Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,109	G→A	*270* (TAG→TAA)	pabC →	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,109	0	G	A	100.0%	156.9 / NA	49	*270* (TAG→TAA)	pabC	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/23);  total (26/23)

TCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTA  >  NC_000913/1153975‑1154238                                                                                                                                       |                                                                                                                                  tCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCAt                                                                                                                               >  2:751111/1‑139 (MQ=255) tCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCAt                                                                                                                               >  2:1515365/1‑139 (MQ=255)           cagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtg                                                                                                                     <  1:751111/139‑1 (MQ=255)            agatgagatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                    <  1:1515365/139‑1 (MQ=255)                  gatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGa                                                                                                              <  2:1391323/139‑1 (MQ=255)                             gTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGATTTAATCATGAAAAAAgtg                                                                                                                     <  2:941371/121‑1 (MQ=255)                             gTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtg                                                                                                                     >  1:941371/1‑121 (MQ=255)                                aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                >  2:848433/1‑107 (MQ=255)                                aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                <  1:848433/107‑1 (MQ=255)                                        aTGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTggtggt                                                                                        >  2:571012/1‑139 (MQ=255)                                           ccAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACt                                                                                     <  1:571012/139‑1 (MQ=255)                                                    cccGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                            <  1:117508/123‑1 (MQ=255)                                                    cccGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                            >  2:117508/1‑123 (MQ=255)                                                         aTGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                >  1:403325/1‑82 (MQ=255)                                                         aTGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                <  2:403325/82‑1 (MQ=255)                                                                  tgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                            <  1:614057/109‑1 (MQ=255)                                                                  tgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                            >  2:614057/1‑109 (MQ=255)                                                                  tgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCg                                                              >  1:40317/1‑139 (MQ=255)                                                                  tgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCg                                                              >  1:212915/1‑139 (MQ=255)                                                                       aTGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGtgtg                                                                  >  1:854816/1‑130 (MQ=255)                                                                       aTGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGtgtg                                                                  <  2:854816/130‑1 (MQ=255)                                                                        tGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGa                                                        <  2:40317/139‑1 (MQ=255)                                                                         gTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTgg                                                         <  2:944751/137‑1 (MQ=255)                                                                         gTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTAGCGCCGCTGGTGTGGGCGTCTgg                                                         >  1:944751/1‑137 (MQ=255)                                                                                 ttCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTgg                                                         >  1:1252155/1‑129 (MQ=255)                                                                                 ttCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTgg                                                         <  2:1252155/129‑1 (MQ=255)                                                                                    gTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATc                                            >  2:874725/1‑139 (MQ=255)                                                                                                  atGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCaaa                              <  1:874725/139‑1 (MQ=255)                                                                                                   tGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                <  2:1522912/40‑1 (MQ=255)                                                                                                   tGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                >  1:1522912/1‑40 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                    <  2:1331493/48‑1 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                    >  1:1331493/1‑48 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGtgtg                                                                  <  2:781417/98‑1 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGtgtg                                                                  >  1:781417/1‑98 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGa                                                        <  2:1385466/108‑1 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGa                                                        >  1:1385466/1‑108 (MQ=255)                                                                                                              ccccACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                            <  1:587824/65‑1 (MQ=255)                                                                                                              ccccACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                            >  2:587824/1‑65 (MQ=255)                                                                                                                ccACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATaa                                                                                                           <  1:809399/48‑1 (MQ=255)                                                                                                                ccACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATaa                                                                                                           >  2:809399/1‑48 (MQ=255)                                                                                                                   cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCa                                <  1:34504/120‑1 (MQ=255)                                                                                                                   cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCa                                >  2:34504/1‑120 (MQ=255)                                                                                                                   cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAagaaga             >  1:270818/1‑139 (MQ=255)                                                                                                                        tgAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGaa                                                       >  2:1192568/1‑92 (MQ=255)                                                                                                                        tgAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGaa                                                       <  1:1192568/92‑1 (MQ=255)                                                                                                                            cgcCCGAATTAATCATGAAATAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGcc                                               <  1:1019412/96‑1 (MQ=255)                                                                                                                            cgcCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGcc                                               >  2:1019412/1‑96 (MQ=255)                                                                                                                             gcCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATAttt   >  2:376649/1‑139 (MQ=255)                                                                                                                              cccGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTa  >  2:1364838/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                  TCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTA  >  NC_000913/1153975‑1154238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑