Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	671,676	C→T	E171K (GAA→AAA)	lptE ←	LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	671,676	0	C	T	100.0%	160.4 / NA	50	E171K (GAA→AAA)	lptE	LPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/26);  total (24/26)

AAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCG  >  NC_000913/671540‑671806                                                                                                                                         |                                                                                                                                   aaGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTttgt                                                                                                                                  >  1:1499802/1‑139 (MQ=255)      aCGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGaa                                                                                                                             >  1:1295575/1‑139 (MQ=255)       cgccgcGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGaa                                                                                                                             >  1:753896/1‑138 (MQ=255)       cgccgcGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGaa                                                                                                                             <  2:753896/138‑1 (MQ=255)        gccgcGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACg                                                                                                                           >  2:1050739/1‑139 (MQ=255)        gccgcGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACg                                                                                                                           >  1:1442280/1‑139 (MQ=255)           gcgcgtAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAat                                                                                                                        <  1:1050739/139‑1 (MQ=255)           gcgcgcAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAat                                                                                                                        >  2:477311/1‑139 (MQ=255)            cgcgcAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAata                                                                                                                       >  1:845183/1‑139 (MQ=255)              cgcAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATc                                                                                                                     <  2:1189898/139‑1 (MQ=255)                 aGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCgc                                                                                                                  <  2:1499802/139‑1 (MQ=255)                     cTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCgcagca                                                                                                              <  2:454523/139‑1 (MQ=255)                                tGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTgg                                                                                                   <  1:477311/139‑1 (MQ=255)                                 gcgcgcGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGTCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGc                                                                                                  <  1:198761/139‑1 (MQ=255)                                  cgcgcgGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCgg                                                                                                                               <  1:270193/109‑1 (MQ=255)                                  cgcgcgGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCgg                                                                                                                               >  2:270193/1‑109 (MQ=255)                                  cgcgcgGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCa                                                                                                 >  1:376352/1‑139 (MQ=255)                                  cgcgcgGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCa                                                                                                 >  1:705006/1‑139 (MQ=255)                                  cgcgcgGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCa                                                                                                 >  1:319587/1‑139 (MQ=255)                                     gcgGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCt                                                                                              <  1:874557/139‑1 (MQ=255)                                          gttgttCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGa                                                                                         <  2:1442280/139‑1 (MQ=255)                                               tCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAg                                                                                    <  2:1295575/139‑1 (MQ=255)                                                   ggTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTa                                                                                            <  2:1406745/127‑1 (MQ=255)                                                   ggTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTa                                                                                            >  1:1406745/1‑127 (MQ=255)                                                     tACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTc                                                                              >  1:390389/1‑139 (MQ=255)                                                            gAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       <  2:319587/139‑1 (MQ=255)                                                             aaTCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       <  2:139953/138‑1 (MQ=255)                                                             aaTCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       >  1:139953/1‑138 (MQ=255)                                                                        aCCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACAtc                                                           >  1:1441277/1‑139 (MQ=255)                                                                                tggtggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTa                                                    >  1:827804/1‑138 (MQ=255)                                                                                tggtggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTa                                                    <  2:827804/138‑1 (MQ=255)                                                                                  gtggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGa                                                 <  2:390389/139‑1 (MQ=255)                                                                                   tggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGaa                                                                                                                             <  1:619588/62‑1 (MQ=255)                                                                                   tggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGaa                                                                                                                             >  2:619588/1‑62 (MQ=255)                                                                                   tggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       >  1:456017/1‑116 (MQ=255)                                                                                   tggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       <  2:456017/116‑1 (MQ=255)                                                                                    ggAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATc                                               <  2:1441277/139‑1 (MQ=255)                                                                                        aCGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATc                                                                                                                     >  2:1407866/1‑65 (MQ=255)                                                                                        aCGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATc                                                                                                                     <  1:1407866/65‑1 (MQ=255)                                                                                             tGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACAt                                                            >  2:808628/1‑117 (MQ=255)                                                                                             tGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACAt                                                            <  1:808628/117‑1 (MQ=255)                                                                                             tGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACg                                                  <  1:910559/127‑1 (MQ=255)                                                                                             tGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACg                                                  >  2:910559/1‑127 (MQ=255)                                                                                                            aGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       <  2:96306/91‑1 (MQ=255)                                                                                                            aGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       <  2:687492/91‑1 (MQ=255)                                                                                                            aGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       <  2:644047/91‑1 (MQ=255)                                                                                                            aGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       >  1:96306/1‑91 (MQ=255)                                                                                                            aGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       >  1:687492/1‑91 (MQ=255)                                                                                                            aGTATCCGTTGTGGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGgcagca                                                                       >  1:644047/1‑91 (MQ=255)                                                                                                                       tgGTCGACGTCTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTg            <  2:376352/139‑1 (MQ=255)                                                                                                                                 cTGTTCTTTGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCg  >  1:1145656/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                   AAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCG  >  NC_000913/671540‑671806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑