Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 125,227 T→C R737R (CGT→CGC)  aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913125,2270TC100.0% 186.9 / NA 52R737R (CGT→CGC) aceEpyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

TGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCC  >  NC_000913/125094‑125355
                                                                                                                                     |                                                                                                                                
tGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGt                                                                                                                             >  2:1372213/1‑139 (MQ=255)
  aaCGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTcc                                                                                                                           >  1:1497588/1‑139 (MQ=255)
      aaaaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGa                                                                                                                       <  1:1313211/139‑1 (MQ=255)
       aaaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGaa                                                                                                                      >  2:265766/1‑139 (MQ=255)
        aaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     <  2:1497588/139‑1 (MQ=255)
        aaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     <  2:1341099/139‑1 (MQ=255)
                                 aaGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTa                                                                                            >  1:1516762/1‑139 (MQ=255)
                                      gCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCg                                                                                       <  1:1372213/139‑1 (MQ=255)
                                       cTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGt                                                                                      >  2:296467/1‑139 (MQ=255)
                                       cTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGt                                                                                      >  2:1185659/1‑139 (MQ=255)
                                               ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCa                                                                                                                                >  2:394905/1‑89 (MQ=255)
                                                         aaGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAg                                                                    <  2:43198/139‑1 (MQ=255)
                                                             tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg                                                                <  1:823335/139‑1 (MQ=255)
                                                               tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     >  2:516218/1‑84 (MQ=255)
                                                               tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     <  1:516218/84‑1 (MQ=255)
                                                                cTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAcc                                                             >  1:921004/1‑139 (MQ=255)
                                                                 tACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCg                                                                                                                          >  1:1545606/1‑77 (MQ=255)
                                                                 tACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCg                                                                                                                          <  2:1545606/77‑1 (MQ=255)
                                                                     aaCTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCtt                                                        >  1:1386132/1‑139 (MQ=255)
                                                                      aCTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     >  2:1669133/1‑77 (MQ=255)
                                                                      aCTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     <  1:1669133/77‑1 (MQ=255)
                                                                       cTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCa                                                      <  1:1640406/139‑1 (MQ=255)
                                                                           aaaCTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGa                                                  >  2:1026392/1‑139 (MQ=255)
                                                                           aaaCTATTGAAGGTAGCAAAGGTAAAGTTC‑GCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     <  2:645337/71‑1 (MQ=255)
                                                                           aaaCTATTGAAGGTAGCAAAGGTAAAGTTC‑GCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     >  1:645337/1‑71 (MQ=255)
                                                                                  tGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGgcg                                           >  2:1681567/1‑139 (MQ=255)
                                                                                      ggTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg                                       <  1:1026392/139‑1 (MQ=255)
                                                                                      ggTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg                                       >  1:1269458/1‑139 (MQ=255)
                                                                                      ggTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg                                       <  1:1681567/139‑1 (MQ=255)
                                                                                         aGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     >  1:1621709/1‑58 (MQ=255)
                                                                                         aGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     <  2:1621709/58‑1 (MQ=255)
                                                                                         aGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATg                                    >  1:37566/1‑139 (MQ=255)
                                                                                          gCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATgg                                   <  2:1269458/139‑1 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCAc                                                                                                                               <  2:536235/46‑1 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCAc                                                                                                                               >  1:536235/1‑46 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     <  2:929783/56‑1 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                     >  1:929783/1‑56 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAg                                                 <  2:152958/124‑1 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAg                                                 >  1:152958/1‑124 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt                                  >  2:1231156/1‑139 (MQ=255)
                                                                                           cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt                                  <  2:921004/139‑1 (MQ=255)
                                                                                             aaGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCa                                <  1:1231156/139‑1 (MQ=255)
                                                                                                              ctCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGa                                                                                                                       <  2:1000226/35‑1 (MQ=255)
                                                                                                              ctCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGa                                                                                                                       >  1:1000226/1‑35 (MQ=255)
                                                                                                               tCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGTGATCCTGGCGAAAGATTACGGCGTAGGTTCt                                                                               >  1:1104231/1‑74 (MQ=255)
                                                                                                               tCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCt                                                                               <  2:1104231/74‑1 (MQ=255)
                                                                                                                        gTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGa                                                               >  1:236014/1‑81 (MQ=255)
                                                                                                                        gTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGa                                                               <  2:236014/81‑1 (MQ=255)
                                                                                                                        gTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCa     >  1:130987/1‑139 (MQ=255)
                                                                                                                         ttCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCAc    <  2:1516762/139‑1 (MQ=255)
                                                                                                                         ttCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCAc    <  2:1386132/139‑1 (MQ=255)
                                                                                                                           cTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCAccc  >  2:232899/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                
TGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCC  >  NC_000913/125094‑125355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑