Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	143,897	C→T	L66L (CTG→TTG)	yadH →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	143,897	0	C	T	100.0%	168.7 / NA	53	L66L (CTG→TTG)	yadH	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

TCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGC  >  NC_000913/143769‑144021                                                                                                                                 |                                                                                                                             tCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCTGCTATATGCAGTTCATCGTACCGGGGTTGATCatgat                                                                                                                    >  2:1079052/1‑139 (MQ=255)  cTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCatgatg                                                                                                                   <  2:1227035/139‑1 (MQ=255)            aCGCTGGTGCCGCCAGTCATCAGCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGa                                                                                                            <  2:1260693/136‑1 (MQ=255)            aCGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGa                                                                                                            >  1:1260693/1‑136 (MQ=255)            aCGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCa                                                                                                         >  2:699849/1‑139 (MQ=255)               cTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCa                                                                                                      >  1:1571631/1‑139 (MQ=255)                  gTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATg                                                                                                   <  2:1571631/139‑1 (MQ=255)                    gccgccAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGcc                                                                                                 <  1:1079052/139‑1 (MQ=255)                          aGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGcc                                                                                           <  1:254605/139‑1 (MQ=255)                                     tGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCatgatg                                                                                                                   >  2:1589240/1‑104 (MQ=255)                                     tGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCatgatg                                                                                                                   <  1:1589240/104‑1 (MQ=255)                                             tactttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCAtttt                                                                        >  2:119603/1‑139 (MQ=255)                                               ctttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGcc                                                                                           <  1:1578058/118‑1 (MQ=255)                                               ctttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGcc                                                                                           >  2:1578058/1‑118 (MQ=255)                                                    ttatCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCa                                                                                                      >  1:1354087/1‑102 (MQ=255)                                                    ttatCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCa                                                                                                      <  2:1354087/102‑1 (MQ=255)                                                     tatCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGcc                                                                                           <  1:1610743/112‑1 (MQ=255)                                                     tatCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGcc                                                                                           >  2:1610743/1‑112 (MQ=255)                                                     tatCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGcc                                                                <  1:243576/139‑1 (MQ=255)                                                                aCCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCg                                                     <  1:699849/139‑1 (MQ=255)                                                                aCCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCg                                                     <  1:806965/139‑1 (MQ=255)                                                                 ccTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGt                                                    >  2:575178/1‑139 (MQ=255)                                                                     aTTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGt                                                                                                                  <  1:399078/73‑1 (MQ=255)                                                                     aTTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGt                                                                                                                  >  2:399078/1‑73 (MQ=255)                                                                     aTTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGt                                                            <  2:1120870/127‑1 (MQ=255)                                                                     aTTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGt                                                            >  1:1120870/1‑127 (MQ=255)                                                                        ggTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTg                                             <  1:119603/139‑1 (MQ=255)                                                                          ttCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGaa                                           >  1:1196296/1‑139 (MQ=255)                                                                            cgcgTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCAttttt                                                                       >  2:1467498/1‑109 (MQ=255)                                                                            cgcgTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCAttttt                                                                       <  1:1467498/109‑1 (MQ=255)                                                                             gcgTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGcc                                                                                                 >  1:521269/1‑82 (MQ=255)                                                                             gcgTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGcc                                                                                                 <  2:521269/82‑1 (MQ=255)                                                                                       gATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTc                                                          <  1:225208/111‑1 (MQ=255)                                                                                       gATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTc                                                          >  2:225208/1‑111 (MQ=255)                                                                                         tatGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGtt                                                           <  2:1470848/108‑1 (MQ=255)                                                                                         tatGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGtt                                                           >  1:1470848/1‑108 (MQ=255)                                                                                               tGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAgcgc                           >  1:475965/1‑134 (MQ=255)                                                                                               tGGCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAgcgc                           <  2:475965/134‑1 (MQ=255)                                                                                                 gCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAaga                                         <  2:951866/118‑1 (MQ=255)                                                                                                 gCTTCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAaga                                         >  1:951866/1‑118 (MQ=255)                                                                                                   ttCAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCATGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGa                  <  2:1196296/139‑1 (MQ=255)                                                                                                     cAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACt                <  2:293208/139‑1 (MQ=255)                                                                                                     cAGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACt                >  1:293208/1‑139 (MQ=255)                                                                                                      aGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGcc                                                                                                 <  2:1169979/57‑1 (MQ=255)                                                                                                      aGCTATATGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGcc                                                                                                 >  1:1169979/1‑57 (MQ=255)                                                                                                            atGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCa         >  1:878533/1‑139 (MQ=255)                                                                                                             tGCAGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCat        >  2:1436302/1‑139 (MQ=255)                                                                                                                aGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGt                                                                                 >  1:92761/1‑63 (MQ=255)                                                                                                                aGTTCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGt                                                                                 <  2:92761/63‑1 (MQ=255)                                                                                                                  ttCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTg   >  1:1123626/1‑139 (MQ=255)                                                                                                                   tCATCGTACCGGGGTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGc  >  1:164668/1‑139 (MQ=255)                                                                                                                              gggTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACt                >  2:152424/1‑114 (MQ=255)                                                                                                                              gggTTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACt                <  1:152424/114‑1 (MQ=255)                                                                                                                                 |                                                                                                                             TCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGC  >  NC_000913/143769‑144021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑