Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 115,661 T→C M22V (ATG→GTG)  hofC ← assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913115,6610TC98.3% 207.0 / NA 59M22V (ATG→GTG) hofCassembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (30/28);  minor base A (1/0);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATGAATGACTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGTTT  >  NC_000913/115530‑115795
                                                                                                                                   |                                                                                                                                      
atgaatgaCTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCt                                                                                                                                 <  2:783929/139‑1 (MQ=255)
atgaatgaCTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCt                                                                                                                                 <  1:516728/139‑1 (MQ=255)
      gaCTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCa                                                                                                                           >  2:1629507/1‑139 (MQ=255)
                          ctcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACTATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcc                                                                                                       >  2:188862/1‑139 (MQ=255)
                           tcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGc                                                                                                                       <  2:1504058/122‑1 (MQ=255)
                           tcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGc                                                                                                                       >  1:1504058/1‑122 (MQ=255)
                           tcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcca                                                                                                      >  1:954817/1‑139 (MQ=255)
                               cACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgcc                                                                                                  <  2:1236803/139‑1 (MQ=255)
                                  tGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgc                                                                                               >  1:1169206/1‑139 (MQ=255)
                                    cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgcgc                                                                                             >  1:1665971/1‑139 (MQ=255)
                                              aTGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAg                                                                                                <  1:1410698/126‑1 (MQ=255)
                                              aTGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAg                                                                                                >  2:1410698/1‑126 (MQ=255)
                                                     ttCGCTTCAGGCTTAGTGTGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCg                                                                                                                     <  2:1470468/98‑1 (MQ=255)
                                                     ttCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCg                                                                                                                     >  1:1470468/1‑98 (MQ=255)
                                                     ttCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGc                                                                            <  1:892601/139‑1 (MQ=255)
                                                             aGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                                    <  2:1169206/139‑1 (MQ=255)
                                                               gCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCa                                                                                                                           <  2:566243/82‑1 (MQ=255)
                                                               gCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCa                                                                                                                           >  1:566243/1‑82 (MQ=255)
                                                                   aGTGGGGTAACTATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATcccc                                                              <  1:188862/139‑1 (MQ=255)
                                                                   aGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATcccc                                                              >  1:104497/1‑139 (MQ=255)
                                                                          tAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                             >  1:1583093/1‑85 (MQ=255)
                                                                          tAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                             <  2:1583093/85‑1 (MQ=255)
                                                                            aCCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                                    <  1:1573048/124‑1 (MQ=255)
                                                                            aCCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                                    >  2:1573048/1‑124 (MQ=255)
                                                                                    ttGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCa                                                                                           <  1:295481/93‑1 (MQ=255)
                                                                                    ttGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCa                                                                                           >  2:295481/1‑93 (MQ=255)
                                                                                    ttGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAActc                                             >  2:417615/1‑139 (MQ=255)
                                                                                           aGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCg                                                                      <  1:728189/107‑1 (MQ=255)
                                                                                           aGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCg                                                                      >  2:728189/1‑107 (MQ=255)
                                                                                           aGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                                      <  1:1428436/139‑1 (MQ=255)
                                                                                              gCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                                   <  2:1077144/75‑1 (MQ=255)
                                                                                              gCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                                   >  1:1077144/1‑75 (MQ=255)
                                                                                              gCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTAGCTTCCTCGCCATGCGGCATCCCCAGTACGCGGATTAACTTTTCAAAggtg                                   >  2:511460/1‑139 (MQ=255)
                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCgg                                                                                                              >  2:187716/1‑58 (MQ=255)
                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCgg                                                                                                              <  1:187716/58‑1 (MQ=255)
                                                                                                       aGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTc                                                                                                                   <  2:294745/50‑1 (MQ=255)
                                                                                                       aGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTc                                                                                                                   >  1:294745/1‑50 (MQ=255)
                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                                  >  2:243178/1‑49 (MQ=255)
                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                                  <  1:243178/49‑1 (MQ=255)
                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCtt                                           >  1:844859/1‑120 (MQ=255)
                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCtt                                           <  2:844859/120‑1 (MQ=255)
                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTg                        >  2:8842/1‑139 (MQ=255)
                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGGTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGAt                                                   <  2:160189/112‑1 (MQ=255)
                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGATATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGAt                                                   >  1:160189/1‑112 (MQ=255)
                                                                                                             gTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGctct                    <  1:417615/139‑1 (MQ=255)
                                                                                                              tACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTg                                                                                                                        >  1:14491/1‑38 (MQ=255)
                                                                                                              tACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTg                                                                                                                        <  2:14491/38‑1 (MQ=255)
                                                                                                                cGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                                >  2:1449716/1‑124 (MQ=255)
                                                                                                                cGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                                <  1:1449716/124‑1 (MQ=255)
                                                                                                                cGGCTCTCTGCCCATAGCAACCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACg                 >  1:981529/1‑139 (MQ=255)
                                                                                                                   ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                             <  1:626973/44‑1 (MQ=255)
                                                                                                                   ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                             >  2:626973/1‑44 (MQ=255)
                                                                                                                        tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                          <  2:1469974/90‑1 (MQ=255)
                                                                                                                        tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                          >  1:1469974/1‑90 (MQ=255)
                                                                                                                        tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCa         >  2:1552040/1‑139 (MQ=255)
                                                                                                                          cccATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGTATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                                <  2:465776/114‑1 (MQ=255)
                                                                                                                          cccATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGTATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                                >  1:465776/1‑114 (MQ=255)
                                                                                                                              tAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGtt   <  2:1665971/139‑1 (MQ=255)
                                                                                                                               aGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGttt  >  1:1468428/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                      
ATGAATGACTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGTTT  >  NC_000913/115530‑115795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑