Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,349	G→A	L156L (CTG→TTG)	hcp ←	hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,349	0	G	A	100.0%	207.3 / NA	65	L156L (CTG→TTG)	hcp	hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

CGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCG  >  NC_000913/913220‑913482                                                                                                                                  |                                                                                                                                        cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCAcagcag                                                                                                                                >  1:352009/1‑139 (MQ=255)              agacaaGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCgaga                                                                                                                   <  1:1124133/134‑1 (MQ=255)                  cAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGt                                                                                                               <  1:1268797/139‑1 (MQ=255)                              cACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                   <  2:352009/139‑1 (MQ=255)                               aCGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgc                                                                                                  <  2:523075/139‑1 (MQ=255)                                cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgcc                                                                                                 >  1:669552/1‑139 (MQ=255)                                cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAAAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTTAGGGCATACAAGCACAGCAGACCCAGGCCGAGAAAGTTTTCGccctgtctc                                                                                                 >  2:1124133/1‑132 (MQ=255)                                                  cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGcc                                                                                                                       >  2:1311875/1‑99 (MQ=255)                                                  cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGcc                                                                                                                       <  1:1311875/99‑1 (MQ=255)                                                  cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              <  1:914229/124‑1 (MQ=255)                                                  cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              >  2:914229/1‑124 (MQ=255)                                                  cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt                                                                                    <  2:1567194/134‑1 (MQ=255)                                                  cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt                                                                                    >  1:1567194/1‑134 (MQ=255)                                                  cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑ggg                                                                             >  1:704329/1‑139 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACg                                                                                                                             <  2:864216/90‑1 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACg                                                                                                                             >  1:864216/1‑90 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCgag                                                                                                                    >  2:792006/1‑99 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCgag                                                                                                                    <  1:792006/99‑1 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              <  2:205258/121‑1 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              >  1:205258/1‑121 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTa                                                                          >  1:633597/1‑139 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTa                                                                          >  1:120417/1‑139 (MQ=255)                                                     ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTa                                                                          >  2:966258/1‑139 (MQ=255)                                                          aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTc                                                                     >  1:427968/1‑139 (MQ=255)                                                            atatCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCttt                                                                                            <  2:682979/116‑1 (MQ=255)                                                            atatCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCttt                                                                                            >  1:682979/1‑116 (MQ=255)                                                               tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTc                                                                                                           >  1:1526892/1‑98 (MQ=255)                                                               tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTc                                                                                                           <  2:1526892/98‑1 (MQ=255)                                                                cGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCtgctgc                                                               <  2:633597/139‑1 (MQ=255)                                                                 gTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                   <  2:646721/104‑1 (MQ=255)                                                                 gTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                   >  1:646721/1‑104 (MQ=255)                                                                 gTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              >  2:8419/1‑109 (MQ=255)                                                                 gTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              <  1:8419/109‑1 (MQ=255)                                                                    gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCgag                                                                                                                    >  2:359708/1‑84 (MQ=255)                                                                    gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCgag                                                                                                                    <  1:359708/84‑1 (MQ=255)                                                                     tCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCtt                                                                                             >  1:1531463/1‑106 (MQ=255)                                                                     tCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCtt                                                                                             <  2:1531463/106‑1 (MQ=255)                                                                       gTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                                    <  2:797647/97‑1 (MQ=255)                                                                       gTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                                    >  1:797647/1‑97 (MQ=255)                                                                          ttcaCCGAGAAGATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                                  >  2:981284/4‑112 (MQ=255)                                                                          ttGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                                  <  1:981284/112‑1 (MQ=255)                                                                            gACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAg                                                   <  2:669552/139‑1 (MQ=255)                                                                            gACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAg                                                   <  1:966258/139‑1 (MQ=255)                                                                             aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGc                                                  >  2:840313/1‑139 (MQ=255)                                                                               cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGGGTAAATTCTGCTGCCTGACGTTGCAGc                                                  >  1:1484674/1‑139 (MQ=255)                                                                                       tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCTAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAg                                                   <  2:586207/128‑1 (MQ=255)                                                                                       tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAg                                                   >  1:586207/1‑128 (MQ=255)                                                                                           cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGc                                                                                                                        >  2:937941/1‑57 (MQ=255)                                                                                           cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGc                                                                                                                        <  1:937941/57‑1 (MQ=255)                                                                                                tCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgc                                                                                                  <  1:269660/74‑1 (MQ=255)                                                                                                tCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgc                                                                                                  >  2:269660/1‑74 (MQ=255)                                                                                                tCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCa                               >  1:665714/1‑139 (MQ=255)                                                                                                         gccgccGCACCTTTTAGGCCATACAAGCACTGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTGTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGTGATCGTCGCTCCCCAGTTgta                      >  2:787103/1‑137 (MQ=255)                                                                                                            gccgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                   <  2:427968/139‑1 (MQ=255)                                                                                                              cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              >  2:854691/1‑64 (MQ=255)                                                                                                              cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                              <  1:854691/64‑1 (MQ=255)                                                                                                              cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCGGTTGCAGGTCa                 >  1:1102021/1‑139 (MQ=255)                                                                                                              cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCa                 >  2:1371596/1‑139 (MQ=255)                                                                                                                 aCCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                                   >  1:287272/1‑72 (MQ=255)                                                                                                                 aCCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                                   <  2:287272/72‑1 (MQ=255)                                                                                                                   cTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt            <  2:1102021/139‑1 (MQ=255)                                                                                                                     ttCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGc                                  >  1:214677/1‑115 (MQ=255)                                                                                                                     ttCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGc                                  <  2:214677/115‑1 (MQ=255)                                                                                                                           ccATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTAt    <  2:665714/139‑1 (MQ=255)                                                                                                                             aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCg  >  1:1682166/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        CGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTA‑‑GGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCG  >  NC_000913/913220‑913482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑