| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,699,528 | G→A | V58V (GTG→GTA) | malX → | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,699,528 | 0 | G | A | 100.0% | 199.1 / NA | 61 | V58V (GTG→GTA) | malX | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/29); total (32/29) | |||||||||||
TTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCAT > NC_000913/1699397‑1699662 | ttcCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg < 1:303430/139‑1 (MQ=255)ttcCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg < 1:1571796/139‑1 (MQ=255) tcCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGc > 1:1056086/1‑139 (MQ=255) agcagTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTAt < 2:1056086/139‑1 (MQ=255) cAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTgg < 1:1369220/139‑1 (MQ=255) aaCCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg < 2:1620592/122‑1 (MQ=255) aaCCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg > 1:1620592/1‑122 (MQ=255) cATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAg > 1:1192019/1‑139 (MQ=255) gTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGt < 1:1555152/139‑1 (MQ=255) ggCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGc < 2:1406298/105‑1 (MQ=255) ggCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGc > 1:1406298/1‑105 (MQ=255) gTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg > 2:446163/1‑92 (MQ=255) gTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg < 1:446163/92‑1 (MQ=255) cGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg > 2:1263929/1‑86 (MQ=255) cGGCATTATGCTCGGCATTGGTAGTTCTCGTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg < 1:1263929/86‑1 (MQ=255) ttATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGc < 1:701002/118‑1 (MQ=255) ttATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGc > 2:701002/1‑118 (MQ=255) tGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTgg < 2:438664/92‑1 (MQ=255) tGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTgg > 1:438664/1‑92 (MQ=255) ggCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg > 1:707175/1‑73 (MQ=255) ggCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAgg < 2:707175/73‑1 (MQ=255) ggCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGc < 1:401724/74‑1 (MQ=255) ggCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGc > 2:401724/1‑74 (MQ=255) cATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTc > 2:714383/1‑127 (MQ=255) cATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTc < 1:714383/127‑1 (MQ=255) tGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCt < 1:764323/98‑1 (MQ=255) tGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCt > 2:764323/1‑98 (MQ=255) tctcTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGc > 2:1033499/1‑139 (MQ=255) tctTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTg > 2:1170154/1‑139 (MQ=255) tAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGc > 2:126350/1‑139 (MQ=255) gcagcCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTg > 2:777723/1‑50 (MQ=255) gcagcCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTg < 1:777723/50‑1 (MQ=255) agcCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTgg < 1:126350/139‑1 (MQ=255) atgTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGa > 2:181363/1‑139 (MQ=255) aTAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGttt < 2:1369747/86‑1 (MQ=255) aTAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGttt > 1:1369747/1‑86 (MQ=255) tAACCCTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCGGCTGGGCCTGGCACGCTAAAATaa < 1:1033499/139‑1 (MQ=255) ccTGATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGc > 2:1407084/1‑139 (MQ=255) gATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGt > 2:1290428/1‑52 (MQ=255) gATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGt < 1:1290428/52‑1 (MQ=255) gATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTcctgcctg > 2:174479/1‑86 (MQ=255) gATCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTcctgcctg < 1:174479/86‑1 (MQ=255) aTCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAg > 2:462307/1‑139 (MQ=255) aTCCCGGTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAg > 2:968494/1‑139 (MQ=255) gTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTcctgc < 1:884706/76‑1 (MQ=255) gTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTcctgc > 2:884706/1‑76 (MQ=255) gTCCTGGGCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCAt > 1:40016/1‑139 (MQ=255) ggtaaCCCCGTATTGCAGGCTATCTTTACCTGGATGAg > 1:1174301/4‑38 (MQ=255) ggCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAg < 2:1174301/38‑1 (MQ=255) ggCAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTg > 1:1668050/1‑139 (MQ=255) cAACCCCGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGc < 1:1170154/139‑1 (MQ=255) ccccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTc > 2:985562/1‑70 (MQ=255) ccccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTc < 1:985562/70‑1 (MQ=255) ccccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATTCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTc > 1:1638422/1‑139 (MQ=255) ccccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGc < 2:247614/92‑1 (MQ=255) ccccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGc > 1:247614/1‑92 (MQ=255) ccccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTc > 2:1460612/1‑139 (MQ=255) ccccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTc > 1:1687101/1‑139 (MQ=255) ccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATTCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCAt < 2:1638422/139‑1 (MQ=255) ccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCAt < 2:1687101/139‑1 (MQ=255) ccGTATTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCAt < 1:968494/139‑1 (MQ=255) | TTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGCAACCCCGTGTTGCAGGCTATCTTTACCTGGATGAGTAAGATTGGCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCAT > NC_000913/1699397‑1699662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |