Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 69,623 G→C D142E (GAC→GAG araB ← L‑ribulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091369,6230GC100.0% 169.9 / NA 48D142E (GAC→GAGaraBL‑ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/24);  total (24/24)

CAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCA  >  NC_000913/69488‑69747
                                                                                                                                       |                                                                                                                            
cAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCa                                                                                                                           >  2:1509202/1‑139 (MQ=255)
                       gCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACaa                                                                                                    <  1:817114/139‑1 (MQ=255)
                       gCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACaa                                                                                                    <  1:1509202/139‑1 (MQ=255)
                            aTCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACggg                                                                                               >  1:813768/1‑139 (MQ=255)
                                     gCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATctct                                                                                       >  2:1576296/1‑138 (MQ=255)
                                     gCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATctct                                                                                       <  1:1576296/138‑1 (MQ=255)
                                     gCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCtt                                                                                      >  1:245770/1‑139 (MQ=255)
                                        gCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCg                                                                                   <  2:245770/139‑1 (MQ=255)
                                            aTTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGcttc                                                                               >  1:247048/1‑139 (MQ=255)
                                                  ccACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAAc                                                                         <  1:1439347/139‑1 (MQ=255)
                                                          cTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAgtgt                                                                 <  2:1612547/139‑1 (MQ=255)
                                                                     aGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCcac                                                      <  2:813768/139‑1 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCgg                                                                                                                <  1:3877/79‑1 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCgg                                                                                                                >  2:3877/1‑79 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGca                                                                                                       <  1:1630536/88‑1 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGca                                                                                                       <  1:17200/88‑1 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGca                                                                                                       >  2:1630536/1‑88 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGca                                                                                                       >  2:17200/1‑88 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTa                                                                                             <  1:530339/98‑1 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTa                                                                                             >  2:530339/1‑98 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCcac                                                      >  1:1453218/1‑137 (MQ=255)
                                                                       tCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCcac                                                      <  2:1453218/137‑1 (MQ=255)
                                                                                      ttGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGtt                                     >  2:1620918/1‑139 (MQ=255)
                                                                                           cAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGt                                <  2:247048/139‑1 (MQ=255)
                                                                                              aaCCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCa                                                                                                                           <  1:906524/45‑1 (MQ=255)
                                                                                              aaCCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCa                                                                                                                           >  2:906524/1‑45 (MQ=255)
                                                                                                   ttCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCg                                                                       <  2:1262667/92‑1 (MQ=255)
                                                                                                   ttCGCTGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCg                                                                       >  1:1262667/1‑92 (MQ=255)
                                                                                                        tGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCt                                                            <  1:778107/98‑1 (MQ=255)
                                                                                                        tGGAATAAATACCACCAATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCt                                                            >  2:778107/1‑98 (MQ=255)
                                                                                                                    caccaATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAgcgc       >  2:1161051/1‑139 (MQ=255)
                                                                                                                      ccaATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGcaca                                                                                                     <  2:79795/43‑1 (MQ=255)
                                                                                                                      ccaATGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGcaca                                                                                                     >  1:79795/1‑43 (MQ=255)
                                                                                                                         aTGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAAc                                                                         >  2:55125/1‑68 (MQ=255)
                                                                                                                         aTGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAAc                                                                         <  1:55125/68‑1 (MQ=255)
                                                                                                                         aTGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTcc                                                        <  1:999482/85‑1 (MQ=255)
                                                                                                                         aTGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCa  >  1:1117517/1‑139 (MQ=255)
                                                                                                                         aTGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTATcc                                                        >  2:999482/1‑85 (MQ=255)
                                                                                                                          tGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAgtgt                                                                 >  1:89286/1‑75 (MQ=255)
                                                                                                                          tGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAgtgt                                                                 <  2:89286/75‑1 (MQ=255)
                                                                                                                          tGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCt                                                            >  1:626273/1‑80 (MQ=255)
                                                                                                                          tGTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCt                                                            <  2:626273/80‑1 (MQ=255)
                                                                                                                           gTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCaa                                                                          >  2:21794/1‑65 (MQ=255)
                                                                                                                           gTAGCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCaa                                                                          <  1:21794/65‑1 (MQ=255)
                                                                                                                              gCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGct                                                                                 <  2:284533/55‑1 (MQ=255)
                                                                                                                              gCGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGct                                                                                 >  1:284533/1‑55 (MQ=255)
                                                                                                                               cGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGgcg             >  1:1539151/1‑122 (MQ=255)
                                                                                                                               cGGGAGTACTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGgcg             <  2:1539151/122‑1 (MQ=255)
                                                                                                                                   agtactCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTc                                    >  2:1279368/1‑95 (MQ=255)
                                                                                                                                   agtactCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTc                                    <  1:1279368/95‑1 (MQ=255)
                                                                                                                                       |                                                                                                                            
CAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCA  >  NC_000913/69488‑69747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: