Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	117,798	A→G	L283P (CTA→CCA)	nadC ←	quinolinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	117,798	0	A	G	100.0%	192.5 / NA	53	L283P (CTA→CCA)	nadC	quinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

AAAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTT  >  NC_000913/117664‑117930                                                                                                                                       |                                                                                                                                     aaaGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATGAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGgcgc                                                                                                                                  >  1:884304/1‑139 (MQ=255)         gAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                                 <  1:611180/132‑1 (MQ=255)         gAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                                 >  2:611180/1‑132 (MQ=255)              ttGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATa                                                                                                                     >  2:227064/1‑139 (MQ=255)                                            ggggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       <  1:1453893/139‑1 (MQ=255)                                              ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAg                                                                                     >  1:623671/1‑139 (MQ=255)                                                   ttATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGttt                                                                                <  1:732801/139‑1 (MQ=255)                                                       ccgcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCaaa                                                                                              >  1:1543082/1‑121 (MQ=255)                                                       ccgcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCaaa                                                                                              <  2:1543082/121‑1 (MQ=255)                                                       ccgcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       <  2:756112/128‑1 (MQ=255)                                                       ccgcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       >  1:756112/1‑128 (MQ=255)                                                          cccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGtttt                                                                               <  2:904507/133‑1 (MQ=255)                                                          cccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGtttt                                                                               >  1:904507/1‑133 (MQ=255)                                                           ccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       <  1:23331/124‑1 (MQ=255)                                                           ccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       >  2:23331/1‑124 (MQ=255)                                                            cgcgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGa                                                                       <  2:623671/139‑1 (MQ=255)                                                              cgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       >  2:806437/1‑121 (MQ=255)                                                              cgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       <  1:806437/121‑1 (MQ=255)                                                                    aGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCATGCAGTGTTTTGTCAGTGACGTTGCCa                                                               >  1:782613/1‑139 (MQ=255)                                                                      cGGATGAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGa                                                             <  2:884304/139‑1 (MQ=255)                                                                      cGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCATGCAGTGTTTTGTCAGTGACGTTGCCAGa                                                             <  2:782613/139‑1 (MQ=255)                                                                             aTCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       >  2:827709/1‑106 (MQ=255)                                                                             aTCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       <  1:827709/106‑1 (MQ=255)                                                                                  tCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAg                                                 >  1:315664/1‑139 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGa                                                                       >  2:851845/1‑107 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGa                                                                       <  1:851845/107‑1 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACg                                                                     >  1:1214194/1‑109 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACg                                                                     <  2:1214194/109‑1 (MQ=255)                                                                                                aaaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTgg                                   <  1:983694/139‑1 (MQ=255)                                                                                                 aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAg                                                 <  1:253305/124‑1 (MQ=255)                                                                                                 aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAg                                                 >  2:253305/1‑124 (MQ=255)                                                                                                   cGCATTGAAAGGTTGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGc                                >  1:119539/1‑139 (MQ=255)                                                                                                   cGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGt                                      >  1:1500845/1‑133 (MQ=255)                                                                                                   cGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGt                                      <  2:1500845/133‑1 (MQ=255)                                                                                                   cGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGc                                >  1:1668425/1‑139 (MQ=255)                                                                                                      aTTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGtt                             >  1:540473/1‑139 (MQ=255)                                                                                                              gTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCt                     >  2:735284/1‑139 (MQ=255)                                                                                                               tCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTc                                                                                                                <  2:826298/47‑1 (MQ=255)                                                                                                               tCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTc                                                                                                                >  1:826298/1‑47 (MQ=255)                                                                                                               tCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCAc                                                                                         >  1:625789/1‑70 (MQ=255)                                                                                                               tCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCAc                                                                                         <  2:625789/70‑1 (MQ=255)                                                                                                                 gAGTGTTTGTACGTGTTTAGTTGGCGAACCGACGGAGATAAAGTCCACGCCCTTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg                  <  2:1668425/139‑1 (MQ=255)                                                                                                                 gAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg                  <  1:227064/139‑1 (MQ=255)                                                                                                                  aGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGGCACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcgc                 >  1:95019/1‑139 (MQ=255)                                                                                                                      cTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAg                                                    <  1:218490/100‑1 (MQ=255)                                                                                                                      cTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAg                                                    >  2:218490/1‑100 (MQ=255)                                                                                                                      cTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt             <  2:119539/139‑1 (MQ=255)                                                                                                                         gTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCATGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAtctg          >  2:78030/1‑139 (MQ=255)                                                                                                                         gTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCa                                                               <  2:677645/86‑1 (MQ=255)                                                                                                                         gTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCa                                                               >  1:677645/1‑86 (MQ=255)                                                                                                                                tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAg                                                              >  2:16366/1‑80 (MQ=255)                                                                                                                                tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAg                                                              <  1:16366/80‑1 (MQ=255)                                                                                                                                tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATctgttctgtt   >  2:503460/1‑139 (MQ=255)                                                                                                                                 ttagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                        <  1:702724/53‑1 (MQ=255)                                                                                                                                 ttagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                        >  2:702724/1‑53 (MQ=255)                                                                                                                                 ttagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGttt  <  1:735284/139‑1 (MQ=255)                                                                                                                                  tagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAg                                                 >  1:163789/1‑91 (MQ=255)                                                                                                                                  tagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAg                                                 <  2:163789/91‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     AAAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTT  >  NC_000913/117664‑117930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑