Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,560,898 C→T C11Y (TGC→TAC)  ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,8980CT100.0% 242.7 / NA 74C11Y (TGC→TAC) ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/38);  total (36/38)

TGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACAAAGCCTTTCACCTCTTTGTTCATCGCCAGTTGGTAGTTTTTCTGGAA  >  NC_000913/1560766‑1561032
                                                                                                                                    |                                                                                                                                      
tGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGtt                                                                                                                                  >  2:933574/1‑139 (MQ=255)
         ttttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTaaaa                                                                                                                         <  1:245602/139‑1 (MQ=255)
                   tAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCg                                                                                                                                    >  1:43434/1‑118 (MQ=255)
                   tAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCg                                                                                                                                    <  2:43434/118‑1 (MQ=255)
                     gCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAgg                                                                                                             <  1:933574/139‑1 (MQ=255)
                      cATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGt                                                                                                            >  1:764204/1‑139 (MQ=255)
                        tCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCg                                                                                                                              <  2:816217/119‑1 (MQ=255)
                        tCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCg                                                                                                                              >  1:816217/1‑119 (MQ=255)
                         cGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCAc                                                                                                         <  1:361548/139‑1 (MQ=255)
                           cTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGt                                                                                                       >  2:333825/1‑139 (MQ=255)
                               cgcgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGt                                                                                                            >  2:678939/1‑130 (MQ=255)
                               cgcgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGt                                                                                                            <  1:678939/130‑1 (MQ=255)
                               cgcgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                                                                                                   >  1:721503/1‑139 (MQ=255)
                                gcgGTCACCCGCCAGTAACCGCGCCGGATCAACAGGGATCAGGTGCGAGATAATAAACGTAATCACGCATACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAttt                                                                                                  >  2:154012/1‑139 (MQ=255)
                                  ggTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTAc                                                                                                <  1:333825/139‑1 (MQ=255)
                                   gTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTc                                                                                                           >  1:152463/1‑127 (MQ=255)
                                   gTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTc                                                                                                           <  2:152463/127‑1 (MQ=255)
                                    tCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTc                                                                                              <  2:764204/139‑1 (MQ=255)
                                      aCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt                                                                                            <  1:547023/139‑1 (MQ=255)
                                         cGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTa                                                                                                                            <  2:993665/104‑1 (MQ=255)
                                         cGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTa                                                                                                                            >  1:993665/1‑104 (MQ=255)
                                                aaCCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATa                                                                                                                       >  2:674902/1‑102 (MQ=255)
                                                aaCCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATa                                                                                                                       <  1:674902/102‑1 (MQ=255)
                                                  ccGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTa                                                                                                                            >  2:1459817/1‑95 (MQ=255)
                                                  ccGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTa                                                                                                                            <  1:1459817/95‑1 (MQ=255)
                                                     cgcCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACg                                                                                                        <  1:311272/112‑1 (MQ=255)
                                                     cgcCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACg                                                                                                        >  2:311272/1‑112 (MQ=255)
                                                      gcCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTa                                                                                                     >  2:1095275/1‑114 (MQ=255)
                                                      gcCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTa                                                                                                     <  1:1095275/114‑1 (MQ=255)
                                                                    gATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTcc                                                                                                                   <  1:1371662/86‑1 (MQ=255)
                                                                    gATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTcc                                                                                                                   >  2:1371662/1‑86 (MQ=255)
                                                                        aGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtat                                                                                       <  1:1116905/110‑1 (MQ=255)
                                                                        aGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtat                                                                                       >  2:1116905/1‑110 (MQ=255)
                                                                        aGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGAc                                                                          <  2:342808/123‑1 (MQ=255)
                                                                        aGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGAc                                                                          >  1:342808/1‑123 (MQ=255)
                                                                              gagaTAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAac                                                    >  2:449020/1‑139 (MQ=255)
                                                                               agaTAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCg                                                                                                                                    >  1:687205/1‑58 (MQ=255)
                                                                               agaTAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCg                                                                                                                                    <  2:687205/58‑1 (MQ=255)
                                                                                 ataataAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGGCCCCAGTAGCGTTGGCGTaaaa                                                                                                                         >  2:1198114/1‑67 (MQ=255)
                                                                                 ataataAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTaaaa                                                                                                                         <  1:1198114/67‑1 (MQ=255)
                                                                                 ataataAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAAcaca                                                 >  1:1395802/1‑139 (MQ=255)
                                                                                          gTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGtt                                                                                                      >  2:1500889/1‑77 (MQ=255)
                                                                                          gTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGtt                                                                                                      <  1:1500889/77‑1 (MQ=255)
                                                                                           tAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAAGACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGGCCTGTTCCAGCATGGGATTGAACACAAAGCCTTTCa                                       <  1:154012/139‑1 (MQ=255)
                                                                                            aaTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTc                                                                    <  2:935732/109‑1 (MQ=255)
                                                                                            aaTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTc                                                                    >  1:935732/1‑109 (MQ=255)
                                                                                            aaTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACAAAGCCttt                                         >  1:1614539/1‑136 (MQ=255)
                                                                                            aaTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACAAAGCCttt                                         <  2:1614539/136‑1 (MQ=255)
                                                                                             aTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTa                                                                                                     <  1:36105/75‑1 (MQ=255)
                                                                                             aTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTa                                                                                                     >  2:36105/1‑75 (MQ=255)
                                                                                             aTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGAc                                                                          <  1:233274/102‑1 (MQ=255)
                                                                                             aTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGAc                                                                          >  2:233274/1‑102 (MQ=255)
                                                                                                      aCGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATa                                                                                                                       <  2:336476/48‑1 (MQ=255)
                                                                                                      aCGCCCGCCACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATa                                                                                                                       >  1:336476/1‑48 (MQ=255)
                                                                                                          ccgccACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCGGAAGGTCACGTTATTTACTCAt                                                                                            >  1:1009827/1‑71 (MQ=255)
                                                                                                          ccgccACCACGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCGGAAGGTCACGTTATTTACTCAt                                                                                            <  2:1009827/71‑1 (MQ=255)
                                                                                                              caccacGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGa                                                                                                                >  1:92823/1‑47 (MQ=255)
                                                                                                              caccacGAGCAACAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGa                                                                                                                <  2:92823/47‑1 (MQ=38)
                                                                                                                ccacGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACaa                                                >  1:825050/1‑109 (MQ=255)
                                                                                                                ccacGAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACaa                                                <  2:825050/109‑1 (MQ=255)
                                                                                                                    gAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCa                                                                                                                  >  1:1554036/1‑39 (MQ=255)
                                                                                                                    gAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCa                                                                                                                  <  2:1554036/39‑1 (MQ=255)
                                                                                                                    gAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCa                                                                                             >  2:1581255/1‑60 (MQ=255)
                                                                                                                    gAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCa                                                                                             <  1:1581255/60‑1 (MQ=255)
                                                                                                                    gAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt                                                                                            <  1:1158193/61‑1 (MQ=255)
                                                                                                                    gAGCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt                                                                                            >  2:1158193/1‑61 (MQ=255)
                                                                                                                      gCACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACAAAGCCTTTCACCTCTTTGTTCATCGCCAGTTGGTAGt            <  1:449020/139‑1 (MQ=255)
                                                                                                                       cACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtat                                                                                       <  2:903981/63‑1 (MQ=255)
                                                                                                                       cACCAGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtat                                                                                       >  1:903981/1‑63 (MQ=255)
                                                                                                                           aGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt                                                                                         <  2:1670660/57‑1 (MQ=255)
                                                                                                                           aGCCCCCAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt                                                                                         >  1:1670660/1‑57 (MQ=255)
                                                                                                                              ccccAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCa                                                                                             >  2:568545/1‑50 (MQ=255)
                                                                                                                              ccccAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCa                                                                                             <  1:568545/50‑1 (MQ=255)
                                                                                                                                ccAGTAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACAAAGCCTTTCACCTCTTTGTTCATCGCCAGTTGGTAGTTTTTCTGGaa  <  2:1395802/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
TGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTGTTCCAGCATGGGATTGAACACAAAGCCTTTCACCTCTTTGTTCATCGCCAGTTGGTAGTTTTTCTGGAA  >  NC_000913/1560766‑1561032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: