Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	911,132	A→G	intergenic (‑83/+50)	poxB ← / ← hcr	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	911,132	0	A	G	100.0%	181.4 / NA	50	intergenic (‑83/+50)	poxB/hcr	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/22);  total (28/22)

AACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCG  >  NC_000913/911035‑911261                                                                                                  |                                                                                                                                  aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt                                                                                                                                 >  2:1568010/1‑100 (MQ=255) aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt                                                                                                                                 <  1:1568010/100‑1 (MQ=255)     gtttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGTAATATCATCGGGCATAAg                                                                                      <  2:939915/139‑1 (MQ=255)                            ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACg                                                                                                                             <  1:861747/77‑1 (MQ=255)                            ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACg                                                                                                                             >  2:861747/1‑77 (MQ=255)                            ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGaa                                                                                                      <  1:706893/100‑1 (MQ=255)                            ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGaa                                                                                                      >  2:706893/1‑100 (MQ=255)                            ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATcc                                                               >  2:1072992/1‑139 (MQ=255)                                ccgtcagatgaagTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAACGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCt                                                           >  2:733153/13‑139 (MQ=255)                                aaTGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt                                                                                                                                 <  1:1236653/69‑1 (MQ=255)                                 aTGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTg                                                          >  1:581437/1‑139 (MQ=255)                                     gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGa                                                      >  2:1439677/1‑139 (MQ=255)                                      aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCg                                                                           >  2:1392/1‑117 (MQ=255)                                      aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCg                                                                           <  1:1392/117‑1 (MQ=255)                                          cGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCa                                                 >  1:909164/1‑139 (MQ=255)                                                cAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCa                                           <  1:1439677/139‑1 (MQ=255)                                                    tttAGTTCATCTGAGGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc                                       <  1:1072992/139‑1 (MQ=255)                                                     ttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATa                                                                                        <  2:853320/89‑1 (MQ=255)                                                     ttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATa                                                                                        >  1:853320/1‑89 (MQ=255)                                                     ttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa                                      >  1:693469/1‑139 (MQ=255)                                                       aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgag                                                                         >  1:1291013/1‑102 (MQ=255)                                                       aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgag                                                                         <  2:1291013/102‑1 (MQ=255)                                                         ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAc                                  >  2:1688972/1‑139 (MQ=255)                                                             tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTaa                              >  2:737259/1‑139 (MQ=255)                                                              cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGc                                                                            >  1:525709/1‑92 (MQ=255)                                                              cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGc                                                                            <  2:525709/92‑1 (MQ=255)                                                              cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg                                             <  1:941857/123‑1 (MQ=255)                                                              cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg                                             >  2:941857/1‑123 (MQ=255)                                                                 aCGGAGGGGGAAGGGCTGGGAGAGAAAGGAGGCGCTaa                                                                                                                               >  2:1128588/1‑38 (MQ=255)                                                                 aCGGAGGGGGAAGGGATGTGAGAGAAAGGAGGCGCTaa                                                                                                                               <  1:1128588/38‑1 (MQ=255)                                                                      gggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCg                     >  1:775081/1‑139 (MQ=255)                                                                        gggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAt                   >  1:525286/1‑139 (MQ=255)                                                                          gAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAttt                 <  1:737259/139‑1 (MQ=255)                                                                           aaGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc                                       <  2:1481896/116‑1 (MQ=255)                                                                           aaGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc                                       >  1:1481896/1‑116 (MQ=255)                                                                           aaGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTc                >  2:1285714/1‑139 (MQ=255)                                                                              ggATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGc             <  2:693469/139‑1 (MQ=255)                                                                                  gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGc                                                                            <  1:772334/72‑1 (MQ=255)                                                                                  gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGc                                                                            >  2:772334/1‑72 (MQ=255)                                                                                  gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg                                                       >  2:1668139/1‑93 (MQ=255)                                                                                  gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg                                                       <  1:1668139/93‑1 (MQ=255)                                                                                  gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg                                             >  1:912441/1‑103 (MQ=255)                                                                                  gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg                                             <  2:912441/103‑1 (MQ=255)                                                                                    gagagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATa                                                                                        <  2:121666/58‑1 (MQ=255)                                                                                    gagagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATa                                                                                        >  1:121666/1‑58 (MQ=255)                                                                                     agagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                      <  1:128570/59‑1 (MQ=255)                                                                                     agagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                      >  2:128570/1‑59 (MQ=255)                                                                                         aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGAt                                                     <  1:1338168/88‑1 (MQ=255)                                                                                         aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGAt                                                     >  2:1338168/1‑88 (MQ=255)                                                                                         aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCg  >  1:1220576/1‑139 (MQ=255)                                                                                                  |                                                                                                                                  AACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCG  >  NC_000913/911035‑911261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑