Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	607,988	G→A	*51* (TAG→TAA)	hokE →	toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	607,988	0	G	A	100.0%	150.3 / NA	47	*51* (TAG→TAA)	hokE	toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

TGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCC  >  NC_000913/607856‑608118                                                                                                                                     |                                                                                                                                   tGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGAGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGt                                                                                                                                >  2:516864/1‑137 (MQ=255) tGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGAGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGt                                                                                                                                <  1:516864/137‑1 (MQ=255)  gCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtgt                                                                                                                             >  1:1176000/1‑139 (MQ=255)     gCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                                          <  1:1106489/139‑1 (MQ=255)         tCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAGCCGAAGAAGTAACCGTTGTGCGGGGa                                                                                                                      <  2:1600062/139‑1 (MQ=255)                    tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCAt                                                                                                            <  2:432108/138‑1 (MQ=255)                    tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCAt                                                                                                            >  1:432108/1‑138 (MQ=255)                          aCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                     <  2:1176000/139‑1 (MQ=255)                                cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGGACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                               <  1:560780/139‑1 (MQ=255)                                        tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                                    <  1:174414/110‑1 (MQ=255)                                        tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                                    >  2:174414/1‑110 (MQ=255)                                        tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgt                                                                                       >  2:165055/1‑139 (MQ=255)                                             ttCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                                  <  1:165055/139‑1 (MQ=255)                                               cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTa                                                                                >  2:14316/1‑139 (MQ=255)                                                       agaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                                                                   >  1:386995/1‑128 (MQ=255)                                                       agaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                                                                   <  2:386995/128‑1 (MQ=255)                                                                   tgAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc                                                                             >  2:1098979/1‑122 (MQ=255)                                                                   tgAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc                                                                             <  1:1098979/122‑1 (MQ=255)                                                                           cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAATGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGa                                                    >  2:1524547/1‑139 (MQ=255)                                                                           cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGa                                                    >  1:499226/1‑139 (MQ=255)                                                                             gTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                                                  >  2:1574744/1‑139 (MQ=255)                                                                                aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAc                                               >  1:889179/1‑139 (MQ=255)                                                                                 aGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                              >  1:550883/1‑139 (MQ=255)                                                                                 aGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                              <  1:14316/139‑1 (MQ=255)                                                                                        tAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                                    >  1:550475/1‑62 (MQ=255)                                                                                        tAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                                    <  2:550475/62‑1 (MQ=255)                                                                                                aGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGt                               >  2:56500/1‑139 (MQ=255)                                                                                                  tttAAGGCTGTTCTCGCTTACGAACCAAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCa                             >  2:1401787/1‑139 (MQ=255)                                                                                                   ttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAATGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                            <  1:1524547/139‑1 (MQ=255)                                                                                                    tAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                                        >  2:811182/1‑46 (MQ=255)                                                                                                    tAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                                        <  1:811182/46‑1 (MQ=255)                                                                                                        gCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                       <  1:69403/139‑1 (MQ=255)                                                                                                          tGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                                       >  1:563481/1‑57 (MQ=255)                                                                                                          tGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                                       <  2:563481/57‑1 (MQ=255)                                                                                                          tGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                           >  2:148846/1‑69 (MQ=255)                                                                                                          tGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                           <  1:808454/69‑1 (MQ=255)                                                                                                          tGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                           >  2:808454/1‑69 (MQ=255)                                                                                                          tGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                           <  1:148846/69‑1 (MQ=255)                                                                                                            ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                                                         <  1:554409/85‑1 (MQ=255)                                                                                                            ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                                                         >  2:554409/1‑85 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                  <  1:56500/139‑1 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                  <  2:499226/139‑1 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                  <  1:1574744/139‑1 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                  <  2:889179/139‑1 (MQ=255)                                                                                                                      cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                     >  2:1085657/1‑47 (MQ=255)                                                                                                                      cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                     <  1:1085657/47‑1 (MQ=255)                                                                                                                             aagaagTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGccc  >  1:181863/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                   TGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCC  >  NC_000913/607856‑608118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑