Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,491,878	G→A	intergenic (+28/+44)	ydcA → / ← hokB	putative periplasmic protein/toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,491,878	0	G	A	100.0%	208.9 / NA	64	intergenic (+28/+44)	ydcA/hokB	putative periplasmic protein/toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/30);  total (34/30)

GTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAG  >  NC_000913/1491756‑1492005                                                                                                                           |                                                                                                                                gTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTg                                                                                                                           <  1:1194784/129‑1 (MQ=255) gTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTATCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTg                                                                                                                           >  2:1194784/1‑129 (MQ=255)      aaaGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGcgcg                                                                                                            >  2:837663/1‑139 (MQ=255)                    acacTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCcgc                                                                                              >  1:983418/1‑139 (MQ=255)                          tACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCAGGGAAAATCCCCGCGCTTGc                                                                                        >  2:168055/1‑139 (MQ=255)                           aCGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGcc                                                                                       <  1:601892/139‑1 (MQ=255)                                    ggcAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                              >  1:1441402/1‑139 (MQ=255)                                    ggcAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                              >  2:1295421/1‑139 (MQ=255)                                    ggcAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCAGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                              <  1:168055/139‑1 (MQ=255)                                                            tCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagca                                                       >  1:1295914/1‑138 (MQ=255)                                                            tCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagca                                                       <  2:1295914/138‑1 (MQ=255)                                                             cTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAc                                                     <  1:1295421/139‑1 (MQ=255)                                                             cTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAc                                                     <  1:837663/139‑1 (MQ=255)                                                             cTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAc                                                     <  2:1559820/139‑1 (MQ=255)                                                                    tGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTa                                              >  2:775411/1‑139 (MQ=255)                                                                     gCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTAt                                             >  2:1105858/1‑139 (MQ=255)                                                                      cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                      >  2:728532/1‑97 (MQ=255)                                                                      cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                      <  1:728532/97‑1 (MQ=255)                                                                      cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATc                                            >  1:38310/1‑139 (MQ=255)                                                                      cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATc                                            >  1:530717/1‑139 (MQ=255)                                                                      cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATc                                            >  1:1451467/1‑139 (MQ=255)                                                                        tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAgcg                                                            <  2:749304/121‑1 (MQ=255)                                                                        tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAgcg                                                            >  1:749304/1‑121 (MQ=255)                                                                        tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAc                                                     >  2:1284706/1‑128 (MQ=255)                                                                        tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAc                                                     <  1:1284706/128‑1 (MQ=255)                                                                        tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCAc                                          >  1:543278/1‑139 (MQ=255)                                                                              aaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAg                                                              >  1:387656/1‑113 (MQ=255)                                                                              aaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAg                                                              <  2:387656/113‑1 (MQ=255)                                                                                aaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCgg                                  >  1:274565/1‑139 (MQ=255)                                                                                     gCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagca                                                       <  1:344047/113‑1 (MQ=255)                                                                                     gCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagca                                                       >  2:344047/1‑113 (MQ=255)                                                                                      cACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGc                            <  2:274565/139‑1 (MQ=255)                                                                                           aCTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCTTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTc                       >  2:385115/1‑139 (MQ=255)                                                                                                 gtgtAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTagag                 <  1:775411/139‑1 (MQ=255)                                                                                                 gtgtAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTagag                 >  2:10516/1‑139 (MQ=255)                                                                                                 gtgtAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTa                    <  1:625597/136‑1 (MQ=255)                                                                                                 gtgtAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTa                    >  2:625597/1‑136 (MQ=255)                                                                                                   gtAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                      >  2:447812/1‑68 (MQ=255)                                                                                                   gtAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                      <  1:447812/68‑1 (MQ=255)                                                                                                     aaaaGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagc                                                        >  2:109943/1‑96 (MQ=255)                                                                                                     aaaaGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagc                                                        <  1:109943/96‑1 (MQ=255)                                                                                                     aaaaGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGtt             >  1:1081090/1‑139 (MQ=255)                                                                                                      aaaGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGttt            <  2:530717/139‑1 (MQ=255)                                                                                                      aaaGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGttt            >  2:261531/1‑139 (MQ=255)                                                                                                        aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGt          <  2:1441402/139‑1 (MQ=255)                                                                                                        aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGt          <  1:1105858/139‑1 (MQ=255)                                                                                                        aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGt          <  2:38310/139‑1 (MQ=255)                                                                                                        aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTCGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGGAGAGCGGTTGt          <  2:983418/139‑1 (MQ=255)                                                                                                         ggggTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGc                            <  2:313324/120‑1 (MQ=255)                                                                                                         ggggTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGc                            >  1:313324/1‑120 (MQ=255)                                                                                                            gTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                      >  2:1003600/1‑59 (MQ=255)                                                                                                            gTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                      <  1:1003600/59‑1 (MQ=255)                                                                                                            gTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTcc                                     >  2:213335/1‑108 (MQ=255)                                                                                                            gTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTcc                                     <  1:213335/108‑1 (MQ=255)                                                                                                             tGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGt     >  1:850790/1‑139 (MQ=255)                                                                                                             tGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGt     >  2:84261/1‑139 (MQ=255)                                                                                                              gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcc                                                    >  2:372712/1‑91 (MQ=255)                                                                                                              gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcc                                                    <  1:372712/91‑1 (MQ=255)                                                                                                              gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCAc                                          <  2:1476636/101‑1 (MQ=255)                                                                                                              gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCAc                                          >  1:1476636/1‑101 (MQ=255)                                                                                                              gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTACAGGCCATGAGCgcagca                                                       <  1:6701/88‑1 (MQ=255)                                                                                                              gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTACAGGCCATGAGCgcagca                                                       >  2:6701/1‑88 (MQ=255)                                                                                                                cATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAg  <  1:261531/139‑1 (MQ=255)                                                                                                                cATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAg  <  2:850790/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                GTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAG  >  NC_000913/1491756‑1492005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑