Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	892,869	G→A	*301* (TAG→TAA)	rimK →	ribosomal protein S6 modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	892,869	0	G	A	100.0%	233.7 / NA	71	*301* (TAG→TAA)	rimK	ribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/38);  total (33/38)

TTGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAATG  >  NC_000913/892735‑893000                                                                                                                                       |                                                                                                                                    ttGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                 <  2:25045/139‑1 (MQ=255)    gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              <  2:1539581/139‑1 (MQ=255)       aTGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                               >  1:1021799/1‑135 (MQ=255)       aTGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                               <  2:1021799/135‑1 (MQ=255)       aTGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATcac                                                                                                                           >  1:357655/1‑139 (MQ=255)                    cGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACt                                                                                                                     >  2:1313571/1‑132 (MQ=255)                    cGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACt                                                                                                                     <  1:1313571/132‑1 (MQ=255)                       cGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGtt                                                                                                           >  2:1153698/1‑139 (MQ=255)                       cGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGtt                                                                                                           >  2:1018160/1‑139 (MQ=255)                              gCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                    <  1:575753/139‑1 (MQ=255)                                             aaaaaCCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAg                                                                                     <  2:134172/139‑1 (MQ=255)                                                 accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCtgt                                                                                 <  2:357655/139‑1 (MQ=255)                                                     ccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCg                                                                             <  1:1018160/139‑1 (MQ=255)                                                              aCATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTa                                                                    <  1:1153698/139‑1 (MQ=255)                                                                aTCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATc                                                                  >  2:1429946/1‑139 (MQ=255)                                                                  cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              <  1:1610526/77‑1 (MQ=255)                                                                  cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              >  2:1610526/1‑77 (MQ=255)                                                                                 ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              <  1:85913/62‑1 (MQ=255)                                                                                 ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              <  1:769907/62‑1 (MQ=255)                                                                                 ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              >  2:85913/1‑62 (MQ=255)                                                                                 ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              >  2:769907/1‑62 (MQ=255)                                                                                 ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                          <  2:465566/130‑1 (MQ=255)                                                                                 ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                          >  1:465566/1‑130 (MQ=255)                                                                                    cTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              >  1:355456/1‑59 (MQ=255)                                                                                    cTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              <  2:355456/59‑1 (MQ=255)                                                                                       gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              >  2:1045144/1‑56 (MQ=255)                                                                                       gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              <  1:1045144/56‑1 (MQ=255)                                                                                       gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                 >  2:1627683/1‑69 (MQ=255)                                                                                       gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                 <  1:1627683/69‑1 (MQ=255)                                                                                        aTCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGa                                          >  2:80807/1‑139 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              >  2:315568/1‑51 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                              <  1:315568/51‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                       <  1:852547/58‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                       >  2:852547/1‑58 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                       <  2:380406/58‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                       >  1:380406/1‑58 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGc                                                                                          <  2:1452389/87‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGc                                                                                          <  1:758359/87‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGc                                                                                          >  1:1452389/1‑87 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGc                                                                                          >  2:758359/1‑87 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                              >  1:798619/1‑99 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                              <  2:798619/99‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATc                                      >  2:1566143/1‑139 (MQ=255)                                                                                                 cACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAag                                                             <  2:160320/111‑1 (MQ=255)                                                                                                 cACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAag                                                             >  1:160320/1‑111 (MQ=255)                                                                                                 cACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                          <  2:103704/114‑1 (MQ=255)                                                                                                 cACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                          >  1:103704/1‑114 (MQ=255)                                                                                                  aCGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                                <  1:1284098/139‑1 (MQ=255)                                                                                                   cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTAGCGTAAGCTGTGCCGGTCTTTTTATCGaa                                                               >  1:1043851/1‑107 (MQ=255)                                                                                                   cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGACCATGGTTTTACCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGaa                                                               <  2:1043851/107‑1 (MQ=255)                                                                                                        cGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGa                                          >  1:764381/1‑123 (MQ=255)                                                                                                        cGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGa                                          <  2:764381/123‑1 (MQ=255)                                                                                                          aCAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                              <  2:488746/85‑1 (MQ=255)                                                                                                          aCAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                              >  1:488746/1‑85 (MQ=255)                                                                                                              aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                              <  2:1218432/81‑1 (MQ=255)                                                                                                              aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                              >  1:1218432/1‑81 (MQ=255)                                                                                                                    gCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATAc              <  1:80807/139‑1 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                    >  2:85531/1‑52 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                    <  1:85531/52‑1 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTAt                                                                   >  1:507895/1‑85 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTAt                                                                   <  2:507895/85‑1 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                                <  1:1115650/120‑1 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                                >  2:1115650/1‑120 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACg             <  2:131477/139‑1 (MQ=255)                                                                                                                        gAACACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                           >  1:1151218/1‑106 (MQ=255)                                                                                                                        gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                           <  2:1151218/106‑1 (MQ=255)                                                                                                                         aaaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCg                                                                                         >  2:479951/1‑59 (MQ=255)                                                                                                                         aaaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCg                                                                                         <  1:479951/59‑1 (MQ=255)                                                                                                                                gtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAATg  <  1:728151/139‑1 (MQ=255)                                                                                                                                   gtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACaa                                                  <  1:1554470/88‑1 (MQ=255)                                                                                                                                   gtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACaa                                                  >  2:1554470/1‑88 (MQ=255)                                                                                                                                       |                                                                                                                                    TTGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAATG  >  NC_000913/892735‑893000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑