                                                                                  ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCA                                                                                                                                       <  2:741964/139‑1                 CATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACT                                                                                                                       <  2:885158/139‑1                           AAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGG                                                                                                             >  2:1649823/1‑139                                              ACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGA                                                                                          >  1:872498/1‑139                                                CTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGT                                                                                        >  1:117593/1‑139                                                CTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGT                                                                                        <  1:1649823/139‑1                                                         GAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           <  2:1249957/127‑1                                                         GAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           >  1:1249957/1‑127                                                                    CACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           <  1:402316/116‑1                                                                    CACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           >  2:402316/1‑116                                                                    CACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCT                                                                                                    >  2:744839/1‑107                                                                    CACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCT                                                                                                    <  1:744839/107‑1                                                                         TGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGA                                                               >  1:633197/1‑139                                                                           TGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATA                                                             >  1:1648070/1‑139                                                                           TGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCAC                                                                         <  1:613149/127‑1                                                                           TGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCAC                                                                         >  2:613149/1‑127                                                                            GCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATAC                                                            >  1:100381/1‑139                                                                             CGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACC                                                           >  2:388760/1‑139                                                                                CATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG                                                        <  2:1648070/139‑1                                                                                     TTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGA                                                   <  2:633197/139‑1                                                                                     TTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGA                                                   >  1:194184/1‑139                                                                                             TGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAA                                           >  2:805181/1‑139                                                                                             TGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           >  2:870903/1‑91                                                                                             TGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           <  1:870903/91‑1                                                                                             TGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTG                                                                                                                 >  2:217558/1‑69                                                                                             TGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTG                                                                                                                 <  1:217558/69‑1                                                                                                            CTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAG                                                                                                              <  2:935377/57‑1                                                                                                            CTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAG                                                                                                              >  1:935377/1‑57                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACT                              <  1:198093/134‑1                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACT                              >  2:198093/1‑134                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCA                                                                                 >  1:505473/1‑83                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCA                                                                                 <  2:505473/83‑1                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           <  2:1055346/73‑1                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                           >  1:1055346/1‑73                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGC                                                                                                                              >  2:554947/1‑38                                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGC                                                                                                                              <  1:554947/38‑1                                                                                                                 TCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG                                                        >  1:130895/1‑106                                                                                                                 TCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG                                                        <  2:130895/106‑1                                                                                                                    CGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTAT                                   >  2:774394/1‑124                                                                                                                    CGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTAT                                   <  1:774394/124‑1                                                                                                                    CGCGTAACATATTCCCTTGCTTCACCATTCTGCGCT                                                                                                                           >  2:850529/1‑36                                                                                                                    CGCGTAACATATTCCCTTGCTTCACCATTCTGCGCT                                                                                                                           <  1:850529/36‑1                                                                                                                    CGCGTAACATATTCCCTTGCTTCACCATTCTGCGC                                                                                                                            >  1:1213525/1‑35                                                                                                                    CGCGTAACATATTCCCTTGCTTCACCATTCTGCGC                                                                                                                            <  2:1213525/35‑1                                                                                                                     GCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATC                   <  2:872498/139‑1                                                                                                                               TTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCAC                                                                         <  2:1484301/75‑1                                                                                                                               TTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCAC                                                                         >  1:1484301/1‑75                                                                                                                                 CCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATT       >  1:425107/1‑139                                                                                                                                 CCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATT       >  2:1061668/1‑139                                                                                                                                   CTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTACCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGT     >  1:1522617/1‑139                                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCT   >  1:1007739/1‑139                                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCT   >  2:1187188/1‑139                                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG                                                        >  1:420572/1‑86                                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG                                                        <  2:420572/86‑1                                                                                                                                      GCTTCACCATTCTGCGCTGACTCTGCTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTT  >  1:845299/1‑139                                                                                                                                                                                                                                                                                  ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1757617‑1757752 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTT  >  NC_000913/1757758‑1757893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.