Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,581	G→A	G295R (GGA→AGA)	ynfM →	putative arabinose efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,581	0	G	A	100.0%	145.1 / NA	45	G295R (GGA→AGA)	ynfM	putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

TCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTC  >  NC_000913/1670455‑1670706                                                                                                                               |                                                                                                                              tcgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGacca                                                                                                                   <  2:1012222/139‑1 (MQ=255)         cACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTAt                                                                                                           >  1:411245/1‑139 (MQ=255)              tGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      <  2:411245/139‑1 (MQ=255)                      tACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAg                                                                                              <  1:130520/139‑1 (MQ=255)                          tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGaccacc                                                                                                                 >  2:92545/1‑116 (MQ=255)                          tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGaccacc                                                                                                                 <  1:92545/116‑1 (MQ=255)                          tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAt                                                                                          >  2:975707/1‑139 (MQ=255)                          tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAt                                                                                          >  1:1333934/1‑139 (MQ=255)                                      tGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                              <  2:1333934/139‑1 (MQ=255)                                        aTGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      >  2:781856/1‑113 (MQ=255)                                        aTGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      <  1:781856/113‑1 (MQ=255)                                           ctctCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACggggg                                                                         >  1:1505703/1‑139 (MQ=255)                                              tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                      >  2:1327153/1‑139 (MQ=255)                                                          gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACGATGACCACCCGCTATGGGCGTGGTc                                                                                                 <  2:1498376/100‑1 (MQ=255)                                                          gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATggg                                                                                                        <  2:661352/93‑1 (MQ=255)                                                          gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATggg                                                                                                        >  1:661352/1‑93 (MQ=255)                                                          gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                      <  1:288233/127‑1 (MQ=255)                                                          gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                      >  2:288233/1‑127 (MQ=255)                                                          gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGtt                                                          >  1:1377646/1‑139 (MQ=255)                                                          gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGAACGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                 >  1:1498376/1‑100 (MQ=255)                                                                cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                    <  1:975707/139‑1 (MQ=255)                                                                                  ttatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                  >  2:1339329/1‑139 (MQ=255)                                                                                        cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGc                            >  2:1219820/1‑139 (MQ=255)                                                                                             ttATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      >  1:1412535/1‑60 (MQ=255)                                                                                             ttATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      <  2:1412535/60‑1 (MQ=255)                                                                                               aTTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGaccac                                                                                                                  >  1:1003952/1‑46 (MQ=255)                                                                                               aTTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGaccac                                                                                                                  <  2:1003952/46‑1 (MQ=255)                                                                                               aTTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCtt                     >  1:2850/1‑139 (MQ=255)                                                                                               aTTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTG‑TTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCttt                    >  2:1437527/1‑139 (MQ=255)                                                                                                     ccGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCgg               <  2:1505703/139‑1 (MQ=255)                                                                                                      cGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                      <  2:992795/83‑1 (MQ=255)                                                                                                      cGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                      >  1:992795/1‑83 (MQ=255)                                                                                                         tACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                    <  2:1684912/98‑1 (MQ=255)                                                                                                         tACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGCGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                    >  1:1684912/1‑98 (MQ=255)                                                                                                                aGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTG‑TTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtctt   <  1:1437527/139‑1 (MQ=255)                                                                                                                 gCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtctt   <  1:1339329/139‑1 (MQ=255)                                                                                                                  cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      <  1:698430/39‑1 (MQ=255)                                                                                                                  cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      >  2:698430/1‑39 (MQ=255)                                                                                                                  cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttc  >  1:693920/1‑139 (MQ=255)                                                                                                                   tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      >  2:1366993/1‑38 (MQ=255)                                                                                                                   tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                      <  1:1366993/38‑1 (MQ=255)                                                                                                                   tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                <  2:376509/124‑1 (MQ=255)                                                                                                                   tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                >  1:376509/1‑124 (MQ=255)                                                                                                                          aaGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtgat                                                   <  2:209094/82‑1 (MQ=255)                                                                                                                          aaGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtgat                                                   >  1:209094/1‑82 (MQ=255)                                                                                                                               |                                                                                                                              TCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTC  >  NC_000913/1670455‑1670706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑