Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	879,857	G→A	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI → gstB ←	soluble aldose sugar dehydrogenase glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	879,857	0	G	A	100.0%	209.3 / NA	64	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI gstB	soluble aldose sugar dehydrogenase glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/32);  total (32/32)

AGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879728‑879987                                                                                                                                  |                                                                                                                                   agaagaTGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                           <  1:1025259/139‑1 (MQ=255)                        aCAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGc                                                                                                    >  2:32707/1‑139 (MQ=255)                            agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACgg                                                                                                                             >  1:746072/1‑110 (MQ=255)                            agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACgg                                                                                                                             <  2:746072/110‑1 (MQ=255)                            agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                >  2:891357/1‑139 (MQ=255)                              ggCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCg                                                                                              >  2:1335700/1‑139 (MQ=255)                                    gAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                           >  2:968513/1‑104 (MQ=255)                                    gAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                           <  1:968513/104‑1 (MQ=255)                                    gAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                        <  1:891357/139‑1 (MQ=255)                                    gAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                        <  2:1169757/139‑1 (MQ=255)                                       ttCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAg                                                                                     <  1:569201/139‑1 (MQ=255)                                          gTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTg                                                                                  <  1:1335700/139‑1 (MQ=255)                                                ttCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATa                                                                            >  2:1070286/1‑139 (MQ=255)                                                  cGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAat                                                                                                                         >  2:15770/1‑92 (MQ=255)                                                  cGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAat                                                                                                                         <  1:15770/92‑1 (MQ=255)                                                               aCGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          <  1:1394071/94‑1 (MQ=255)                                                               aCGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          >  2:1394071/1‑94 (MQ=255)                                                                     atttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcg                                                       <  1:1070286/139‑1 (MQ=255)                                                                       ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:32707/139‑1 (MQ=255)                                                                             gTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCggg                                                                                                 <  1:1554816/89‑1 (MQ=255)                                                                             gTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCggg                                                                                                 >  2:1554816/1‑89 (MQ=255)                                                                             gTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTcc                                               >  1:98064/1‑139 (MQ=255)                                                                               tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCg                                                                                              <  2:525795/90‑1 (MQ=255)                                                                               tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCg                                                                                              >  1:525795/1‑90 (MQ=255)                                                                               tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:130962/131‑1 (MQ=255)                                                                               tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  2:130962/1‑131 (MQ=255)                                                                                ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAgg                                            >  1:1642476/1‑139 (MQ=255)                                                                                 tcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           >  2:947519/1‑139 (MQ=255)                                                                                       acgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          >  1:786128/1‑70 (MQ=255)                                                                                       acgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          <  2:786128/70‑1 (MQ=255)                                                                                       acgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:1518383/123‑1 (MQ=255)                                                                                       acgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  2:1518383/1‑123 (MQ=255)                                                                                        cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAat                                                                                                                         <  2:209924/54‑1 (MQ=255)                                                                                        cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAat                                                                                                                         >  1:209924/1‑54 (MQ=255)                                                                                        cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGAt                                                                             <  2:861263/98‑1 (MQ=255)                                                                                        cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGAt                                                                             >  1:861263/1‑98 (MQ=255)                                                                                            tCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCa                                                                         <  1:576444/98‑1 (MQ=255)                                                                                            tCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCa                                                                         >  2:576444/1‑98 (MQ=255)                                                                                                 tGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAg                                                                        >  2:837265/1‑94 (MQ=255)                                                                                                 tGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAg                                                                        <  1:837265/94‑1 (MQ=255)                                                                                                 tGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAAc                           >  1:1503508/1‑139 (MQ=255)                                                                                                  ggggAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACa                          <  2:1642476/139‑1 (MQ=255)                                                                                                           cTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAAc                           >  1:511400/1‑129 (MQ=255)                                                                                                           cTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAAc                           <  2:511400/129‑1 (MQ=255)                                                                                                             tAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACt                                                                                                      <  1:871460/52‑1 (MQ=255)                                                                                                             tAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACt                                                                                                      >  2:871460/1‑52 (MQ=255)                                                                                                               aaGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTgg                                                          <  2:1342524/94‑1 (MQ=255)                                                                                                               aaGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTgg                                                          >  1:1342524/1‑94 (MQ=255)                                                                                                                aGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          <  2:625894/45‑1 (MQ=255)                                                                                                                aGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          >  1:625894/1‑45 (MQ=255)                                                                                                                aGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAAc            <  2:98064/139‑1 (MQ=255)                                                                                                                    aGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGaa             <  1:1035590/134‑1 (MQ=255)                                                                                                                    aGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGaa             >  2:1035590/1‑134 (MQ=255)                                                                                                                    aGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTg        >  2:344553/1‑139 (MQ=255)                                                                                                                     gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  2:205702/1‑93 (MQ=255)                                                                                                                     gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:984195/1‑93 (MQ=255)                                                                                                                     gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:205702/93‑1 (MQ=255)                                                                                                                     gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:984195/93‑1 (MQ=255)                                                                                                                     gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGc                                      >  2:1371281/1‑108 (MQ=255)                                                                                                                     gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGc                                      <  1:1371281/108‑1 (MQ=255)                                                                                                                      cccACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcg                                                       <  2:1459516/90‑1 (MQ=255)                                                                                                                      cccACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcg                                                       >  1:1459516/1‑90 (MQ=255)                                                                                                                          cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatag  >  1:1503766/1‑139 (MQ=255)                                                                                                                               ttAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATAcc                                                                          <  2:398156/62‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   AGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879728‑879987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑