Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,218	G→A	*397* (TAG→TAA)	ydeA →	arabinose efflux transporter, arabinose‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,218	0	G	A	100.0%	142.9 / NA	45	*397* (TAG→TAA)	ydeA	arabinose efflux transporter, arabinose‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/22);  total (23/22)

ATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACC  >  NC_000913/1618088‑1618347                                                                                                                                   |                                                                                                                                  aTCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                               >  1:1021963/1‑135 (MQ=255) aTCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                               <  2:1021963/135‑1 (MQ=255)  tCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGc                                                                                                                          >  1:763102/1‑139 (MQ=255)       tGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCAtt                                                                                                                     <  1:1336183/139‑1 (MQ=255)                          gTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                                                  <  2:763102/139‑1 (MQ=255)                                                     gCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGtt                                                                       >  1:695244/1‑139 (MQ=255)                                                                ccGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACt                                                            >  1:1580016/1‑139 (MQ=255)                                                                 cgcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                           <  2:1580016/139‑1 (MQ=255)                                                                    gTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                         <  2:233160/138‑1 (MQ=255)                                                                    gTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                         >  1:233160/1‑138 (MQ=255)                                                                                  tCATTATATTTCGCCGCTGGCCAGTGACACTCGGAGAACAGACGCAATCATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                                                  <  2:1095249/83‑1 (MQ=255)                                                                                  tCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                                                  >  1:1095249/1‑83 (MQ=255)                                                                                  tCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                                <  2:1505758/117‑1 (MQ=255)                                                                                  tCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                                >  1:1505758/1‑117 (MQ=255)                                                                                             cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTaa                                                                                                       >  2:153072/1‑67 (MQ=255)                                                                                             cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTaa                                                                                                       <  1:987196/67‑1 (MQ=255)                                                                                             cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTaa                                                                                                       >  2:987196/1‑67 (MQ=255)                                                                                             cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTaa                                                                                                       <  1:153072/67‑1 (MQ=255)                                                                                             cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATccc                               >  2:259992/1‑139 (MQ=255)                                                                                                cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                               <  1:689087/40‑1 (MQ=255)                                                                                                cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                                               >  2:689087/1‑40 (MQ=255)                                                                                                cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                                               <  2:736299/56‑1 (MQ=255)                                                                                                cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                                               >  1:736299/1‑56 (MQ=255)                                                                                                  cTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                                                  >  1:716699/1‑67 (MQ=255)                                                                                                  cTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                                                  <  2:716699/67‑1 (MQ=255)                                                                                                   tGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGa                         <  2:695244/139‑1 (MQ=255)                                                                                                    ggCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                                            <  1:359458/87‑1 (MQ=255)                                                                                                    ggCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                                            >  2:359458/1‑87 (MQ=255)                                                                                                          tGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCggg                                                                                                                 <  2:888369/44‑1 (MQ=255)                                                                                                          tGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCggg                                                                                                                 >  1:888369/1‑44 (MQ=255)                                                                                                          tGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGgaga                  >  1:697675/1‑139 (MQ=255)                                                                                                               cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                                               >  2:1271064/1‑41 (MQ=255)                                                                                                               cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                                               <  1:1271064/41‑1 (MQ=255)                                                                                                                    agaaCAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                                            <  2:1313034/71‑1 (MQ=255)                                                                                                                    agaaCAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                                            >  1:1313034/1‑71 (MQ=255)                                                                                                                       aCAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCa                                                                                 <  2:899270/63‑1 (MQ=255)                                                                                                                       aCAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCa                                                                                 >  1:899270/1‑63 (MQ=255)                                                                                                                         aGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCAc   <  1:259992/139‑1 (MQ=255)                                                                                                                          gACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                           >  1:607556/1‑82 (MQ=255)                                                                                                                          gACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                           <  2:607556/82‑1 (MQ=255)                                                                                                                          gACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCAcc  >  1:1129028/1‑139 (MQ=255)                                                                                                                           aCGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATAcc                                           <  2:604781/97‑1 (MQ=255)                                                                                                                           aCGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATAcc                                           >  1:604781/1‑97 (MQ=255)                                                                                                                                aTAATTGAAAGGCCCATTCGGGCCTTTTTTAGTGGTACGTTTTAATGATTTCCAGGAt                                                                             >  1:144374/1‑58 (MQ=255)                                                                                                                                aTAATTGAAAGGCCCATTCGGGCCTTTTTTAGTGGTACGTTTTAATGATTTCCAGGAt                                                                             <  2:144374/58‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  ATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACC  >  NC_000913/1618088‑1618347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑