Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	544,754	C→T	R180H (CGT→CAT)	allC ←	allantoate amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	544,754	0	C	T	100.0%	188.2 / NA	58	R180H (CGT→CAT)	allC	allantoate amidohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

GTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAG  >  NC_000913/544618‑544888                                                                                                                                         |                                                                                                                                       gTTTGATTCGCCGGTCAGCGTTACCGTATACCGAAGCTGCCCGACACTTGTATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTATTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAtgc                                                                                                                                      >  2:1256363/1‑139 (MQ=255)                  gCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGtt                                                                                                                     <  1:768334/139‑1 (MQ=255)                                  acgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTCGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                     >  2:1077300/1‑139 (MQ=255)                                                  gCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcat                                                                                     >  1:495884/1‑139 (MQ=255)                                                  gCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcat                                                                                     >  1:944566/1‑139 (MQ=255)                                                       caccacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACa                                                                                <  1:3773/139‑1 (MQ=255)                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTg                                                                                                                            <  1:199907/89‑1 (MQ=255)                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTg                                                                                                                            >  2:199907/1‑89 (MQ=255)                                                                  ttgattgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgc                                                                                       >  1:1463069/1‑121 (MQ=255)                                                                  ttgattgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgc                                                                                       <  2:1463069/121‑1 (MQ=255)                                                                       tgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCgg                                                                                                                  >  2:102986/1‑89 (MQ=255)                                                                       tgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCgg                                                                                                                  <  1:102986/89‑1 (MQ=255)                                                                           cATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATc                                                            <  1:1567619/139‑1 (MQ=255)                                                                              tACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATcaca                                                         <  1:885498/139‑1 (MQ=255)                                                                                      aGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTggg                                                                                                                          >  1:936552/1‑66 (MQ=255)                                                                                      aGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTggg                                                                                                                          <  2:936552/66‑1 (MQ=255)                                                                                        cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGc                                                                                                    <  1:1391048/86‑1 (MQ=255)                                                                                        cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGc                                                                                                    >  2:1391048/1‑86 (MQ=255)                                                                                          cacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTcc                                                                     <  2:1148924/115‑1 (MQ=255)                                                                                          cacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTcc                                                                     >  1:1148924/1‑115 (MQ=255)                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAtgc                                                                                                                                      <  2:1267686/47‑1 (MQ=255)                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAtgc                                                                                                                                      >  1:1267686/1‑47 (MQ=255)                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcgt                                        <  2:944566/139‑1 (MQ=255)                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcgt                                        <  2:389161/139‑1 (MQ=255)                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcgt                                        >  1:389161/1‑139 (MQ=255)                                                                                                  tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTg                                                                 <  2:575643/111‑1 (MQ=255)                                                                                                  tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTg                                                                 >  1:575643/1‑111 (MQ=255)                                                                                                  tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAg                                     >  2:474293/1‑139 (MQ=255)                                                                                                  tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAg                                     >  1:1191311/1‑139 (MQ=255)                                                                                                    ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTa                                                                          <  1:508707/100‑1 (MQ=255)                                                                                                    ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTa                                                                          >  2:508707/1‑100 (MQ=255)                                                                                                       aaTATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgca                                                                                      >  2:1543803/1‑85 (MQ=255)                                                                                                       aaTATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgca                                                                                      <  1:1543803/85‑1 (MQ=255)                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc                              <  2:495884/139‑1 (MQ=255)                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc                              <  2:1191311/139‑1 (MQ=255)                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCACCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc                              <  1:1256363/139‑1 (MQ=255)                                                                                                          atGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGT‑GGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                                >  1:254137/1‑103 (MQ=255)                                                                                                          atGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGT‑GGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                                <  2:254137/103‑1 (MQ=255)                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                     >  1:1139196/1‑63 (MQ=255)                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                     <  2:1139196/63‑1 (MQ=255)                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                         >  2:1142701/1‑139 (MQ=255)                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                         >  1:664849/1‑139 (MQ=255)                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                         >  1:1239134/1‑139 (MQ=255)                                                                                                                ttCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcat                                                                                     >  1:318957/1‑77 (MQ=255)                                                                                                                ttCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcat                                                                                     <  2:318957/77‑1 (MQ=255)                                                                                                                ttCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCa                       >  2:1190534/1‑139 (MQ=255)                                                                                                                ttCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACG‑CGTCAGGATTCGCCAGCCCaa                      >  1:225685/1‑139 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTaa                                                                                                          >  2:147565/1‑46 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTaa                                                                                                          <  1:147565/46‑1 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                     >  1:1220849/1‑51 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                     <  2:1220849/51‑1 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                             >  2:1112122/1‑91 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                             <  1:1112122/91‑1 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcgt                                        >  2:1686776/1‑112 (MQ=255)                                                                                                                          gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcgt                                        <  1:1686776/112‑1 (MQ=255)                                                                                                                             ttAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc          <  1:1190534/139‑1 (MQ=255)                                                                                                                               aaTATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg        <  2:664849/139‑1 (MQ=255)                                                                                                                                    ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa   >  1:535570/1‑139 (MQ=255)                                                                                                                                     cTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaa                     >  2:1478554/1‑120 (MQ=255)                                                                                                                                     cTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaa                     <  1:1478554/120‑1 (MQ=255)                                                                                                                                     cTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATAGCATCGACAAAACTATTTCCTTTTGCATCACAGATATTCTGCACGTCGTCCGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag  <  1:1077300/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAG  >  NC_000913/544618‑544888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑