Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,935	T→C	K89K (AAA→AAG)	yadV ←	putative periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,935	0	T	C	100.0%	256.2 / NA	71	K89K (AAA→AAG)	yadV	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/36);  total (35/36)

TTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGA  >  NC_000913/155808‑156064                                                                                                                                |                                                                                                                                  tttGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                          <  1:120920/137‑1 (MQ=255) tttGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                          >  2:120920/1‑137 (MQ=255)    gATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     >  1:1554708/1‑139 (MQ=255)                  tCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGgcggcg                                                                                                       <  2:1554708/139‑1 (MQ=255)                           ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaa                                                                                              >  1:1197224/1‑139 (MQ=255)                           ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaa                                                                                              >  2:1444767/1‑139 (MQ=255)                            gTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                             <  2:885298/139‑1 (MQ=255)                                 ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcgg                                                                                                         <  1:245442/122‑1 (MQ=255)                                 ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcgg                                                                                                         >  2:245442/1‑122 (MQ=255)                                 ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                        <  1:706186/139‑1 (MQ=255)                                                    tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                          <  2:1177229/86‑1 (MQ=255)                                                    tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGAGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                          >  1:1177229/1‑86 (MQ=255)                                                      aaCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          <  1:268599/116‑1 (MQ=255)                                                      aaCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          >  2:268599/1‑116 (MQ=255)                                                      aaCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAg                                                                   >  2:527233/1‑139 (MQ=255)                                                                   tctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                            <  1:66709/69‑1 (MQ=255)                                                                   tctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                            >  2:66709/1‑69 (MQ=255)                                                                   tctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCTGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                      <  2:1197224/139‑1 (MQ=255)                                                                    ctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                     <  1:1444767/139‑1 (MQ=255)                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     <  2:124644/73‑1 (MQ=255)                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     >  1:124644/1‑73 (MQ=255)                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGt                                                   >  1:851048/1‑139 (MQ=255)                                                                           tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                        >  1:893797/1‑97 (MQ=255)                                                                           tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                        <  2:893797/97‑1 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                          <  2:571572/61‑1 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                          >  1:571572/1‑61 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                            <  1:527233/139‑1 (MQ=255)                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                             <  2:1273107/88‑1 (MQ=255)                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                             >  1:1273107/1‑88 (MQ=255)                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCa                                          >  1:961290/1‑139 (MQ=255)                                                                                 ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCt                                                                      <  1:1168801/109‑1 (MQ=255)                                                                                 ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCt                                                                      >  2:1168801/1‑109 (MQ=255)                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTg                                                                                                                                >  1:704182/1‑47 (MQ=255)                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTg                                                                                                                                <  2:704182/47‑1 (MQ=255)                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                        >  1:638374/1‑87 (MQ=255)                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                        <  2:638374/87‑1 (MQ=255)                                                                                     gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTg                                    >  1:1597182/1‑139 (MQ=255)                                                                                       gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    >  1:1508932/1‑73 (MQ=255)                                                                                       gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  2:1508932/73‑1 (MQ=255)                                                                                         ggTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACa                                <  2:690657/139‑1 (MQ=255)                                                                                           tGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                 >  1:194154/1‑120 (MQ=255)                                                                                           tGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                 <  2:194154/120‑1 (MQ=255)                                                                                                    tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                     >  1:1402370/1‑139 (MQ=255)                                                                                                    tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                     >  1:1685369/1‑139 (MQ=255)                                                                                                    tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                     >  2:1007409/1‑139 (MQ=255)                                                                                                     gtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTa                    <  1:1007409/139‑1 (MQ=255)                                                                                                      tACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCt                                                                      <  1:1387787/88‑1 (MQ=255)                                                                                                      tACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCt                                                                      >  2:1387787/1‑88 (MQ=255)                                                                                                           tAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                     >  2:1183/1‑100 (MQ=255)                                                                                                           tAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                     <  1:1183/100‑1 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGAc                                                                                       <  1:1070414/63‑1 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGAc                                                                                       >  2:1070414/1‑63 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCa                                                           <  2:63688/91‑1 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCa                                                           >  1:63688/1‑91 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                            >  1:490252/1‑122 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                            <  2:490252/122‑1 (MQ=255)                                                                                                                  aTTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          >  1:392488/1‑56 (MQ=255)                                                                                                                  aTTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          <  2:392488/56‑1 (MQ=255)                                                                                                                  aTTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                                  <  1:1054463/112‑1 (MQ=255)                                                                                                                  aTTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                                  >  2:1054463/1‑112 (MQ=255)                                                                                                                   ttGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          >  2:41570/1‑55 (MQ=255)                                                                                                                   ttGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          <  1:41570/55‑1 (MQ=255)                                                                                                                   ttGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                            <  2:1579803/101‑1 (MQ=255)                                                                                                                   ttGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                            >  1:1579803/1‑101 (MQ=255)                                                                                                                     gTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCa    <  2:1597182/139‑1 (MQ=255)                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    >  2:602853/1‑41 (MQ=255)                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  1:602853/41‑1 (MQ=255)                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGAc                                 <  2:20192/108‑1 (MQ=255)                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGAc                                 >  1:20192/1‑108 (MQ=255)                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa  <  2:1402370/139‑1 (MQ=255)                                                                                                                       ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa  <  2:851048/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                  TTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGA  >  NC_000913/155808‑156064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑