| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,756,750 | A→T | K351N (AAA→AAT) | pykF → | pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,756,750 | 0 | A | T | 97.8% | 145.9 / ‑3.5 | 46 | K351N (AAA→AAT) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (21/24); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01 | |||||||||||
CTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATC > NC_000913/1756614‑1756885 | cTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCt > 2:614061/1‑139 (MQ=255) gCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCa > 1:828709/1‑139 (MQ=255) gTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTa > 2:1439659/1‑139 (MQ=255) gCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGaa < 1:614061/139‑1 (MQ=255) cGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCgtggt < 2:828709/139‑1 (MQ=255) cGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCgtggt < 2:701640/139‑1 (MQ=255) cGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTgcg > 1:914116/1‑97 (MQ=255) cGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTgcg < 2:914116/97‑1 (MQ=255) cGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATg > 2:1150351/1‑115 (MQ=255) cGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATg < 1:1150351/115‑1 (MQ=255) gCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaa > 2:1021929/1‑139 (MQ=255) aaGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt < 2:749271/139‑1 (MQ=255) gTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATg < 1:1021929/139‑1 (MQ=255) cTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTcc > 2:1219398/1‑139 (MQ=255) cATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTg > 2:428866/1‑139 (MQ=255) ggCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAc < 1:803716/111‑1 (MQ=255) ggCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAc > 2:803716/1‑111 (MQ=255) cGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGt < 1:1219398/139‑1 (MQ=255) aCCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAg > 2:800202/1‑82 (MQ=255) aCCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAg < 1:800202/82‑1 (MQ=255) aTCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTg < 1:1237270/139‑1 (MQ=255) tACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGc < 1:428866/139‑1 (MQ=255) tACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGc > 1:1290345/1‑139 (MQ=255) accgaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAGCt < 1:88594/104‑1 (MQ=255) accgaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt > 2:88594/1‑104 (MQ=255) accgaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAg > 1:1133902/1‑135 (MQ=255) accgaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAg < 2:1133902/135‑1 (MQ=255) accgaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCg > 2:1433614/1‑139 (MQ=255) gaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGc < 1:781744/107‑1 (MQ=255) gaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGc > 2:781744/1‑107 (MQ=255) gTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCg < 1:836211/68‑1 (MQ=255) gTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCg > 2:836211/1‑68 (MQ=255) tgatgaACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGa > 2:934125/1‑108 (MQ=255) tgatgaACAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGa < 1:934125/108‑1 (MQ=255) aaCAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTcgcg < 1:1439659/139‑1 (MQ=255) cAGCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCa < 1:1504315/139‑1 (MQ=255) gCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg > 2:324225/1‑105 (MQ=255) gCCGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg < 1:324225/105‑1 (MQ=255) cGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGt > 2:905308/1‑101 (MQ=255) cGTCTCGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGt < 1:905308/101‑1 (MQ=255) tctcGAGTTCAACAATGACAACCGTAATCTGCGCATTACCg > 2:75119/1‑41 (MQ=255) tctcGAGTTCAACAATGACAACCGTAATCTGCGCATTACCg < 1:75119/41‑1 (MQ=255) tctcGAGTTCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGt > 1:730836/1‑139 (MQ=255) ttCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGa < 2:1348659/59‑1 (MQ=255) ttCAACAATGACAACCGTAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGa > 1:1348659/1‑59 (MQ=255) tAATCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATc < 2:1290345/139‑1 (MQ=255) | CTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATC > NC_000913/1756614‑1756885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |