Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,614,789	C→T	intergenic (‑86/‑15)	sad ← / → yneJ	succinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,614,789	0	C	T	100.0%	135.9 / NA	42	intergenic (‑86/‑15)	sad/yneJ	succinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/18);  total (24/18)

CGTGGCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTG  >  NC_000913/1614656‑1614920                                                                                                                                      |                                                                                                                                    cGTGGCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATc                                                                                                                                >  2:524300/1‑139 (MQ=255)     gCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgaga                                                                                                                            <  1:524300/139‑1 (MQ=255)                              ttGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGga                                                                                                   <  1:346599/139‑1 (MQ=255)                                       tAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCagc                                                                                                       <  1:1330258/126‑3 (MQ=255)                                       tAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                       >  2:1330258/1‑126 (MQ=255)                                           ggTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                       <  1:140060/122‑1 (MQ=255)                                           ggTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                       >  2:140060/1‑122 (MQ=255)                                               aTCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTg                                                                                  >  2:1414809/1‑139 (MQ=255)                                                    ggTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGaa                                                                             <  1:1414809/139‑1 (MQ=255)                                                         ctcCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                        <  2:1135248/139‑1 (MQ=255)                                                                    gTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAAcgcg                                                                                      <  2:447107/114‑1 (MQ=255)                                                                    gTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAAcgcg                                                                                      >  1:447107/1‑114 (MQ=255)                                                                      cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAg                                                           >  2:811609/1‑139 (MQ=255)                                                                                   tACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                        <  2:867129/113‑1 (MQ=255)                                                                                   tACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                        >  1:867129/1‑113 (MQ=255)                                                                                     cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                       <  2:1154626/80‑1 (MQ=255)                                                                                     cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                       >  1:1154626/1‑80 (MQ=255)                                                                                     cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                            >  1:255631/1‑139 (MQ=255)                                                                                     cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                            >  2:345856/1‑139 (MQ=255)                                                                                              aCTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTg                                   <  1:811609/139‑1 (MQ=255)                                                                                                 ctTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCg                                <  2:255631/139‑1 (MQ=255)                                                                                                    gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCg                             >  2:1641976/1‑139 (MQ=255)                                                                                                    gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCg                             >  1:851149/1‑139 (MQ=255)                                                                                                     cTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGa                            <  1:345856/139‑1 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                        >  2:1137094/1‑70 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                        <  1:1137094/70‑1 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGc                                                         <  2:1004764/85‑1 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGc                                                         >  1:1004764/1‑85 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGc                                                         >  1:1466700/1‑85 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGc                                                         <  2:1466700/85‑1 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCg                                >  1:1101454/1‑110 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCg                                <  2:1101454/110‑1 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGa                            >  2:1557826/1‑114 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGa                            <  1:1557826/114‑1 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt   >  1:536655/1‑139 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt   >  2:1375665/1‑139 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt   >  1:160555/1‑139 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt   >  1:1539418/1‑139 (MQ=255)                                                                                                                              cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt   >  1:616631/1‑139 (MQ=255)                                                                                                                               ttATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTg  >  1:1143342/1‑139 (MQ=255)                                                                                                                                tATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGaa                           <  2:794344/113‑1 (MQ=255)                                                                                                                                tATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGaa                           >  1:794344/1‑113 (MQ=255)                                                                                                                                      |                                                                                                                                    CGTGGCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTG  >  NC_000913/1614656‑1614920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑