Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,744	G→A	*187* (TAG→TAA)	speG →	spermidine N(1)‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,744	0	G	A	100.0%	161.1 / NA	50	*187* (TAG→TAA)	speG	spermidine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/24);  total (26/24)

ATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTT  >  NC_000913/1656622‑1656872                                                                                                                           |                                                                                                                                 aTGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcga                                                                                                                  <  1:773646/139‑1 (MQ=255)                            tatCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATtaa                                                                                                                            >  1:1156676/1‑102 (MQ=255)                            tatCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATtaa                                                                                                                            <  2:1156676/102‑1 (MQ=255)                                 tAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                >  1:520697/1‑93 (MQ=255)                                         ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      <  2:349020/111‑1 (MQ=255)                                         ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      >  1:349020/1‑111 (MQ=255)                                         ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGttt                                                                          >  2:920681/1‑139 (MQ=255)                                         ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGttt                                                                          >  2:1514561/1‑139 (MQ=255)                                         ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGttt                                                                          >  1:1368805/1‑139 (MQ=255)                                             cATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                      <  1:920681/139‑1 (MQ=255)                                                        tCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATcaac                                                           >  1:429632/1‑139 (MQ=255)                                                             cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                <  2:875210/65‑1 (MQ=255)                                                             cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                >  1:875210/1‑65 (MQ=255)                                                             cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACAtt                                                                                                                              <  1:1362114/67‑1 (MQ=255)                                                             cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACAtt                                                                                                                              >  2:1362114/1‑67 (MQ=255)                                                             cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      >  2:1170725/1‑91 (MQ=255)                                                             cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      <  1:1170725/91‑1 (MQ=255)                                                                        ggCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAg                                           <  1:1514561/139‑1 (MQ=255)                                                                           agagCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGgtg                                                                                                 <  1:190391/82‑1 (MQ=255)                                                                           agagCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGgtg                                                                                                 >  2:190391/1‑82 (MQ=255)                                                                             agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATtaataat                                                                                                                        >  1:439831/1‑57 (MQ=255)                                                                             agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATtaataat                                                                                                                        <  2:439831/57‑1 (MQ=255)                                                                             agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTg                                                                                                       <  2:774118/74‑1 (MQ=255)                                                                             agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTg                                                                                                       >  1:774118/1‑74 (MQ=255)                                                                             agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCAc                                      >  1:1453315/1‑139 (MQ=255)                                                                             agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCAc                                      >  1:155322/1‑139 (MQ=255)                                                                             agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCAc                                      <  1:1523985/139‑1 (MQ=255)                                                                               cacaAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTcc                                                                    <  1:1176230/107‑1 (MQ=255)                                                                               cacaAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTcc                                                                    >  2:1176230/1‑107 (MQ=255)                                                                                 caAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                >  2:1016817/1‑45 (MQ=255)                                                                                    aaCACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATtaa                                                                                                                            >  1:139942/1‑46 (MQ=255)                                                                                    aaCACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATtaa                                                                                                                            <  2:139942/46‑1 (MQ=255)                                                                                    aaCACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                      <  1:1155902/100‑1 (MQ=255)                                                                                    aaCACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACt                                                                      >  2:1155902/1‑100 (MQ=255)                                                                                         cGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      <  1:65643/63‑1 (MQ=255)                                                                                         cGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      >  2:65643/1‑63 (MQ=255)                                                                                         cGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGa                                              <  2:713807/119‑1 (MQ=255)                                                                                         cGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGa                                              >  1:713807/1‑119 (MQ=255)                                                                                           ggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGAc                        >  2:367632/1‑139 (MQ=255)                                                                                            gTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACt                       <  1:137380/139‑1 (MQ=255)                                                                                            gTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACt                       <  2:1453315/139‑1 (MQ=255)                                                                                                           aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGct        <  2:155322/139‑1 (MQ=255)                                                                                                           aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGct        <  2:94837/139‑1 (MQ=255)                                                                                                            gCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGctc       >  2:1453368/1‑139 (MQ=255)                                                                                                             ccGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      <  1:804124/43‑1 (MQ=255)                                                                                                             ccGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      >  2:804124/1‑43 (MQ=255)                                                                                                               gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      >  1:1272344/1‑41 (MQ=255)                                                                                                               gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                      <  2:1272344/41‑1 (MQ=255)                                                                                                               gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGt    >  2:358971/1‑139 (MQ=255)                                                                                                                 ccGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGttt  <  1:367632/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                 ATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTT  >  NC_000913/1656622‑1656872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑